Replacement of the carbon catabolite regulator (cre1) and fed-batch cultivation as strategies to enhance cellulase production in Trichoderma harzianum

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dc.contributor.authorDelabona, Priscila da Silva
dc.contributor.authorLima, Deise Juliana
dc.contributor.authorCodima, Carla Aloia
dc.contributor.authorRamoni, Jonas
dc.contributor.authorGelain, Lucas
dc.contributor.authorMelo, Vandierly Sampaio de
dc.contributor.authorFarinas, Cristiane Sanchez
dc.contributor.authorPradella, José Geraldo da Cruz
dc.contributor.authorSeiboth, Bernhard
dc.date.accessioned2025-04-10T18:36:44Z
dc.date.available2025-04-10T18:36:44Z
dc.date.issued22021
dc.description.abstractThis work focused on mitigating carbon catabolic repression (CCR) and increasing cellulase production in Trichoderma harzianum based on the cre1 deletion. The CRE1 protein (encoded by cre1) has been described as a cellulase transcriptional repressor in various cellulotic fungi, but has not been investigated in T. harzianum. We constructed ∆cre1 T. harzianum by replacing the cre1 gene with the amdS gene from Aspergillus nidulans. Quantitative PCR analysis of some Cazymes genes showed that CRE1 acts positively on gh61, bgl1 and xyn2. The fed-batch strategy using hydrothermal sugarcane bagasse by the ∆cre1_Th15 produced a constant rate of FPase under glucose influence, suggesting that the knockout of the carbon catabolite regulator improved the glycoside hydrolases (FPase 1.96 ± 0.32 IU/mL; β-glucosidase 5.67 ± 0.28 IU/mL and xylanase 327. 26 ± 14.25 IU/mL), so that this strain can be used for biorefinery purposes.
dc.description.physical7 p.
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriCNPq (233875/2014-0)
dc.format.mimetypePDF
dc.identifier.affiliationNational Research Centre for Energy and Material
dc.identifier.affiliationInstitute of Chemical Engineering
dc.identifier.affiliationUniversidade Federal de Goiás
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationDELABONA, Priscila da Silva et al. Replacement of the carbon catabolite regulator (cre1) and fed-batch cultivation as strategies to enhance cellulase production in Trichoderma harzianum. Bioresource Technology Reports, v. 13, p. 1-7, 2021. Disponível em:
dc.identifier.doi10.1016/j.biteb.2021.100634
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/835
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderBioresource Technology Reports
dc.subject.keywordCarbon catabolic repression
dc.subject.keywordFed-batch
dc.subject.keywordTrichoderma harzianum
dc.subject.keywordGlycoside hydrolases
dc.titleReplacement of the carbon catabolite regulator (cre1) and fed-batch cultivation as strategies to enhance cellulase production in Trichoderma harzianum
dc.typeArtigos de Periódicos

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