Pereira, André Henrique CorreiaMarcolino, Luciana Maria CortezPinto, Juliana GuerraStrixino, Juliana Ferreira2025-05-272025-05-27https://repositorio.univap.br/handle/123456789/974Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 μg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 μg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed.pdfenArtigos de PeriódicosMDPIAndré Henrique Correia PereiraLuciana Maria Cortez MarcolinoJuliana Guerra PintoJuliana Ferreira-Strixinohttps://doi.org/10.3390/antibiotics10060634Photodynamic therapyCurcuminAmastigotesMacrophagesCutaneous leishmaniasisPEREIRA, André Henrique Correia; MARCOLINO, Luciana Maria Cortez; PINTO, Juliana Guerra; FERREIRA-STRIXINO, Juliana. Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes. Antibiotics, [s. l.], v. 10, n. 6, p. 634, 25 maio 2021. DOI 10.3390/antibiotics10060634. Disponível em: https://www.mdpi.com/2079-6382/10/6/634.University of Vale do ParaíbaUniversity of Vale do ParaíbaUniversity of Vale do ParaíbaUniversity of Vale do Paraíba