Synthesis, characterization, and evaluation of chloroaluminium phthalocyanine incorporated in poly(ε-caprolactone) nanoparticles for photodynamic therapy

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dc.contributor.authorPinto, Bruna Cristina dos Santos
dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorMarmo, Vitor Luca Moura
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorRaniero, Leandro José
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorSimioni, Andreza Ribeiro
dc.contributor.authorBeltrame Junior, Milton
dc.date.accessioned2023-09-26T14:14:20Z
dc.date.available2023-09-26T14:14:20Z
dc.date.issued22022-04-05
dc.description.abstractBackground: The use of nanotechnology has been widely used in biomedical science, including orthopedic implants, tissue engineering, cancer therapy and drug elution from nanoparticle systems, such as poly-caprolactone (PCL) nanoparticles, which stand out mainly for their biocompatibility, being considered as effective carriers for photosensitizing drugs (PS) in photodynamic therapy (PDT) protocols. Methods: This manuscript describes the synthesis and characterization of PCL nanoparticles for controlled release of the drug chloro-aluminum phthalocyanine (ClAlPc) as a photosensitizer for application in PDT. The PCL-ClAlPc nanoparticles were developed by the nanoprecipitation process. The structure and morphology of the nanoparticles were studied with scanning electron microscopy (SEM) and with Fourier transform infrared (FTIR). The size of nanomaterials was studied using the Dynamic Light Scattering (DLS) method. Photophysical and photochemical characterizations were performed. Subsequently, photobiological studies were also used to characterize the system. Results: The nanoparticles had an average diameter of 384.7 ± 138.6 nm and a polydispersity index of 0.153. SEM analysis revealed that the system formed a spherical shape typical of these delivery systems. Charging efficiency was 82.1% ± 1.2%. The phthalocyanine-loaded PCL nanoparticles maintained their photophysical behavior after encapsulation. Cell viability was determined after the dark toxicity test, and it was possible to observe that there was no evidence of toxicity in the dark, for all concentrations tested. The assay also revealed that adenocarcinoma cells treated with free ClAlPc and in the nanoformulation showed 100% cell death when subjected to PDT protocols. The intracellular location of the photosensitizer indicated a high potential for accumulation in the cytoplasm and nucleus. Conclusions: From the photophysical, photochemical and photobiological analyzes obtained, it was possible to observe that the development of PCL nanoparticles encapsulated with ClAlPc, by the nanoprecipitation method was adequate and that the in vivo release study is efficient to reduce the release rate and attenuate the burst of PS loaded on PCL nanoparticles. The results reinforce that the use of this system as drug delivery systems is useful in PDT protocols.pt_BR
dc.description.physical9 f.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.uriProjeto numero 2018/18531-6pt_BR
dc.format.mimetypepdfpt_BR
dc.identifier.affiliationUnivappt_BR
dc.identifier.bibliographicCitationPINTO, B. C.; AMBROSIO, J. A.; MARMO, V. L. M.; PINTO, J. G.; RANIERO, L. J.; FERREIRA-STRIXINO, J.; SIMIONI, Andreza R.; BELTRAME JR, M. (2022). Synthesis, characterization, and evaluation of chloroaluminium phthalocyanine incorporated in poly (ε-caprolactone) nanoparticles for photodynamic therapy. Photodiagnosis and Photodynamic Therapy, v. 38, p. 102850, 2022. (18/18531-6).pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.pdpdt.2022.102850
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/320
dc.language.isopt_BRpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.holderPinto, Bruna Cristina dos Santospt_BR
dc.rights.holderAmbrósio, Jéssica Aparecida Ribeiropt_BR
dc.rights.holderMarmo, Vitor Luca Mourapt_BR
dc.rights.holderPinto, Juliana Guerrapt_BR
dc.rights.holderRaniero, Leandro Josépt_BR
dc.rights.holderFerreira-Strixino, Julianapt_BR
dc.rights.holderSimioni, Andreza Ribeiropt_BR
dc.rights.holderBeltrame Junior, Miltonpt_BR
dc.subject.keywordPoly-caprolactonept_BR
dc.subject.keywordChloro-aluminum phthalocyaninept_BR
dc.subject.keywordPhotodynamic therapypt_BR
dc.titleSynthesis, characterization, and evaluation of chloroaluminium phthalocyanine incorporated in poly(ε-caprolactone) nanoparticles for photodynamic therapypt_BR
dc.typeArtigos de Periódicospt_BR

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