Machado, Lucas SimõesFerreira, Paula ScanavezPires, Marina RodriguesBim, Larissa ValdemarinOliveira, Natália Heloísa deSalles, Geisa RodriguesFerreira, Natalia Dall'AgnolCruz, Elisa MarozziPorcionatto, Marimelia Aparecida2026-02-132026-02-13https://repositorio.univap.br/handle/123456789/1153Animal models, especially rodents, used to study neurodevelopment have significantly advanced our comprehension of cellular and molecular mechanisms. Nevertheless, differences in species-specific structures, gestation periods, and interneuronal connections limit animal models’ ability to represent human neurodevelopment accurately. The unique characteristics of primate neural progenitor cells (NPCs) enable cortex expansion with gyrus formation, which does not occur in lissencephalic animals, like rodents. Therefore, there is a need for novel in vitro models using human cells that recapitulate the complexity of human brain development. Along with organoids, 3D bioprinting offers a platform for creating more complex in vitro models. We developed, extensively characterized, and successfully used a GeltrexTM/GelMA hydrogel blend to bioprint human induced pluripotent stem cells-derived NPCs (hNPCs). We show that 3D bioprinted hNPCs can selforganize, revealing key features of a neurogenic niche, including proliferation, differentiation, and migration, remaining viable for over 110 days. Within the first 20 days, bioprinted constructs showed the formation of positive cell clusters for the neurogenic niche cell markers FABP7, NESTIN, and GFAP. Clusters were interconnected by process bundles supporting cell migration. The cells proliferated within the clusters, and over time, NPCs originated TUBB3þ neurons with long axonal tracts, prominent around the clusters. We propose this as a 4D model to study neurogenic niches’ key cellular and molecular features in a 3D bioprinted scaffold, adding time as the fourth dimension. Neuronal maturation in this dynamic model recapitulates key neurogenic niche properties, making it suitable for neurodevelopmental disease modeling and drug screening.pdfen-USArtigos de PeriódicosLucas Simões MachadoPaula Scanavez FerreiraMarina Rodrigues PiresLarissa Valdemarin BimNatalia Heloísa de OliveiraGeisa Rodrigues SallesNatalia Dall’Agnol FerreiraElisa Marozzi CruzMarimelia Aparecida Porcionattohttps://doi.org/10.1063/5.0276704OrganoidsMigrationHydrogelsMACHADO, L.; FERREIRA, P.; PIRES, M.; BIM, L.; DE OLIVEIRA, N.; SALLES, G.; FERREIRA, N.; CRUZ, E.; PORCIONATTO, M. 3D bioprinted human iPSC-derived neural progenitor cells as a novel platform for studying neurogenic niche. APL BIOENGINEERING, [S. l.], v. 9, n. 3, 1 set. 2025. https://doi.org/10.1063/5.0276704.Universidade de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade do Vale do ParaíbaUniversidade Federal de São PauloUniversidade Federal de São PauloUniversidade Federal de São Paulo