Navegando por Autor "Bagnato, Vanderlei Salvador"

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    Enhanced viral inactivation by photodynamic therapy using curcumin and methylene blue: an in vitro study with bacteriophage as a viral mode
    (The Royal Society of Chemistry) Miñán, Alejandro Guillermo; Inada, Natalia Mayumi; Ferreira-Strixino, Juliana; Martins, Layla Farage; Silva, Aline Maria da; Bagnato, Vanderlei Salvador
    The global spread of SARS-CoV-2 highlighted the urgent need for broad-spectrum antiviral strategies. One promising approach is antimicrobial photodynamic therapy (aPDT), which, in the presence of oxygen, suitable photosensitizers and irradiation, generates reactive oxygen species capable of inactivating a broad spectrum of viruses. In this work, in vitro aPDT assays were performed using curcumin (Cur) and methylene blue (MB) as biocompatible photosensitizers, with bacteriophage ZC01 serving as the viral model and irradiation at 450 nm and 660 nm. Bacteriophages are frequently used as robust viral models due to their structural tolerance to photodynamic treatments compared with many mammalian viruses. Under the experimental conditions employed, a high viral load (B109 PFU mL-1) was used to represent a challenging inactivation scenario. The combination of curcumin and methylene blue at fluences of 12.5 and 25 J cm-2 per wavelength produced viral load reductions of 7.01log10 and 9.14log10, respectively. These results indicate that Cur/MB combination can achieve efficient photodynamic viral inactivation under moderate irradiation fluences. This in vitro platform may contribute to the development of photodynamic antiviral strategies and provides a practical system for evaluating potential photosensitizers and irradiation conditions for viral photoinactivation.
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    PCR analysis of the effect of photodynamic therapy on breast tumors
    (CDRR Editors) Ferreira, Isabelle; Silva, Glenda Nicioli da; Ferreira-Strixino, Juliana; Grecco, Clovis; Bagnato, Vanderlei Salvador; Salvadori, Daisy Maria Favero; Pinto, Juliana Guerra; Rocha, Noeme Sousa
    Photodynamic therapy (PDT) is a promising therapeutic modality for treating cancer, including breast tumors. The oxidative damage caused by PDT culminates in cell death, induction of immune response, and the resulting destruction of the tumor. This study aimed to evaluate the gene expression profiling of genes BCL-2, BAX, and HER-2 and their proteins after PDT, associating it with the necrosis caused by this therapy under different fluences. Twenty-eight female rats received a single dose of 7,12-dimethylbenz (a) anthracene (DMBA - 80mg/kg), by gavage, for breast tumor induction. After the tumors grew, the animals were divided into four groups: G1 - control group – untreated breast tumor – and G2, G3, and G4 groups treated with PDT using Photogem@ as photosensitizer and interstitial irradiation, with fluences of 50J/cm, 100J/cm, and 150J/cm, respectively. Samples of tumors were harvested for histological examination by RT-qPCR. The RT-qPCR showed that the gene expression profiling of BCL-2, BAX, and HER-2 was not altered after PDT. Hemorrhagic necrosis and qualitatively greater vascular and cellular damage were observed and correlated positively with the fluence. PDT does not seem to induce the modulation of genes related to apoptosis. The results indicate that the type of cell death stimulated by PDT in breast tumor is necrosis.