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Item Biomodulatory effect of low intensity laser (830 nm.) in neural model 9L/lacZ(CDRR Editor) Zabeu, Antonieta Marques Caldeira; Carvalho, Isabel Chaves Silva; Soares, Cristina Pacheco; Silva, Newton Soares daCurrently, research is advancing with low-intensity laser (LIL) in cells of the central nervous system, with the aim of evaluating the benefits of this therapy in neurological disorders such as Alzheimer's, stroke, ischemia, epilepsy, among others. The aim of this study was to verify the biomodulatory and biostimulatory effects of LIL in neural cell culture. Diode laser at wavelength λ = 830 nm, power 40 mW, in continuous mode, was applied on the 9L/lacZ cell line with energy densities of 0.5 to 3 J/cm . The analysis was performed 24 hours after irradiation, the results of cell viability showed a difference between the control and irradiated groups. As for the occurrence of apoptosis, no significant manifestation was observed between the control group compared to the irradiated group (P = 0.9956); there was a significant difference between apoptosis and death by necrosis between the control and treated groups (P<0.001). In the comet assay no statistically significant differences were observed. Regarding the objective of evaluating whether LIL promotes early activation of apoptosis or proliferation of 9L/lacZ cells at different energy densities of the infrared diode laser, we observed an increase in the number of neural cells, highlighting the action of biomodulation. Furthermore, LIL did not promote the activation of programmed cell death - apoptosis and did not show any indication of DNA damage by the comet assay. The results of this study are indicative that the laser in the near infrared has a positive interaction with neuronal cells.Item In vitro evaluation of red and near infrared LED photobiomodulation on L929 fibroblasts metabolic activity and morphology(Springer) Segismondi, Larissa Cavallieri; Soares, Luís Eduardo Silva; Soares, Cristina PachecoPurpose Fibroblasts, the main cells of connective tissue, are highly susceptible to oxidative stress caused by reactive oxygen species (ROS). Photobiomodulation (PBM) has emerged as a promising therapy capable of modulating biological tissues, enhancing cellular metabolic activity, and promoting the proliferation and survival of fibroblasts. In this study, we aimed to investigate the potential in vitro photoprotective effect of cellular photomodulation using 660 and 850 nm LEDs in L929 fibroblast cells treated with hydrogen peroxide as a model of oxidative stress. Methods Changes in cell viability were observed using the Alamar Blue colorimetric assay, and cell morphology was assessed by inverted microscopy. Mitochondria and nuclei were also labeled in living cells using fluorescence with TMRM and Hoechst, in addition to ROS detection with CellRox Green. Results Our results indicate that pretreatment with LED exerts a cytoprotective effect against oxidative stress, promoting an increase in mitochondrial activity, mitochondrial membrane potential, and a reduction in intracellular reactive oxygen spe- cies (ROS) generation while inducing improvements in the morphological characteristics of cells. Conclusion The findings from the present study indicate that Photobiomodulation (PBM) with LED contributes to maintain- ing cellular homeostasis and can help prevent and mitigate damage resulting from oxidative stress in fibroblasts.