Navegando por Assunto "Hydrogels"

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    3D bioprinted human iPSC-derived neural progenitor cells as a novel platform for studying neurogenic niche
    (AIP Publishing) Machado, Lucas Simões; Ferreira, Paula Scanavez; Pires, Marina Rodrigues; Bim, Larissa Valdemarin; Oliveira, Natália Heloísa de; Salles, Geisa Rodrigues; Ferreira, Natalia Dall'Agnol; Cruz, Elisa Marozzi; Porcionatto, Marimelia Aparecida
    Animal models, especially rodents, used to study neurodevelopment have significantly advanced our comprehension of cellular and molecular mechanisms. Nevertheless, differences in species-specific structures, gestation periods, and interneuronal connections limit animal models’ ability to represent human neurodevelopment accurately. The unique characteristics of primate neural progenitor cells (NPCs) enable cortex expansion with gyrus formation, which does not occur in lissencephalic animals, like rodents. Therefore, there is a need for novel in vitro models using human cells that recapitulate the complexity of human brain development. Along with organoids, 3D bioprinting offers a platform for creating more complex in vitro models. We developed, extensively characterized, and successfully used a GeltrexTM/GelMA hydrogel blend to bioprint human induced pluripotent stem cells-derived NPCs (hNPCs). We show that 3D bioprinted hNPCs can selforganize, revealing key features of a neurogenic niche, including proliferation, differentiation, and migration, remaining viable for over 110 days. Within the first 20 days, bioprinted constructs showed the formation of positive cell clusters for the neurogenic niche cell markers FABP7, NESTIN, and GFAP. Clusters were interconnected by process bundles supporting cell migration. The cells proliferated within the clusters, and over time, NPCs originated TUBB3þ neurons with long axonal tracts, prominent around the clusters. We propose this as a 4D model to study neurogenic niches’ key cellular and molecular features in a 3D bioprinted scaffold, adding time as the fourth dimension. Neuronal maturation in this dynamic model recapitulates key neurogenic niche properties, making it suitable for neurodevelopmental disease modeling and drug screening.
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    Hyaluronic Acid-Bupivacaine Hydrogel System for Temporomandibular Joint disorders: Mechanical Lubrification and Local Anesthesia in a preclinical approach
    (Elsevier) Miranda, Diego Garcia; Ramos, Lucas de Paula; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Godoi, Bruno Henrique; Pacheco-Soares, Cristina; Gritsch, Kerstin; Borges, Alexandre Luiz Souto; Grosgogeat, Brigitte
    Temporomandibular disorders (TMD) are primarily characterized by pain and impaired mobility, affecting 5-12% of the adult population and significantly compromising quality of life. Current therapeutic management is multimodal but suffers from high failure rates, highlighting the need for improved interventions. We hypothesized the combination of hyaluronic acid (HA) with bupivacaine (BUP) in the search for a cytocompatibility hydrogel with improved physicochemical properties, suitable for intra-articular therapy of TMD, aiming at physical action, with the viscosity of HA and the prolonged analgesic effects of BUP in a single platform. FTIR analysis confirmed the presence of functional groups of both components. TGA revealed that the HA matrix significantly increased the thermal stability of BUP, delaying its decomposition. Rheological tests demonstrated predominant viscoelastic behavior (G' > G''), with adjustable stiffness depending on the concentration of BUP. Cytocompatibility assays on RAW 264.7 cells (OECD 129) showed that the HA-BUP formulation-maintained cell viability above 98%, in contrast to the use of the anesthetic (BUP) alone (53.6%), and did not exhibit genotoxicity (OECD 487). These findings conclude that the HA-BUP hydrogel has adequate biomechanical, thermal, and biocompatible properties in the laboratory setting. In vivo and clinical studies are needed to evaluate long-term efficacy and safety