Bronze, Carla da Silveira e OliveiraGrisante, Letícia Adrielly DiasAraújo, Juliani Caroline Ribeiro deGuardia, Rafaella SouzaVicuna, Iranel de las Nievez GonzálezOliveira, Ivone Regina deVasconcellos, Luana Marotta Reis de2026-01-132026-01-13https://repositorio.univap.br/handle/123456789/1084Objective: The objective of this study was to evaluate the bone regeneration potential of CAC-based scaffolds, with or without mesenchymal stem cells (MSC), in bone defects created in rat femurs. Material and Methods: Forty-eight CAC scaffolds and their blends of tricalcium phosphate (TCP), zinc oxide (ZNO), and zirconia (ZIRC) were produced, with half of these incorporated with MSC. Twenty-three Wistar rats were used, with 3 for MSC isolation and 20 for creating bone defects in both femurs. Five animals were assigned to each group, and during the defect surgery and material insertion, the animals received MSC-incorporated scaffolds on the left side and non-incorporated scaffolds on the right side, with the same type of material used in each animal to avoid different systemic effects (n=5); they were euthanized 21 days after the surgical procedure. Results: In the scanning electron microscopy analysis of the scaffolds, structures with open and interconnected pores, as well as cell adhesion, were observed in all groups. In the histological analysis, all groups showed newly formed bone trabeculae interspersed with bone marrow cells and connective tissue. Conclusion: In the histomorphometry, for the scaffolds not incorporated with MSC, the ZIRC group showed greater bone formation, and in the MSC- incorporated scaffolds, the TCP group demonstrated better results, both exhibiting a statistically significant difference from the other groups (p<0.05).PDFen-USArtigos de PeriódicosBrazilian Dental Science10.4322/bds.2025.e4296Biocompatible materialsBone cementsBone regenerationBone tissueMesenchymal stem cellsBRONZE, C. S. et al. A new approach in bone tissue regeneration: in vivo study of the impact of calcium aluminate cement scaffolds incorporated with mesenchymal cells. Brazilian Dental Science, v. 8, n. 1, p. 1-14, 2025. Disponível em: https://ojs.ict.unesp.br/index.php/cob/article/view/4296.Universidade do Vale do ParaíbaUniversidade Estadual Paulista