Oliveira Junior, Benedito Marcio deTeodoro, Jéssica Beatriz MirandaAmbrósio, Jéssica Aparecida RibeiroGonçalves, Érika PetersonBeltrame Junior, MiltonMarcolino, Luciana Maria CortezPinto, Juliana GuerraFerreira-Strixino, JulianaSimioni, Andreza Ribeiro2023-09-262023-09-26https://repositorio.univap.br/handle/123456789/319Development delivery systems, such as nanoparticles, represent a growing area in biomedical research. Nanoparticles (NP) were prepared using a double-emulsion method to load zinc(II) phthalocyanine (ZnPc). NP were obtained using poly (lactic acid) (PLA). ZnPc is a second generation of photosensitizer used in photodynamic therapy (PDT). ZnPc loaded PLA nanoparticles (NPLAZnPc) were prepared by double-emulsion method, characterized and available in cellular culture. The mean nanoparticle size presented particle size was 384.7 ± 84.2 nm with polydispersity index (PDI) of 0.150 ± 0.015, and the encapsulation efficiency was of 83%. The nanoparticle formulations presented negative zeta potential values (27.5 ± 1.0 mV), explaining their colloidal stability. ZnPc loaded nanoparticles maintain its photophysical behavior after encapsulation. Photosensitizer release from nanoparticles was sustained over 168 h with a biphasic ZnPc release profile. An in vitro phototoxic effect in range of 80% was observed in 9 L/ LacZ gliosarcoma cells at laser light doses (10 J cm2) with 3.0 mg mL1 of NPLA-ZnPc. All the physical–chemical, photophysical and photobiological measurements performed allow us to conclude that ZnPc loaded PLGA nanoparticles is a promising drug deliverysystem for PDT.pdfenAcesso AbertoArtigos de PeriódicosOliveira Junior, Benedito Marcio deTeodoro, Jéssica Beatriz MirandaAmbrósio, Jéssica Aparecida RibeiroGoncalves, Erika PetersonBeltrame Junior, MiltonMarcolino, Luciana Maria CortezPinto, Juliana GuerraFerreira-Strixino, JulianaSimioni, Andreza Ribeirohttps://doi.org/10.1080/09205063.2021.1980359Photodynamic therapyPolymeric nanoparticlesZinc phthalocyanineOLIVEIRA JUNIOR, B. M., TEODORO, J. B. M., AMBRÓSIO, J. A. R., GONÇALVES, E. P., BELTRAME JUNIOR, M., MARCOLINO, L. M. C.; PINTO, J. G.; FERREIRA-STRIXINO, J.; SIMIONI, A. R. Zinc pthalocyanine loaded poly (lactic acid) nanoparticles by double emulsion methodology for photodynamic therapy against 9 L/LacZ gliosarcoma cells. Journal of Biomaterials Science-Polymer Edition, v. 33, n. 1, p. 93-109, Sep. 2021. (18/18531-6)Univap