Garcia, Maíra TerraWard, Rafael Araújo da CostaGonçalves, Nathália Maria FerreiraPedroso, Lara Luise CastroSilva Neto, José Vieira daFerreira-Strixino, JulianaJunqueira, Juliana Campos2025-04-222025-04-22https://repositorio.univap.br/handle/123456789/862Photodynamic therapy (PDT) mediated by Fotoenticine® (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium Streptococcus mutans. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of mutans streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.pdfenArtigos de PeriódicosMaíra Terra GarciaRafael Araújo da Costa WardNathália Maria Ferreira GonçalvesLara Luise Castro PedrosoJosé Vieira da Silva NetoJuliana Ferreira StrixinoJuliana Campos Junqueirahttps://doi.org/10.3390/pharmaceutics13111907Photodynamic therapyDental cariesOral biofilmsFotoenticineChlorineMethylene blueGARCIA, M. T. et al. Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine. Pharmaceutics, [s. l.], v. 13, n. 11, p. 1907, 2021. Disponível em: https://www.mdpi.com/1999-4923/13/11/1907.Department of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESPDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESPDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESPDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESPAssociate Laboratory of Sensors and Materials/LABAS, National Institute for Space ResearchPhotobiology Applied to Health, Research and Development Institute IP&D, University of Vale do Paraiba/UNIVAPDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP