Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications

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dc.contributor.authorToledo, Maria Cristina Modesto Clementino de
dc.contributor.authorAbreu, Alexandro da Silva
dc.contributor.authorCarvalho, Janicy Arantes
dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorGodoy, Daniele da Silva
dc.contributor.authorPinto, Bruna Cristina dos Santos
dc.contributor.authorBeltrame Junior, Milton
dc.contributor.authorSimioni, Andreza Ribeiro
dc.date.accessioned2023-09-26T14:50:34Z
dc.date.available2023-09-26T14:50:34Z
dc.date.issued22020-08-21
dc.description.abstractBackground: Photodynamic Therapy (PDT) is a modality for the treatment of neoplastic tissues, which is based on the administration of a phototherapeutic agent and light irradiation at an appropriate wavelength, aiming to locate and destroy the target cell with the formation of reactive oxygen species. Nanoencapsulation technology presents itself as a tool for incorporation of bioactive substances aiming to improve their solubility in physiological environment, obtain a longer circulation time in the organism, administration of lower dosages and the minimization of side effects. The present work aimed at the development of poly (lactic acid-glycolic acid) (PLGA) nanoparticles coated with polyelectrolyte film layers for encapsulating zinc phthalocyanine tetrasulfonated (ZnPcSO4) as a bioactive substance model. Methods: PLGA nanoparticles were produced by the double emulsion/solvent evaporation technique and polyelectrolytic coating was performed using polyalkylamine hydrochloride (PAH) as a weak polycation and poly (4- styrene sulfonate) (PSS) as a strong polyanion by layer-by-layer self-assembly technique (known as layer-by-layer-LbL). The nanoparticulate system was studied by scanning electron microscopy, steady-state, and their biological activity was evaluated using in vitro cancer cell lines by classical MTT assay. Results: The polyelectrolytic PLGA nanoparticles had an average diameter of 384.7 ± 138.6 nm, restricted distribution size with a polydispersity index. The obvious change in zeta potential indicates successful alternation in polycation (PAH) and polyanion (PSS) deposition directly in PLGA nanoparticles. Scanning electron microscopy (SEM) analysis showed that the formed system had morphology spherical, typical of these release systems. The loading efficiency was 82.1 % ± 1.2 %. The polyelectrolytic nanoparticles loaded with phthalocyanine maintained their photophysical behavior after encapsulation. Cell viability was determined, obtaining 90 % cell death. Conclusions: Therefore, the presented work depicts ZnPcSO4-loaded polyelectrolytic PLGA nanoparticles as a promise drug delivery system for phototherapeutic agent, which are thus expected to have superior therapeutic efficiency than free drug.pt_BR
dc.description.physical10 f.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.uriProjeto numero 2018/18531-6.pt_BR
dc.format.mimetypepdfpt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.affiliationUniversidade de São Paulopt_BR
dc.identifier.affiliationUniversidade de São Paulopt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.affiliationUniversidade do Vale do Paraíbapt_BR
dc.identifier.bibliographicCitationTOLEDO, M. C. M. C.; ABREU, A. D. S.; CARVALHO, J. A.; AMBRÓSIO, J. A. R.; GODOY, D. S.; PINTO, B. C. S.; BELTRAME JUNIOR, M.; SIMIONI, A. R. Zinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applications. Photodiagnosis and Photodynamic Therapy, v. 32, p. 1-10, 2020. (18/18531-6).https://www.sciencedirect.com/science/article/abs/pii/S1572100020303203pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.pdpdt.2020.101966
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/321
dc.language.isoenpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.holderToledo, Maria Cristina Modesto Clementino dept_BR
dc.rights.holderAbreu, Alexandro da Silvapt_BR
dc.rights.holderCarvalho, Janicy Arantespt_BR
dc.rights.holderAmbrósio, Jéssica Aparecida Ribeiropt_BR
dc.rights.holderGodoy, Daniele da Silvapt_BR
dc.rights.holderPinto, Bruna Cristina dos Santospt_BR
dc.rights.holderBeltrame Junior, Miltonpt_BR
dc.rights.holderSimioni, Andreza Ribeiropt_BR
dc.subject.keywordNanoparticlespt_BR
dc.subject.keywordPhthalocyaninept_BR
dc.subject.keywordPhotodynamic therapypt_BR
dc.subject.keywordPolyelectrolytept_BR
dc.titleZinc phthalocyanine tetrasulfonate-loaded polyelectrolytic PLGA nanoparticles for photodynamic therapy applicationspt_BR
dc.typeArtigos de Periódicospt_BR

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