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Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications

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dc.contributor.author Toledo, Maria Cristina Modesto Clementino de
dc.contributor.author Abreu, Alexandro da Silva
dc.contributor.author Carvalho, Janicy Arantes
dc.contributor.author Ambrósio, Jéssica Aparecida Ribeiro
dc.contributor.author Godoy, Daniele da Silva
dc.contributor.author Pinto, Bruna Cristina dos Santos
dc.contributor.author Beltrame Junior, Milton
dc.contributor.author Simioni, Andreza Ribeiro
dc.date.accessioned 2023-09-26T14:50:34Z
dc.date.available 2023-09-26T14:50:34Z
dc.identifier.uri http://200.144.97.21:8080/xmlui/handle/123456789/321
dc.description.abstract Background: Photodynamic Therapy (PDT) is a modality for the treatment of neoplastic tissues, which is based on the administration of a phototherapeutic agent and light irradiation at an appropriate wavelength, aiming to locate and destroy the target cell with the formation of reactive oxygen species. Nanoencapsulation technology presents itself as a tool for incorporation of bioactive substances aiming to improve their solubility in physiological environment, obtain a longer circulation time in the organism, administration of lower dosages and the minimization of side effects. The present work aimed at the development of poly (lactic acid-glycolic acid) (PLGA) nanoparticles coated with polyelectrolyte film layers for encapsulating zinc phthalocyanine tetrasulfonated (ZnPcSO4) as a bioactive substance model. Methods: PLGA nanoparticles were produced by the double emulsion/solvent evaporation technique and polyelectrolytic coating was performed using polyalkylamine hydrochloride (PAH) as a weak polycation and poly (4- styrene sulfonate) (PSS) as a strong polyanion by layer-by-layer self-assembly technique (known as layer-by-layer-LbL). The nanoparticulate system was studied by scanning electron microscopy, steady-state, and their biological activity was evaluated using in vitro cancer cell lines by classical MTT assay. Results: The polyelectrolytic PLGA nanoparticles had an average diameter of 384.7 ± 138.6 nm, restricted distribution size with a polydispersity index. The obvious change in zeta potential indicates successful alternation in polycation (PAH) and polyanion (PSS) deposition directly in PLGA nanoparticles. Scanning electron microscopy (SEM) analysis showed that the formed system had morphology spherical, typical of these release systems. The loading efficiency was 82.1 % ± 1.2 %. The polyelectrolytic nanoparticles loaded with phthalocyanine maintained their photophysical behavior after encapsulation. Cell viability was determined, obtaining 90 % cell death. Conclusions: Therefore, the presented work depicts ZnPcSO4-loaded polyelectrolytic PLGA nanoparticles as a promise drug delivery system for phototherapeutic agent, which are thus expected to have superior therapeutic efficiency than free drug. pt_BR
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) pt_BR
dc.description.uri Projeto numero 2018/18531-6. pt_BR
dc.format.mimetype pdf pt_BR
dc.language.iso en pt_BR
dc.publisher Elsevier pt_BR
dc.rights Acesso Aberto pt_BR
dc.title Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications pt_BR
dc.type Artigos de Periódicos pt_BR
dc.rights.holder Toledo, Maria Cristina Modesto Clementino de pt_BR
dc.rights.holder Abreu, Alexandro da Silva pt_BR
dc.rights.holder Carvalho, Janicy Arantes pt_BR
dc.rights.holder Ambrósio, Jéssica Aparecida Ribeiro pt_BR
dc.rights.holder Godoy, Daniele da Silva pt_BR
dc.rights.holder Pinto, Bruna Cristina dos Santos pt_BR
dc.rights.holder Beltrame Junior, Milton pt_BR
dc.rights.holder Simioni, Andreza Ribeiro pt_BR
dc.identifier.doi https://doi.org/10.1016/j.pdpdt.2020.101966
dc.subject.keyword Nanoparticles pt_BR
dc.subject.keyword Phthalocyanine pt_BR
dc.subject.keyword Photodynamic therapy pt_BR
dc.subject.keyword Polyelectrolyte pt_BR
dc.description.physical 10 f. pt_BR
dc.identifier.bibliographicCitation TOLEDO, M. C. M. C.; ABREU, A. D. S.; CARVALHO, J. A.; AMBRÓSIO, J. A. R.; GODOY, D. S.; PINTO, B. C. S.; BELTRAME JUNIOR, M.; SIMIONI, A. R. Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications. Photodiagnosis and Photodynamic Therapy, v. 32, p. 1-10, 2020. (18/18531-6).https://www.sciencedirect.com/science/article/abs/pii/S1572100020303203 pt_BR
dc.date.issued2 2020-08-21
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR
dc.identifier.affiliation Universidade de São Paulo pt_BR
dc.identifier.affiliation Universidade de São Paulo pt_BR
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR
dc.identifier.affiliation Universidade do Vale do Paraíba pt_BR


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