Castilho, Maiara LimaJesus, Viviane Paula dos SantosVieira, Paula Fonseca AntunesHewitt, Kevin CecilRaniero, Leandro José2025-06-122025-06-12https://repositorio.univap.br/handle/123456789/995To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 μg/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency.PDFen-USArtigos de PeriódicosPhotodiagnosis and Photodynamic Therapy10.1016/j.pdpdt.2021.102186Bifunctional nanoprobesEpidermal growth factorChlorin e6Triple-negative breast cancerTheranosticPhotodynamic TherapyCASTILHO, M. L. et al. Chlorin e6-EGF conjugated gold nanoparticles as a nanomedicine based therapeutic agent for triple negative breast cancer. Photodiagnosis and Photodynamic Therapy, v. 33, p. 1-7, 2021. Disponível em:Universidade do Vale do ParaíbaDalhousie University