Navegando por Autor "Silva, Nicole Van Der Heijde Fernandes"

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    Exploring Antioxidant, Antimicrobial and Anti-Inflammatory Effects of Juglans regia and Pfaffia paniculata Extracts: implications for Intestinal Dysbiosis and Colorectal Cancer Risk Associated with Oral Pathogens
    (MDPI) Miranda, Diego Garcia; Ramos, Lucas de Paula; Attik, Nina; Silva, Nicole Van Der Heijde Fernandes; Camargo, Pyetra Claro; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Marcucci, Maria Cristina; Soares, Cristina Pacheco; Godoi, Bruno Henrique; Caires, Giovanna Arruda; Vigerelli, Hugo; Carrouel, Florence
    Colorectal neoplasms rank as the third most prevalent cancer globally and stand as the second leading cause of cancer-related mortality. Its etiol- ogy is multifaceted, pointing to the role of microorganisms within the human microbiota in its development. Notably, the high prevalence of oral pathogens like Fusobacterium nucleatum and Parvimonas micra is implicated in inducing gut dysbiosis and stimulating the proliferation and metastasis of cancer cells. Therefore, this study aimed to evaluate in vitro the biological effects of extracts from Juglans regia and Pfaffia paniculata. Methods: Phytochemical analysis was carried out by HPLC, and the antioxidant effect was deter- mined by DPPH. Antimicrobial activity was investigated on F. nucleatum and P. micra planktonic and biofilms. Metabolic activity and genotoxicity were performed. Results: J. regia and P. paniculata expressed CE50 37.26 and 1367.57 mcg, respectively. The extracts exhibited a minimum bactericidal concentration of 1.73 and 0.48 mg/mL for J. regia and P. paniculata, respectively. Reduction superiorly 90% of P. micra biofilms. Metabolic activity was varied proportionally to the extract concentration, and no genotoxic effects were ob- served. Conclusions: The J. regia extract has great antioxidant activity and could be used as an alternative in combating pathogens associated with the onset of dysbiosis and tumor progression in colorectal neoplasms. Nevertheless, further studies are needed to validate their clinical applicability.
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    Ketoprofen Associated with Hyaluronic Acid Hydrogel for Temporomandibular Disorder Treatment: An In Vitro Study
    (MDPI) Miranda, Diego Garcia; Ramos, Lucas de Paula; Lopes, Nicole Fernanda dos Santos; Silva, Nicole Van Der Heijde Fernandes; Soares, Cristina Pacheco; Rodrigues, Flavia Pires; Morais, Vinicius de Paula; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Vasconcellos, Luana Marotta Reis de; Borges, Alexandre Luiz Souto; Grosgogeat, Brigitte; Gritsch, Kerstin
    Temporomandibular disorders (TMD) are a public health problem that affects around 12% of the global population. The treatment is based on analgesics, non-steroidal anti-inflammatory, corticosteroids, anticonvulsants, or arthrocentesis associated with hyaluronic acid-based viscosup- plementation. However, the use of hyaluronic acid alone in viscosupplementation does not seem to be enough to regulate the intra-articular inflammatory process. So, we propose to develop and evaluate the physicochemical and biological properties in vitro of hyaluronic acid hydrogels (HA) associated with ketoprofen (KET) as a new therapeutic treatment for TMD. The hydrogels were synthesized with 3% HA and 0.125, 0.250, 0.500, or 1% KET. Physicochemical analyses of Attenu- ated Total reflectance-Fourier transform infrared spectroscopy (FTIR), Thermogravimetry (TGA), Rheology by Frequency, Amplitude sweeps, temperature ramp, and scanning electron microscopy (SEM) were performed with or without sterilization and cycled. Cytocompatibility and genotoxicity (micronucleus assay) were performed in mouse macrophages (RAW 264-7) for 24 h. Results: FTIR spectrum showed characteristic absorptions of HA and KET. In the TGA, two mass loss peaks were observed, the first representing the water evaporation at 30 and 100 ◦C, and the second peaks be- tween 200 and 300 ◦C, indicating the degradation of HA and KET. Rheology tests in the oscillatory regime classified the hydrogels as non-Newtonian fluids, time-dependent, and thixotropic. Mouse macrophages (RAW 264-7) presented viability of 83.6% for HA, 50.7% for KET, and 92.4%, 66.1%, 65.3%, and 87.7% for hydrogels, in addition to the absence of genotoxicity. Conclusions: Hyaluronic acid associated with ketoprofen shows satisfactory physicochemical and biological properties for use as viscosupplementation. As a limiting point of this study, further research is needed to evaluate the pharmacodynamic, toxicological, and pharmacokinetic characteristics of a complete organism.