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Item Analysis of the Protective Potential of the Amniotic Membrane in an In Vitro Experimental Model of Demyelination in Mouse Brain Organotypic Slices(ACS Publications) Guimarães, Melissa; Calheiro, Gabriela Antonia Tie; Sant'Anna, Luciana BarrosAmniotic membrane (AM) is a biological material recognized for its regenerative, anti-inflammatory, and immunomodulatory properties, constituting a promising approach for the treatment of neurodegenerative diseases, such as demyelinating diseases. Some neurodegenerative diseases, such as Multiple Sclerosis (MS), occur with demyelination, which is a process characterized by the loss of myelin, a structure responsible for the adequate conduction of nerve impulses, compromising neuronal functionality. In this context, this study aimed to investigate the efficacy of AM in protecting nervous tissue against the demyelinating effects of lysophosphatidylcholine (LPC, lysolecithin), using organotypic brain slices from C57BL/6 mice as an in vitro experimental model. Four experimental groups were established: C−H (healthy slices), C- DEM (slices demyelinated with LPC), C-AM (healthy slices with AM), and AM-LPC (slices protected by AM before LPC). The analyses included histological staining (Hematoxylin and Eosin, Luxol Fast Blue), metabolic test with 2,3,5-triphenyltetrazolium chloride (TTC), and Scanning Electron Microscopy (SEM). Results showed that AM preserved myelin and tissue architecture in the challenged slices, while the demyelination group presented microcavitations, structural disorganization, and loss of distinction between white and gray matter. The TTC assay revealed high metabolic activity in the slices protected by AM, in contrast with the low activity in the demyelinated group. SEM analysis reinforced the efficacy of AM, evidencing a preserved organization of the brain parenchyma in slices protected by AM. Thus, the results demonstrate that AM was effective in protecting nervous tissue against the demyelinating effects of lysophosphatidylcholine, preserving myelin, structural organization, and metabolic activity of brain slices, as evidenced by histological, metabolic, and ultrastructural analyses with SEM.Item Os benefícios do uso do enxerto da Membrana Amniótica Humana no âmbito de feridas cirúrgicas, ferimentos e lesões(Acervo +) Gonçalves, Nicolas Cardoso; Ribeiro, David Pinto; Gouvea, Hilda Cristina Rodrigues; Pedroso, Katia Zeny Assumpção; Silva, Nilson Thiago De Carvalho e; Sant'Anna, Luciana BarrosRealizar uma busca na literatura a respeito dos benefícios associados ao uso do enxerto da membrana amniótica humana (MAH), no âmbito ambulatorial e cirúrgico. Métodos: Trata-se de uma revisão integrativa cuja as bases de pesquisas foram: SciELO, PubMed, Biblioteca Virtual em Saúde e Capes. Foram utilizados estudos in vivo entre 2018-2023. Resultados: Após aplicação dos critérios de inclusão e exclusão, foram identificados 23 artigos. A membrana amniótica humana obteve resultados satisfatórios no tratamento de lesões cirúrgicas, queimaduras, úlceras, lesões na córnea, feridas agudas e crônicas, lesões em nervos, tendões, cartilagem e entre outras. Os estudos relataram que a MAH possui analgesia, ação anti-inflamatória e antimicrobiana, promove a epitelização e a cicatrização, reduz a fibrose, a substituição de curativos e o desconforto em realizar atividades e possui biocompatibilidade. Considerações finais: Dessa forma, esse estudo conseguiu investigar os diversos benefícios relativos ao transplante de MAH para o tratamento de lesões e ferimentos. O uso da MAH é uma possibilidade promissora no âmbito ambulatorial e cirúrgico, tendo em vista que possuem diversos benefícios para a cicatrização e epitelização de uma lesão.Item Histological analysis of Spinal Cord Injury treated with Amniotic Membrane(Universidade de São Paulo) Correia, Débora Campos Chaves; Lima, Leonardo Borges de; Sant'Anna, Luciana Barros; Lima, Mario Oliveira; Arisawa, Emilia Angela Lo SchiavoSpinal cord injury (SCI) is one of the most harmful syndromes that affects humans due to neuronal destruction and interruption of the nerve impulse transmission between axons. The conduction of motor, sensory, and autonomic responses below the level of the injury is seriously compromised, generating high treatment costs for the health system and a reduction in quality of life, stimulating research into new treatment protocols. This study aimed to evaluate the effectiveness of a biomaterial, the amniotic membrane (AM), to treat experimentally induced SCI. 15 adult rats were divided into three groups (n = 5): S (Sham), L (SCI without treatment), and AM (SCI treated with AM). Spinal cord injury was induced in the region T9-T10 by direct trauma, free-falling a weight (10 g, 2 mm flat edge) held on a mini guillotine, 25 mm above the exposed spinal cord. A fragment of AM, obtained from the human placenta after maternal consent, was applied to the injured area only in the AM group. After 28 days, specimens from the area of spinal cord injury were excised and subjected to routine histological procedures. Data from the semi-quantitative score, obtained from a scheme that assigned different scores to regions of the spinal cord, and from the quantitative analysis were subjected to parametric statistical analysis. Results showed that Group S presented medullary tissue without changes (score 0). In contrast, Group L presented numerous areas of cavitation in the dorsal and lateral regions of the white and gray matter (9.61 ± 6.60 p<0.001) with an intense inflammatory infiltrate. The AM group exhibited small areas of cavitation in the dorsal and lateral regions of the white matter and part of the dorsal columns in the gray matter (0.94 ± 1,03, p<0.001), with few inflammatory cells. The results suggest the effectiveness of AM in the treatment of induced SCI, characterized by a reduction in the evolution of inflammatory and degenerative processes in the central nervous tissue compared to the untreated group.