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Item Exploring Antioxidant, Antimicrobial and Anti-Inflammatory Effects of Juglans regia and Pfaffia paniculata Extracts: implications for Intestinal Dysbiosis and Colorectal Cancer Risk Associated with Oral Pathogens(MDPI) Miranda, Diego Garcia; Ramos, Lucas de Paula; Attik, Nina; Silva, Nicole Van Der Heijde Fernandes; Camargo, Pyetra Claro; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Marcucci, Maria Cristina; Soares, Cristina Pacheco; Godoi, Bruno Henrique; Caires, Giovanna Arruda; Vigerelli, Hugo; Carrouel, FlorenceColorectal neoplasms rank as the third most prevalent cancer globally and stand as the second leading cause of cancer-related mortality. Its etiol- ogy is multifaceted, pointing to the role of microorganisms within the human microbiota in its development. Notably, the high prevalence of oral pathogens like Fusobacterium nucleatum and Parvimonas micra is implicated in inducing gut dysbiosis and stimulating the proliferation and metastasis of cancer cells. Therefore, this study aimed to evaluate in vitro the biological effects of extracts from Juglans regia and Pfaffia paniculata. Methods: Phytochemical analysis was carried out by HPLC, and the antioxidant effect was deter- mined by DPPH. Antimicrobial activity was investigated on F. nucleatum and P. micra planktonic and biofilms. Metabolic activity and genotoxicity were performed. Results: J. regia and P. paniculata expressed CE50 37.26 and 1367.57 mcg, respectively. The extracts exhibited a minimum bactericidal concentration of 1.73 and 0.48 mg/mL for J. regia and P. paniculata, respectively. Reduction superiorly 90% of P. micra biofilms. Metabolic activity was varied proportionally to the extract concentration, and no genotoxic effects were ob- served. Conclusions: The J. regia extract has great antioxidant activity and could be used as an alternative in combating pathogens associated with the onset of dysbiosis and tumor progression in colorectal neoplasms. Nevertheless, further studies are needed to validate their clinical applicability.Item Hyaluronic Acid-Bupivacaine Hydrogel System for Temporomandibular Joint disorders: Mechanical Lubrification and Local Anesthesia in a preclinical approach(Elsevier) Miranda, Diego Garcia; Ramos, Lucas de Paula; Araujo, Gabriela Ferraz de; Lopes, Nicole Fernanda dos Santos; Sani-Taiariol, Thalita; Baldan, Mauricio Ribeiro; Godoi, Bruno Henrique; Pacheco-Soares, Cristina; Gritsch, Kerstin; Borges, Alexandre Luiz Souto; Grosgogeat, BrigitteTemporomandibular disorders (TMD) are primarily characterized by pain and impaired mobility, affecting 5-12% of the adult population and significantly compromising quality of life. Current therapeutic management is multimodal but suffers from high failure rates, highlighting the need for improved interventions. We hypothesized the combination of hyaluronic acid (HA) with bupivacaine (BUP) in the search for a cytocompatibility hydrogel with improved physicochemical properties, suitable for intra-articular therapy of TMD, aiming at physical action, with the viscosity of HA and the prolonged analgesic effects of BUP in a single platform. FTIR analysis confirmed the presence of functional groups of both components. TGA revealed that the HA matrix significantly increased the thermal stability of BUP, delaying its decomposition. Rheological tests demonstrated predominant viscoelastic behavior (G' > G''), with adjustable stiffness depending on the concentration of BUP. Cytocompatibility assays on RAW 264.7 cells (OECD 129) showed that the HA-BUP formulation-maintained cell viability above 98%, in contrast to the use of the anesthetic (BUP) alone (53.6%), and did not exhibit genotoxicity (OECD 487). These findings conclude that the HA-BUP hydrogel has adequate biomechanical, thermal, and biocompatible properties in the laboratory setting. In vivo and clinical studies are needed to evaluate long-term efficacy and safety