Navegando por Autor "Strixino, Juliana Ferreira"

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    Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
    (MDPI) Souza, Beatriz Müller Nunes; Pinto, Juliana Guerra; Pereira, André Henrique Correia; Miñán, Alejandro Guillermo; Strixino, Juliana Ferreira
    Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
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    Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes
    (MDPI) Pereira, André Henrique Correia; Marcolino, Luciana Maria Cortez; Pinto, Juliana Guerra; Strixino, Juliana Ferreira
    Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 μg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 μg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed.
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    Photobiomodulation as a strategy to enhance argan oil permeation across diverse hair types
    (Elsevier) Susigan, Bianca Felippe; André, Luana Prado; Lyra, Lucas Ferreira; Sakane, Kumiko Koibuchi; Pinto, Juliana Guerra; Strixino, Juliana Ferreira
    Photobiomodulation (PBM), which employs low-intensity laser light, stimulates biological processes, promoting hair growth, thickness, and regeneration without thermal damage. This study aimed to evaluate and compare the impact of photobiomodulation using amber LED combined with infrared laser on the permeation of argan oil in different types of hair fibers. The research involved samples of African and Asian hair treated with PBM and argan oil, divided into control and experimental groups. The analysis included confocal fluorescence microscopy, scanning electron microscopy (SEM), and FTIR-ATR spectroscopy, revealing significant improvements in cuticle sealing and oil permeation, particularly in Asian hair. African fibers, while showing less sealing, also exhibited notable structural changes. The infrared spectra of the samples processed using PBM with argan oil showed statistically significant differences in the regions of the Amide A, I, and II bands, which are related to the proteins in the samples. The combination of PBM with argan oil proved promising for reconditioning and protecting hair fibers, with potential applications in the cosmetic industry to meet diverse hair care needs.