Vaterite submicron particles designed for photodynamic therapy in cells

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dc.contributor.authorSouza, Eliane de Fátima
dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorPinto, Bruna Cristina dos Santos
dc.contributor.authorBeltrame Junior, Milton
dc.contributor.authorSakane, Kumiko Koibuchi
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorGonçalves, Érica Peterson
dc.contributor.authorSimioni, Andreza Ribeiro
dc.date.accessioned2023-09-12T16:51:31Z
dc.date.available2023-09-12T16:51:31Z
dc.date.issued22020-07-06
dc.description.abstractBackground: Calcium carbonate (CaCO3) is one of the most abundant materials in the world. It has several different crystalline phases as present in the minerals: calcite, aragonite and vaterite, which are anhydrous crystalline polymorphs. Regarding the preparation of these microparticles, the most important aspect is the control of the polymorphism, particle size and material morphology. This study aimed to develop porous microparticles of calcium carbonate in the vaterite phase for the encapsulation of chloro-aluminum phthalocyanine (ClAlPc) as a photosensitizer (PS) for application in Photodynamic Therapy (TFD). Methods: In this study, spherical vaterite composed of microparticles are synthesized by precipitation route assisted by polycarboxylate superplasticizer (PSS). The calcium carbonate was prepared by reacting a mixed solution of Na2CO3 with a CaCl2 solution at an ambient temperature, 25 °C, in the presence of polycarboxylate superplasticizer as a stabilizer. The photosensitizer was incorporated by adsorption technique in the CaCO3 microparticles. The CaCO3 microparticles were studied by scanning electron microscopy, steady-state, and their biological activity was evaluated using in vitro cancer cell lines by trypan blue exclusion method. The intracellular localization of ClAlPc was examined by confocal microscopy. Results: The CaCO3 microparticles obtained are uniform and homogeneously sized, non-aggregated, and highly porous microparticles. The calcium carbonate microparticles show an average size of 3 μm average pore size of about 30–40 nm. The phthalocyanine derivative loaded-microparticles maintained their photophysical behavior after encapsulation. The captured carriers have provided dye localization inside cells. The in vitro experiments with ClAlPc-loaded CaCO3 microparticles showed that the system is not cytotoxic in darkness, but exhibits a substantial phototoxicity at 3 μmol.L−1 of photosensitizer concentration and 10 J.cm-2 of light. These conditions are sufficient to kill about 80 % of the cells. Conclusions: All the performed physical–chemical, photophysical, and photobiological measurements indicated that the phthalocyanine-loaded CaCO3 microparticles are a promising drug delivery system for photodynamic therapy and photoprocesses.pt_BR
dc.description.physical7 f.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.format.mimetypepdfpt_BR
dc.identifier.affiliationUnivappt_BR
dc.identifier.bibliographicCitationSOUZA, E. F.; AMBROSIO, J. A. R. ; PINTO, B. C. S. ; BELTRAME JUNIOR, M. ; SAKANE, K. K. ; PINTO, J. G. ; FERREIRA-STRIXINO, J. ; GONCALVES, E. P. ; Simioni, A. R. . Vaterite Submicron Particles Design for Photodynamic Therapy in Cells. Photodiagnosis and Photodynamic Therapy, v. 31, p. 101913-101919, 2020.pt_BR
dc.identifier.doi10.1016/j.pdpdt.2020.101913
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/315
dc.language.isopt_BRpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Restritopt_BR
dc.rights.holderSouza, Eliane de Fátimapt_BR
dc.rights.holderAmbrosio, Jéssica Aparecida Ribeiropt_BR
dc.rights.holderPinto, Bruna Cristina dos Santospt_BR
dc.rights.holderBeltrame Júnior, Miltonpt_BR
dc.rights.holderSakane, Kumiko Koibuchipt_BR
dc.rights.holderPinto, Juliana Guerrapt_BR
dc.rights.holderFerreira-Strixino, Julianapt_BR
dc.rights.holderGonçalves, Érica Petersonpt_BR
dc.rights.holderSimioni, Andreza Ribeiropt_BR
dc.subject.keywordPhotosensitizerpt_BR
dc.subject.keywordVaterite submicron particlespt_BR
dc.subject.keywordCalcium carbonatept_BR
dc.subject.keywordPhotodynamic therapypt_BR
dc.subject.keywordTerapia fotodinâmicapt_BR
dc.titleVaterite submicron particles designed for photodynamic therapy in cellspt_BR
dc.typeArtigos de Periódicospt_BR

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