3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
Item A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection(Frontiersin) Razzino, Claudia do Amaral; Sgobbi, Lívia Flório; Cancino-Bernardi, Juliana; Zapata, Angelica Maria Mazuera; Costa, Clara Cardoso; Zucolotto, Valtencir; Vieira, Lucia; Lobo, Anderson OliveiraAlzheimer’s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of β-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer’s disease is the decreased level of soluble β-amyloid peptide (1–42) (Aβ1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aβ1-42 levels drop below 192 pg mL-1 . In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aβ1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of −200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL-1 , with a detection limit of 7.4 pg mL-1 , exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer’s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.Item Correction: Barbosa et al. Production of rGO-Based Electrospinning Nanocomposites Incorporated in Recycled PET as an Alternative Dry Electrode. Polymers 2022, 14, 4288(MDPI) Barbosa, Michelle Chizzolini; Razzino, Claudia do Amaral; Stocco, Thiago Domingues; Santana, Moisés das Virgens; Ghosh, Anupama; Pereira, Luiz Fernando; Tierra-Criollo, Carlos Julio; Lobo, Anderson OliveiraItem Production of rGO-Based Electrospinning Nanocomposites Incorporated in Recycled PET as an Alternative Dry Electrode(MDPI) Barbosa, Michelle Chizzolini; Razzino, Claudia do Amaral; Stocco, Thiago Domingues; Santana, Moisés das Virgens; Ghosh, Anupama; Pereira, Luiz Fernando; Tierra-Criollo, Carlos Julio; Lobo, Anderson OliveiraIn this work, Coca-Cola® bottles were reused as a PET polymer (rPET) source to produce electrospun polymeric nanofibers. The nanofibers were electrospun from polymer solutions with different concentrations of reduced graphene oxide (rGO) incorporated for applications in somatosen-sory electrical stimulation. The rPET/rGO nanofiber mats were characterized by SEM, TEM, Raman, DSC, TGA, and DMA and the results showed that the incorporation of rGO in electrospun rPET fibers produced rPET/rGO composites. The rPET/rGO composites were then evaluated for possible appli- cation as dry electrodes. Moreover, with a preliminary test of numerous volunteers, the rPET/rGO dry electrode showed promising results. The rPET/rGO electrodes showed good performance and applicability to make dry electrodes, and these have applications as dry or wearable electrodes to produce electrochemical sensors.