Navegando por Autor "Lima, Leonardo Borges de"
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Item Histological analysis of Spinal Cord Injury treated with Amniotic Membrane(Universidade de São Paulo) Correia, Débora Campos Chaves; Lima, Leonardo Borges de; Sant'Anna, Luciana Barros; Lima, Mario Oliveira; Arisawa, Emilia Angela Lo SchiavoSpinal cord injury (SCI) is one of the most harmful syndromes that affects humans due to neuronal destruction and interruption of the nerve impulse transmission between axons. The conduction of motor, sensory, and autonomic responses below the level of the injury is seriously compromised, generating high treatment costs for the health system and a reduction in quality of life, stimulating research into new treatment protocols. This study aimed to evaluate the effectiveness of a biomaterial, the amniotic membrane (AM), to treat experimentally induced SCI. 15 adult rats were divided into three groups (n = 5): S (Sham), L (SCI without treatment), and AM (SCI treated with AM). Spinal cord injury was induced in the region T9-T10 by direct trauma, free-falling a weight (10 g, 2 mm flat edge) held on a mini guillotine, 25 mm above the exposed spinal cord. A fragment of AM, obtained from the human placenta after maternal consent, was applied to the injured area only in the AM group. After 28 days, specimens from the area of spinal cord injury were excised and subjected to routine histological procedures. Data from the semi-quantitative score, obtained from a scheme that assigned different scores to regions of the spinal cord, and from the quantitative analysis were subjected to parametric statistical analysis. Results showed that Group S presented medullary tissue without changes (score 0). In contrast, Group L presented numerous areas of cavitation in the dorsal and lateral regions of the white and gray matter (9.61 ± 6.60 p<0.001) with an intense inflammatory infiltrate. The AM group exhibited small areas of cavitation in the dorsal and lateral regions of the white matter and part of the dorsal columns in the gray matter (0.94 ± 1,03, p<0.001), with few inflammatory cells. The results suggest the effectiveness of AM in the treatment of induced SCI, characterized by a reduction in the evolution of inflammatory and degenerative processes in the central nervous tissue compared to the untreated group.Item Kinematic and sensory-motor analysis of the effects of treatments with photobiomodulation in rats with experimentally induced spinal injury(Springer Nature Link) Beloni, Luís Filipe Karatanasov; Lima, Leonardo Borges de; Correia, Débora Campos Chaves; Mendes, Alessandro Corrêa; Sannt'Anna, Luciana Barros; Arisawa, Emilia Angela Lo Schiavo; Lima, Fernanda Pupio Silva; Lima, Mario OliveiraThe aim of this study was to evaluate the kinematic and sensorimotor parameters after application of photobiomodula- tion (PBM) in rats with experimentally induced spinal cord injuries (SCI). The animals were randomly divided into 3 experimental groups containing 5 animals each and respecting the following arrangement: C (control, simulation of sur- gical procedures); I (injury, induction of SCI) and PBM (photobiomodulation, SCI+application of photobiomodulation therapy). All animals underwent movement tests after 3, 7, 10 and 14 days, respectively, after the surgical procedure, and the following movement parameters were evaluated: mean velocity (MV), ground reaction force (GRF) and sciatic func- tional index (SFI) using integrated equipment capable of digitally collecting the proposed data. The mean results of the MV data obtained by groups C, I and PBM were compared and a statistically significant difference was found, being: C x I (p=0.001) and I x PBM (p=0.002), respectively. Considering the SFI data obtained by groups C, I and PBM, a statisti- cally significant difference was observed when comparing C x I (p = 0.011). The mean results of the GRF data obtained by groups C, I and PBM were compared and the results showed a statistically significant difference in C x I (p=0.001), C x PBM (p=0.026) and I x PBM (p=0.002), respectively. Therefore, there was an improvement in motor function between the 7th and 14th day after SCI, represented by the increase in speed and better GRF distribution in the PBM group.Item Therapeutic Potential of Photobiomodulation in Early Recovery After Experimental Spinal Cord Injury in Rats: Histological and Biomechanical Analysis(Wiley) Correia, Débora Campos Chaves; Lima, Leonardo Borges de; Lima, Mário Oliveira; Beloni, Luís Filipe Karatanasov; Hage, Raduan; Arisawa, Emilia Angela Lo SchiavoSpinal cord injury (SCI) leads to severe functional deficits, underscoring the critical need for new therapies. This study evaluated the efficacy of photobiomodulation (PBM) as an early, noninvasive treatment for induced SCI, using specific parameters (808 nm, 72J/cm2, 100mW). A total of 15 rats were divided into Control (C), SCI, and PBM groups. Efficacy was determined by an inte- grated approach, correlating ground reaction force (GRF) with quantitative histological assessment. The PBM group showed a significant reduction of secondary damage (33.3 ± 5.5 vs. 53.9 ± 7.0 in the SCI group; p = 0.0002) and preserved neural structure. This tissue preservation aligns with the GRF analysis, which demonstrated that the PBM group recovered gait patterns similar to the Control group. In conclusion, PBM effectively mitigates necrosis aerea, maintaining tissue integrity, improving functional recovery, and reinforcing the PBM's therapeutic potential as a promising translatable strategy for outcomes after SCI. Further research should include immunostaining of cells and larger samples.