Navegando por Assunto "Hyaluronic acid"

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    Hyaluronic Acid-Bupivacaine Hydrogel System for Temporomandibular Joint disorders: Mechanical Lubrification and Local Anesthesia in a preclinical approach
    (Elsevier) Miranda, Diego Garcia; Ramos, Lucas de Paula; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Godoi, Bruno Henrique; Pacheco-Soares, Cristina; Gritsch, Kerstin; Borges, Alexandre Luiz Souto; Grosgogeat, Brigitte
    Temporomandibular disorders (TMD) are primarily characterized by pain and impaired mobility, affecting 5-12% of the adult population and significantly compromising quality of life. Current therapeutic management is multimodal but suffers from high failure rates, highlighting the need for improved interventions. We hypothesized the combination of hyaluronic acid (HA) with bupivacaine (BUP) in the search for a cytocompatibility hydrogel with improved physicochemical properties, suitable for intra-articular therapy of TMD, aiming at physical action, with the viscosity of HA and the prolonged analgesic effects of BUP in a single platform. FTIR analysis confirmed the presence of functional groups of both components. TGA revealed that the HA matrix significantly increased the thermal stability of BUP, delaying its decomposition. Rheological tests demonstrated predominant viscoelastic behavior (G' > G''), with adjustable stiffness depending on the concentration of BUP. Cytocompatibility assays on RAW 264.7 cells (OECD 129) showed that the HA-BUP formulation-maintained cell viability above 98%, in contrast to the use of the anesthetic (BUP) alone (53.6%), and did not exhibit genotoxicity (OECD 487). These findings conclude that the HA-BUP hydrogel has adequate biomechanical, thermal, and biocompatible properties in the laboratory setting. In vivo and clinical studies are needed to evaluate long-term efficacy and safety