Navegando por Autor "Gritsch, Kerstin"

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    Hyaluronic Acid-Bupivacaine Hydrogel System for Temporomandibular Joint disorders: Mechanical Lubrification and Local Anesthesia in a preclinical approach
    (Elsevier) Miranda, Diego Garcia; Ramos, Lucas de Paula; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Godoi, Bruno Henrique; Pacheco-Soares, Cristina; Gritsch, Kerstin; Borges, Alexandre Luiz Souto; Grosgogeat, Brigitte
    Temporomandibular disorders (TMD) are primarily characterized by pain and impaired mobility, affecting 5-12% of the adult population and significantly compromising quality of life. Current therapeutic management is multimodal but suffers from high failure rates, highlighting the need for improved interventions. We hypothesized the combination of hyaluronic acid (HA) with bupivacaine (BUP) in the search for a cytocompatibility hydrogel with improved physicochemical properties, suitable for intra-articular therapy of TMD, aiming at physical action, with the viscosity of HA and the prolonged analgesic effects of BUP in a single platform. FTIR analysis confirmed the presence of functional groups of both components. TGA revealed that the HA matrix significantly increased the thermal stability of BUP, delaying its decomposition. Rheological tests demonstrated predominant viscoelastic behavior (G' > G''), with adjustable stiffness depending on the concentration of BUP. Cytocompatibility assays on RAW 264.7 cells (OECD 129) showed that the HA-BUP formulation-maintained cell viability above 98%, in contrast to the use of the anesthetic (BUP) alone (53.6%), and did not exhibit genotoxicity (OECD 487). These findings conclude that the HA-BUP hydrogel has adequate biomechanical, thermal, and biocompatible properties in the laboratory setting. In vivo and clinical studies are needed to evaluate long-term efficacy and safety
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    Ketoprofen Associated with Hyaluronic Acid Hydrogel for Temporomandibular Disorder Treatment: An In Vitro Study
    (MDPI) Miranda, Diego Garcia; Ramos, Lucas de Paula; Lopes, Nicole Fernanda dos Santos; Silva, Nicole Van Der Heijde Fernandes; Soares, Cristina Pacheco; Rodrigues, Flavia Pires; Morais, Vinicius de Paula; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Vasconcellos, Luana Marotta Reis de; Borges, Alexandre Luiz Souto; Grosgogeat, Brigitte; Gritsch, Kerstin
    Temporomandibular disorders (TMD) are a public health problem that affects around 12% of the global population. The treatment is based on analgesics, non-steroidal anti-inflammatory, corticosteroids, anticonvulsants, or arthrocentesis associated with hyaluronic acid-based viscosup- plementation. However, the use of hyaluronic acid alone in viscosupplementation does not seem to be enough to regulate the intra-articular inflammatory process. So, we propose to develop and evaluate the physicochemical and biological properties in vitro of hyaluronic acid hydrogels (HA) associated with ketoprofen (KET) as a new therapeutic treatment for TMD. The hydrogels were synthesized with 3% HA and 0.125, 0.250, 0.500, or 1% KET. Physicochemical analyses of Attenu- ated Total reflectance-Fourier transform infrared spectroscopy (FTIR), Thermogravimetry (TGA), Rheology by Frequency, Amplitude sweeps, temperature ramp, and scanning electron microscopy (SEM) were performed with or without sterilization and cycled. Cytocompatibility and genotoxicity (micronucleus assay) were performed in mouse macrophages (RAW 264-7) for 24 h. Results: FTIR spectrum showed characteristic absorptions of HA and KET. In the TGA, two mass loss peaks were observed, the first representing the water evaporation at 30 and 100 ◦C, and the second peaks be- tween 200 and 300 ◦C, indicating the degradation of HA and KET. Rheology tests in the oscillatory regime classified the hydrogels as non-Newtonian fluids, time-dependent, and thixotropic. Mouse macrophages (RAW 264-7) presented viability of 83.6% for HA, 50.7% for KET, and 92.4%, 66.1%, 65.3%, and 87.7% for hydrogels, in addition to the absence of genotoxicity. Conclusions: Hyaluronic acid associated with ketoprofen shows satisfactory physicochemical and biological properties for use as viscosupplementation. As a limiting point of this study, further research is needed to evaluate the pharmacodynamic, toxicological, and pharmacokinetic characteristics of a complete organism.