Gelain, LucasKingma, EstherPradella, José Geraldo da CruzCosta, Aline Carvalho daWielen, Luuk Van DerGulik, Walter Martin van2025-04-092025-04-09https://repositorio.univap.br/handle/123456789/831Carbon-limited chemostat cultures were performed using different carbon sources (glucose, 10 and 20 g/L; sucrose, 10 g/L; fructose/glucose, 5.26/5.26 g/L; carboxymethyl cellulose, 10 g/L; and carboxymethyl cellulose/glucose, 5/5 g/L) to verify the capability of the wild type strain Trichoderma harzianum to produce extracellular enzymes. All chemostat cultures were carried out at a fixed dilution rate of 0.05 h . Experiments using glucose, fructose/glucose and sucrose were performed in duplicate. Glucose condition was found to induce the production of enzymes that can catalyse the hydrolysis of p-nitro- phenyl-b-D-glucopyranoside (PNPGase). A concentration of 20 g/L of glucose in the feed provided the highest productivity (1048 ± 16 U/mol h). Extracellular polysaccharides were considered the source of inducers. Based on the obtained results, a new PNPGase production process was developed using mainly glucose. This process raises interesting possibilities of synthesizing the inducer substrate and the induced enzymes in a single step using an easily assimilated carbon source under carbon-limited conditions.PDFen-USArtigos de PeriódicosFungal Biology10.1016/j.funbio.2020.10.008Enzyme productionContinuous fermentation processCarbon limitationPNPGaseGlucoseExtracellular polysaccharidesGELAIN, Lucas et al. Continuous production of enzymes under carbon-limited conditions by Trichoderma harzianum P49P11. Fungal Biology, v. 125, v. 3, p. 177-183, 2021. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1878614620301604.Universidade do Vale do ParaíbaDelft University of TechnologyUniversity of LimerickUniversidade Federal de São Paulo