Synthesis and characterization of photosensitive gelatin-based hydrogels for photodynamic therapy in HeLa-CCL2 cell line

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dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorPinto, Bruna Cristina dos Santos
dc.contributor.authorMarmo, Vitor Luca Moura
dc.contributor.authorSantos, Kennedy Wallace dos
dc.contributor.authorBeltrame Junior, Milton
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorRaniero, Leandro José
dc.contributor.authorSimioni, Andreza Ribeiro
dc.date.accessioned2023-09-26T00:14:40Z
dc.date.available2023-09-26T00:14:40Z
dc.date.issued22022-03-22
dc.description.abstractBackground: Hydrogel systems are increasingly gaining visibility involving biomedicine, tissue engineering, environmental treatments, and drug delivery systems. These systems have a three-dimensional network composition and high-water absorption capacity, are biocompatible, allowing them to become an option as photosensitizer carriers (PS) for applications in Photodynamic Therapy (PDT) protocols. Methods: A nanohydrogel system (NAHI), encapsulated with chloroaluminium phthalocyanine (ClAlPc) was synthesized for drug delivery.. NAHI was synthesized using gelatin as based polymer by the chemical cross-linking technique. The drug was encapsulated by immersing the hydrogel in a 1.0 mg.mL􀀀 1 ClAlPc solution. The external morphology of NAHI was examined by scanning electron microscopy (SEM). The degree of swelling of the synthesized system was evaluated to determine the water absorption potential. The produced nanohydrogel system was characterized by photochemical, photophysical and photobiologial studies. Results: The images from the SEM analysis showed the presence of three-dimensional networks in the formulation. The swelling test demonstrated that the nanohydrogel freeze-drying process increases its water holding capacity. All spectroscopic results showed excellent photophysical parameters of the drug studied when served in the NAHI system. The incorporation efficiency was 70%. The results of trypan blue exclusion test have shown significant reduction (p < 0.05) in the cell viability for all groups treated with PDT, in all concentrations tested. In HeLa cells, PDT mediated by 0,5 mg.mL􀀀 1 ClAlPc encapsulated in NAHI showed a decrease in survival close to 95%. In the internalization cell study was possible to observe the internalization of phthalocyanine after one hour of incubation, at 37 ◦C, with the the accumulation of PS in the cytoplasm and inside the nucleus at both concentrations tested. Conclusions: Given the peculiar performance of the selected system, the resulting nanohydrogel is a versatile platform and display potential applications as controlled delivery systems of photosensitizer for photodynamic therapy application.pt_BR
dc.description.physical9 f.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.uriProjeto número 2018/18531-6.pt_BR
dc.format.mimetypepdfpt_BR
dc.identifier.affiliationUnivappt_BR
dc.identifier.bibliographicCitationAMBRÓSIO, Jéssica A.R. et al. Synthesis and characterization of photosensitive gelatin-based hydrogels for photodynamic therapy in HeLa-CCL2 cell line. Photodiagnosis and Photodynamic Therapy, v. 38, p. 102818, 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.pdpdt.2022.102818
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/317
dc.language.isopt_BRpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.holderAmbrósio, Jéssica Aparecida Ribeiropt_BR
dc.rights.holderPinto, Bruna Cristina dos Santospt_BR
dc.rights.holderMarmo, Vitor Luca Mourapt_BR
dc.rights.holderSantos, Kennedy Wallace dospt_BR
dc.rights.holderBeltrame Junior, Miltonpt_BR
dc.rights.holderPinto, Juliana Guerrapt_BR
dc.rights.holderFerreira-Strixino, Julianapt_BR
dc.rights.holderRaniero, Leandro Josépt_BR
dc.rights.holderSimioni, Andreza Ribeiropt_BR
dc.subject.keywordChloroaluminium phthalocyaninept_BR
dc.subject.keywordGelatinpt_BR
dc.subject.keywordHydrogelpt_BR
dc.subject.keywordPhotodynamic therapypt_BR
dc.subject.keywordFármacos Fotossensibilizantespt_BR
dc.subject.keywordFotoquimioterapiapt_BR
dc.titleSynthesis and characterization of photosensitive gelatin-based hydrogels for photodynamic therapy in HeLa-CCL2 cell linept_BR
dc.typeArtigos de Periódicospt_BR

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