Hydroxyapatite microspheres used as a drug delivery system for gliosarcoma strain 9l/Lacz treatment by photodynamic therapy protocols

Ambrósio, Jéssica Aparecida Ribeiro; Marmo, Vitor Luca Moura; Gonçalves, Érika Peterson; Pinto, Juliana Guerra; Ferreira-Strixino, Juliana; Raniero, Leandro José; Beltrame Junior, Milton; Simioni, Andreza Ribeiro

Hydroxyapatite microspheres used as a drug delivery system for gliosarcoma strain 9l/Lacz treatment by photodynamic therapy protocols

dc.contributor.author	Ambrósio, Jéssica Aparecida Ribeiro
dc.contributor.author	Marmo, Vitor Luca Moura
dc.contributor.author	Gonçalves, Érika Peterson
dc.contributor.author	Pinto, Juliana Guerra
dc.contributor.author	Ferreira-Strixino, Juliana
dc.contributor.author	Raniero, Leandro José
dc.contributor.author	Beltrame Junior, Milton
dc.contributor.author	Simioni, Andreza Ribeiro
dc.date.accessioned	2025-03-21T14:29:49Z
dc.date.available	2025-03-21T13:52:27Z
dc.date.available	2025-03-21T14:29:49Z
dc.date.issued2	2023-10-16
dc.description.abstract	Background: Hydroxyapatite (HAp) presents similarities with the human bone structure and presents properties such as biodegradability, biocompatibility, and osteoconductivity, which favors its use in prostheses implants and enables its use as a vehicle for the delivery of photosensitizers (PS) from systems of release (DDS) for photodynamic therapy applications Methods: In this work was to synthesized hydroxyapatite microspheres (meHAp), encapsulated with chloroaluminium phthalocyanine (ClAlPc), for DDS. meHAp was synthesized using vaterite as a template. The drug was encapsulated by mixing meHAp and a 50.0 mg.mL− 1 ClAlPc solution. Photochemical, photophysical, and photobiological studies characterized the system. Results: The images from the SEM analysis showed the spherical form of the particles. All spectroscopic results showed excellent photophysical parameters of the drug studied when served in the meHAp system. The incorporation efficiency was 57.8 %. The trypan blue exclusion test results showed a significant reduction (p < 0.05) in cell viability for the groups treated with PDT at all concentrations above 250 μg.mL− 1 . In 9 L/lacZ gliosarcoma cells, PDT mediated at concentrations from 250 to 62.5 µg.mL− 1 reduced cell viability by more than 98 %. In the cell internalization study, it was possible to observe the internalization of phthalocyanines at 37 ◦C, with the accumulation of PS in the cytoplasm and inside the nucleus in the two tested concentrations. Conclusions: From all the results presented throughout the article, the meHAp system shows promise for use as a modified release system (DSD) in photodynamic therapy.
dc.description.physical	9 p.
dc.description.sponsorship	Financiadora de Estudos e Projetos (Finep)
dc.description.sponsorship	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uri	FINEP n. 01.13.0275.00 CAPES) Code 001
dc.format.mimetype	pdf
dc.format.mimetype	jpeg
dc.identifier.affiliation	Universidade do Vale do Paraíba
dc.identifier.bibliographicCitation	AMBROSIO, J. A. R. et al. Hydroxyapatite microspheres used as a drug delivery system for gliosarcoma strain 9l/Lacz treatment by photodynamic therapy protocols. Photodiagnosis and Photodynamic Therapy, [S. l.], v. 44, p. 103830, dez. 2023. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1572100023005574..
dc.identifier.doi	10.1016/j.pdpdt.2023.103830
dc.identifier.uri	https://repositorio.univap.br/handle/123456789/738.2
dc.language.iso	en_US
dc.publisher	Elsevier
dc.rights.holder	Elsevier B.V.
dc.subject.keyword	Chloroaluminium phthalocyanine
dc.subject.keyword	Hydroxyapatite
dc.subject.keyword	Photodynamic therapy
dc.subject.keyword	Vaterite
dc.title	Hydroxyapatite microspheres used as a drug delivery system for gliosarcoma strain 9l/Lacz treatment by photodynamic therapy protocols
dc.type	Artigos de Periódicos