Navegando por Assunto "Oxidative stress"

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    In vitro evaluation of red and near infrared LED photobiomodulation on L929 fibroblasts metabolic activity and morphology
    (Springer) Segismondi, Larissa Cavallieri; Soares, Luís Eduardo Silva; Pacheco-Soares, Cristina
    Purpose Fibroblasts, the main cells of connective tissue, are highly susceptible to oxidative stress caused by reactive oxygen species (ROS). Photobiomodulation (PBM) has emerged as a promising therapy capable of modulating biological tissues, enhancing cellular metabolic activity, and promoting the proliferation and survival of fibroblasts. In this study, we aimed to investigate the potential in vitro photoprotective effect of cellular photomodulation using 660 and 850 nm LEDs in L929 fibroblast cells treated with hydrogen peroxide as a model of oxidative stress. Methods Changes in cell viability were observed using the Alamar Blue colorimetric assay, and cell morphology was assessed by inverted microscopy. Mitochondria and nuclei were also labeled in living cells using fluorescence with TMRM and Hoechst, in addition to ROS detection with CellRox Green. Results Our results indicate that pretreatment with LED exerts a cytoprotective effect against oxidative stress, promoting an increase in mitochondrial activity, mitochondrial membrane potential, and a reduction in intracellular reactive oxygen species (ROS) generation while inducing improvements in the morphological characteristics of cells. Conclusion The findings from the present study indicate that Photobiomodulation (PBM) with LED contributes to maintaining cellular homeostasis and can help prevent and mitigate damage resulting from oxidative stress in fibroblasts.
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    In vitro evaluation of red and near infrared LED photobiomodulation on L929 fibroblasts metabolic activity and morphology
    (Springer) Segismondi, Larissa Cavallieri; Soares, Luís Eduardo Silva; Soares, Cristina Pacheco
    Purpose Fibroblasts, the main cells of connective tissue, are highly susceptible to oxidative stress caused by reactive oxygen species (ROS). Photobiomodulation (PBM) has emerged as a promising therapy capable of modulating biological tissues, enhancing cellular metabolic activity, and promoting the proliferation and survival of fibroblasts. In this study, we aimed to investigate the potential in vitro photoprotective effect of cellular photomodulation using 660 and 850 nm LEDs in L929 fibroblast cells treated with hydrogen peroxide as a model of oxidative stress. Methods Changes in cell viability were observed using the Alamar Blue colorimetric assay, and cell morphology was assessed by inverted microscopy. Mitochondria and nuclei were also labeled in living cells using fluorescence with TMRM and Hoechst, in addition to ROS detection with CellRox Green. Results Our results indicate that pretreatment with LED exerts a cytoprotective effect against oxidative stress, promoting an increase in mitochondrial activity, mitochondrial membrane potential, and a reduction in intracellular reactive oxygen spe- cies (ROS) generation while inducing improvements in the morphological characteristics of cells. Conclusion The findings from the present study indicate that Photobiomodulation (PBM) with LED contributes to maintain- ing cellular homeostasis and can help prevent and mitigate damage resulting from oxidative stress in fibroblasts.
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    Photobiomodulation by LED 660 nm and Taurine against H2O2 oxidative stress in SH-SY5Y cells
    (Springer Nature Link) Rossato, Rafaella Carvalho; Salles, Geisa Rodrigues; Albuquerque, Amanda Lira; Porcionatto, Marimélia Aparecida; Granato, Alessandro Eustáquio Campos; Ulrich, Henning; Santos, Mariela Inês Batista dos; Soares, Cristina Pacheco
    Alzheimer's Disease (AD) is a progressive uncurable neurodegenerative pathology affecting millions worldwide. Photo- biomodulation and Taurine are promising alternatives for preventing and reducing the rapid progression of neurodegenera- tion, stimulating the reconstructing of neural tissue structures, especially improving mitochondrial activity, which is highly impaired in AD. In this study, the mitochondrial effects of Taurine combined with light emitting diode (LED) irradiation were evaluated on human neuroblastoma cells (SH-SY5Y), under oxidative stress condition by hydrogen peroxide (H2O2) exposure, a considerable modulator in AD. We evaluated LED irradiation at the wavelength of 660 nm and Taurine under different concentrations before and together with exposing SH-SY5Y cells to different concentrations of H2O2, assessing mitochondrial activity by the MTT colorimetric test and labeling live cells mitochondria by the fluorescent probe MitoTracker. Cell viability was also evaluated by the trypan blue exclusion assay, and cellular morphological structures were imaged by scanning electron microscopy (SEM). Neuroprotective effects were achieved by both LED irradiation and LED irradia- tion + Taurine when cells were exposed to them before H2O2-induced stress. Comparing both agents, LED irradiation at 660 nm is sufficient to improve mitochondrial activity, however, healthy mitochondrial morphology was only observed when cells were treated with Taurine together with LED irradiation, representing affordable candidates that act in synergy against oxidative stress, one of the main contributors to neurodegeneration.
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    Small Structural Differences in Proline-Rich Decapeptides Have Specific Effects on Oxidative Stress-Induced Neurotoxicity and L-Arginine Generation by Arginosuccinate Synthase
    (MDPI) Silva, Carlos Alberto; Silva, Brenda Rufino; Silva, Julio Cezar Araujo; Silva, Felipe Assumpção da Cunha e; Kodama, Roberto Tadashi; Silva, Wilmar Dias da; Costa, Maricilia Silva; Portaro, Fernanda Calheta Vieira
    ntroduction. The proline-rich decapeptide 10c (Bj-PRO-10c; ENWPHPQIPP) from the Bothrops jararaca snake modulates argininosuccinate synthetase (AsS) activity to stimulate L-arginine metabolite production and neuroprotection in the SH-SY5Y cell line. The relationships between structure, interactions with AsS, and neuroprotection are little known. We evaluated the neuro- protective effects of Bj-PRO-10c and three other PROs (Bn-PRO-10a, Bn-PRO-10c > Bn-PRO-10a-MK > Bn-PRO-10a. The structure of PROs and their correlations with enzyme activity revealed that histidine (H5) and glutamine (Q7) in Bj-PRO-10c potentiated their affinity for AsS. Conclusions. Our investigation provides the first insights into the structure and molecular interactions of PROs with AsS, which could possibly further their neuropharmacological applications.