Produção acadêmica-PPGPM

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Navegando Produção acadêmica-PPGPM por Assunto "Acrylic acid"

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    Gelatin nanoparticles via template polymerization for drug delivery system to photoprocess application in cells
    (Taylor & Francis) Trindade, Agnes Cecheto; Castro, Pedro Augusto Rodrigues Ribeiro de; Pinto, Bruna Cristina dos Santos; Ambrósio, Jéssica Aparecida Ribeiro; Oliveira Junior, Benedito Marcio de; Beltrame Junior, Milton; Gonçalves, Erika Peterson; Pinto, Juliana Guerra; Ferreira-Strixino, Juliana; Simioni, Andreza Ribeiro
    Photodynamic therapy (PDT) is a clinical treatment based on the activation of light-absorbing photosensitizers (PS) to generate reactive oxygen species, which are toxic to the targeted disease cells. Because most PS are hydrophobic with poor water solubility, it is necessary to encapsulate and solubilize PS in aqueous condi- tions to improve the photodynamic action for this compound. In this work, gelatin-poly(acrylic acid) nanoparticles (PAA/gelatin nanoparticles) via template polymerization for incorporation alu- minum chloride phthalocyanine (ClAlPc) as a model drug for PDT application were developed. Biocompatible core-shell polymeric nanoparticles were fabricated via template polymerization using gelatin and acrylic acid as a reaction system. The nanoparticulate system was studied by scanning electron microscopy, steady- state, and their biological activity was evaluated using in vitro cancer cell lines by classical MTT assay. The obtained nanopar- ticles had a spherical shape and DLS particle size were deter- mined further and was found to be around 170nm. The phthalocyanine-loaded-nanoparticles maintained their photophysi- cal behaviour after encapsulation. It is found that ClAlPc can be released from the nanoparticles in a sustained manner with a small initial burst release. In vitro cytotoxicity revealed that ClAlPc- loaded nanoparticles had similar cytotoxicity to free ClAlPc with mouse melanoma cancer cell line (B16-F10). In vitro photoeffects assay indicated that the nanoparticle formulation was superior in anticancer effect to free ClAlPc on mouse melanoma cancer cell line B16-F10. The results indicate that ClAlPc encapsulated in gel- atin-poly(acrylic acid) nanoparticles are a successful delivery sys- tem for improving photodynamic activity in the target tissue.