Navegando por Assunto "Osteogenesis"

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    Calcium aluminate cement blended to bioactive glass and strontium: in vitro and in vivo evaluation studies
    (Springer Nature) Gonçalves, Isabela dos Santos; Donda, Giovanni Moreira; Oliveira Filho, Hugo Gutemberg Patino de; Fernandes, Marina Santos; Barbosa, Ana Maria; Raniero, Leandro José; Vasconcellos, Luana Marotta Reis de; Oliveira, Ivone Regina de
    Homogeneous calcium aluminate cement–based (CH) materials hold potential as biomaterials, while bioactive glass (BG-58S) is a promising bone regeneration material with excellent bioactivity. Strontium, known for stimulating bone tissue regeneration, was incorporated into CH-BG-58S blends to enhance bioactivity, osteogenesis, and bone tissue repair. Methods Blends with only BG-58S were evaluated for viscosity, injectability, and workability. In vitro tests were performed using MG63 cells and in vivo tests utilized blends CH-7.5BG-58S and CH-7.5BG-58S/Sr to fill monocortical critical bone defects. Bactericidal capacity against Escherichia coli and Staphylococcus aureus was also evaluated. The addition of BG to CH increased viscosity, decreased injectability, and improved workability. Results All blends were non-cytotoxic, and CH-7.5BG-58S and CH-10BG-58S blends exhibited bactericidal capacity against E. coli. Osteogenic activity, confirmed by mineralization nodule formation, was observed in all blends. Histomorphometric and histological analyses revealed bone neoformation, with emphasis on SrO-containing groups, but without statistical significance among groups (p > 0.05). Conclusion This research highlights the potential of CH-BG-58S blends, particularly those with strontium, as bioactive materials for bone tissue engineering.
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    Incorporation of calcium aluminate and zirconia in polymethylmethacrylate (PMMA) bone cement for biomedical applications: study of in vitro osteogenesis
    (Universidade Estadual Paulista) Lupp, Juliana Dos Santos; Silva, Luis Augusto de Almeida; Ramos, Nathalia de Souza; Oliveira, Ivone Regina de; Oliveira, Felipe Eduardo de; Avelino, Sarah de Oliveira Marco; Gonçalves, Isabela dos Santos; Vaconcellos, Luana Marotta Reis de
    Objective: To evaluate the effects of incorporating calcium aluminate cement (CAC) and monoclinic zirconia (Z) into polymethylmethacrylate (PMMA) bone cement on in vitro osteogenesis. Material and Methods: Samples of pure PMMA, PMMA+CAC 7.5% (wt%) and PMMA+Z 7.5% (wt%) were prepared. The formulations were characterized by scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, and wettability analysis. For biological assessment, mesenchymal stem cells derived from Wistar rat femurs were cultured on the samples. Cellular response was analyzed by cell viability assay, protein synthesis, alkaline phosphatase (ALP) activity, tumor necrosis factor-alpha (TNF-α) expression by ELISA, and mineralized nodule formation. Results: All groups exhibited cell viability greater than 70% (p>0.05). ALP activity and protein synthesis showed no significant differences (p>0.05). TNF-α expression was significantly higher in the control group (41.25 ± 17.00 pg/mL) compared with PMMA (22.97 ± 7.08; p<0.01), PMMA+Z (25.76 ± 13.08; p<0.05), and PMMA+CAC (28.99 ± 14.71; p<0.05), suggesting inflammatory modulation. All groups presented mineralized nodules. In the wettability analysis, a numerical reduction in the contact angle was observed for PMMA+Z compared with pure PMMA, but without statistical significance (p>0.05). Conclusion: The addition of CAC and Z to PMMA resulted in more homogeneous surfaces, a trend toward increased wettability, and reduced TNF-α expression, without impairing cell viability or osteogenic potential, highlighting the promising potential of these formulations for bone regeneration.