REPOSITÓRIO INSTITUCIONAL DA UNIVERSIDADE DO VALE DO PARAÍBA
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Submissões Recentes
Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases
(Royal Society of Chemistry) Cairo, João Paulo L. Franco; Mandelli, Fernanda; Tramontina, Robson; Cannella, David; Paradisi, Alessandro; Ciano, Luisa; Ferreira, Marcel R.; Liberato, Marcelo V.; Brenelli, Lívia B.; Gonçalves, Thiago A.; Rodrigues, Gisele N.; Alvarez, Thabata M.; Mofatto, Luciana S.; Carazzolle, Marcelo F.; Pradella, Jose Geraldo da Cruz; Leme, Adriana F. Paes; Leonardo, Ana M. Costa; Neto, Mário Oliveira; Damasio, André; Davies, Gideon J.; Felby, Claus; Walton, Paul H.; Squina, Fabio M.
Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown enzymatic activities in their digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays a previously unknown role in plant biomass degradation. We show that CgSOD-1 transcripts and peptides are up-regulated in response to an increased level of lignocellulose recalcitrance and that CgSOD-1 localizes in the lumen of the fore- and midguts of C. gestroi together with termite main cellulase, CgEG-1-GH9. CgSOD-1 boosts the saccharification of polysaccharides by CgEG-1-GH9. We show that the boosting effect of CgSOD-1 involves an oxidative mechanism of action in which CgSOD-1 generates reactive oxygen species that subsequently cleave the polysaccharide. SOD-type enzymes constitute a new addition to the growing family of oxidases, ones which are up-regulated when exposed to recalcitrant polysaccharides, and that are used by Nature for biomass degradation.
Local envenomation caused by a bioactive peptide fraction of Bothrops jararaca snake venom induces leukocyte influx in the lung and changes in pulmonary mechanics
(Elsevier) Silva, Carlos Alberto; Querobino, Samyr Machado; Silva, Cesar Augusto Melo; Costa, Maricilia Silva; Oliveira, Luis Vicente Franco; Zamuner, Stella Regina
The crude venom of the Bothrops jararaca snake (Bj-CV) is a complex mixture of biologically active proteins that includes a variety of peptides in the low molecular weight fraction (Bj-PF). We investigated how an intramuscular injection of Bj-CV (1.2 mg kg−1) and Bj-PF (0.24 mg kg−1) influenced lung mechanics and lung and muscle inflammation in male Swiss mice 15 min, 1, 6, and 24 h after inoculation. Pressure dissipation against lung resistive components (ΔP1) rose significantly from 1 to 24 h after Bj-CV and 6–24 h after Bj-PF inoculation. Both Bj-CV and Bj-PF increased the total pressure variation of the lung (ΔPtot) 24 h after injection. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Furthermore, intramuscular inoculation of Bj-CV and Bj-PF resulted in an increase in muscle and pulmonary inflammation, as evidenced by an increase in leukocyte influx when compared to the control group. Finally, both Bj-CV and Bj-PF cause acute lung injury, as shown by pulmonary inflammation and decreased lung mechanics. Furthermore, the fact that Bj-PF produces mechanical alterations in the lungs and muscular inflammation implies that non-enzymatic compounds can cause inflammation.
Estimation of the DNA damage in Girardia tigrina (Girard, 1850) after exposure to radiation under extreme conditions
(Brazilian Journals Publicações de Periódicos) Benitz, Tabatha; Oliveira, Matheus Salgado de; Lopes, Karla Andressa Ruiz; Soares, Cristina Pacheco; Morais, Flavia Villaça; Velho, Nádia Maria Rodrigues de Campos
The use of planarians as experimental models has been increased worldwide especially due to their high capacity of tissue regeneration and ease of laboratory maintenance. The current study aims to assess the genetic damage of Girardia tigrina after exposure of stressors in conditions such as: critical temperature, critical pH, hypergravity (4.4G) and low power laser (685nm). 18 specimens of G. tigrina were used, the extreme conditions mentioned were experimental group with 3 specimens each, and 3 for the control group, all specimens were submitted to the Comet Assay after exposure of stressors, and control group with no extreme condition exposure. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation, caused genetic damages to the G. tigrina, for hypergravity and low-power laser stressors the damage was higher in the pharyngeal and post-pharyngeal regions of the animal with a greatest area of comet tail dragging for the post-pharyngeal region. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation caused genetic damages in planarians Brazilian Journal of Development ISSN: 2525-8761 12160 Brazilian Journal of Development, Curitiba, v.8, n.2, p.12159-12177 feb. 2022. G. tigrina, especially in samples from the pharyngeal and post-pharyngeal regions of the flatworms body assessed by the Comet Assay.
Photodynamic effect of protoporphyrin IX in gliosarcoma 9l/lacZ cell line
(Elsevier B.V.) Fontana, Letícia Corrêa; Pinto, Juliana Guerra; Vitorio, Gabrielle dos Santos; Ferreira, Isabelle; Pacheco-Soares, Cristina; Mamone, Leandro; Ferreira‐Strixino, Juliana
Photodynamic Therapy (PDT) is an oncologic treatment, producing reactive oxygen species (ROS) to induce the death of cancer cells. This study aimed to evaluate the action of PDT on gliosarcoma cells, using protoporphyrin IX as PS by incubation with the precursor aminolevulinic acid (ALA). An LED device was used with a light dose of 10 J/cm². The success of the therapy proved to be dependent on the concentration of ALA, and an incubation time of 4 h required for an effective response. Cell death was prevalent due to necrosis when assessed 18 h post-PDT. ALA proved to be an option to PDT in cells of the 9 L/lacZ, with the protocol tested.
(MeOPhSe)2, a synthetic organic selenium compound, inhibits virulence factors of Candida krusei: Adherence to cervical epithelial cells and biofilm formation
(Elsevier GmbH.) Siqueira, Victor Mendes de; Silva, Bruna Graziele Marques da; Passos, Juliene Cristina da Silva; Pinto, Ana Paula; Rocha, João Batista Teixeira da; Silva, Carlos Alberto; Costa, Maricília Silva
Background: Systemic candidiasis is produced by Candida albicans or non-albicans Candida species, opportunistic fungi that produce both superficial and invasive infections. Despite the availability of a wide range of antifungal agents for the treatment of candidiasis, failure of therapy is observed frequently, which opens new avenues in the field of alternative therapeutic strategies. Methods: The effects of p,p′ -methoxyl-diphenyl diselenide [(MeOPhSe)2], a synthetic organic selenium (organochalcogen) compound, were investigated on virulence factors of C. krusei and compared with its antifungal effects on the virulence factors related to adhesion to cervical epithelial cell surfaces with C. albicans. Results: (MeOPhSe)2, a compound non-toxic in epithelial (HeLa) and fibroblastic (Vero) cells, inhibited the growth in a dose-dependent manner and changed the kinetics parameters of C. krusei and, most importantly, extending the duration of lag phase of growth, inhibiting biofilm formation, and changing the structure of biofilm. Also, (MeOPhSe)2 reduced C. albicans and C. krusei adherence to cervical epithelial cells, an important factor for the early stage of the Candida-host interaction. The reduction was 37.24 ± 2.7 % in C. krusei (p = 0.00153) and 32.84 ± 3.2 % in C. albicans (p = 0.0072) at 20 μM (MeOPhSe)2, and the effect is in a concentration-dependent manner. Surprisingly, the antifungal potential on adhesion was similar between both species, indicating the potential of (MeOPhSe)2 as a promising antifungal drug against different Candida infections. Conclusion: Overall, we demonstrated the potential of (MeOPhSe)2 as an effective antifungal drug against the
virulence factors of Candida species.