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Item Photobiomodulation applied in post-surgical female-to-male surgery: case report(Erciyes Üniversitesi) Arisawa, Emilia Angela Lo Schiavo; Martins, Rodrigo Álvaro Lopes; Martins, Patrícia Sardinha Leonardo Lopes; Meneguzzo, Daiane Thais; Arisawa, Ana Amélia Lo Schiavo; Gallagher, SidhbhPhotobiomodulation (PBM) is an emerging medical technology with therapeutic effects that reduce or inhibit inflammatory mediators and cytokines, decreasing pain and local edema. However, the number of papers reporting the application of PBM in the post-surgical period is still scarce. This case report aims to evaluate the action of PBM applied after bilateral mastectomy with free nipple grafts regarding collateral effects such as pain, edema and hematoma. Patients underwent breast surgery with free nipples grafts. PBM sessions were applied 5, 24, 48 and 72 hours after surgery using a diode laser Platinum 4 equipment with four wavelengths (650, 810, 915 and 980 nm). Total energy applied was 1080 Joules distributed in six minutes of laser application over lymph node areas and the suture region. Both patients (A and B) related pain relief after PBM sessions (5, 24, 48 and 72 hours) after surgery. Also nipple sensitivity and overflow of reddish-color were reduced after 72h. The application of PBM reduced pain intensity and nipples hypersensitivity, suggesting reduction of inflammatory process. The benefits reported in this study suggest the indication of this complementary therapy to cosmetic procedures for transgender people.Item Infrared thermography as valuable tool for gynoid lipodystrophy (cellulite) diagnosis(Springer-Verlag London Ltd.) Lopes‐Martins, Rodrigo Álvaro Brandão; Barbaroto, Douglas Peretta; Barbosa, Elaine da Silva; Leonardo, Patrícia Sardinha; Ruiz-Silva, Carlos; Arisawa, Emília Ângela Lo SchiavoCellulite is a morphological alteration of the tegument tissue, directly interfering in self-esteem with etiology and pathophysiology far from being a consensus. Although the visual diagnosis of cellulitis is well known, it does not represent the real pathological condition of the subcutaneous tissue. The aim of the study was to investigate the hypothesis that the more heterogeneous tissue pattern analyzed by infrared thermography, the more severe is the cellulite grade. Forty female participants were selected and 60 thighs were analyzed by clinical anamnesis and infrared thermography. Classical visual analysis was correlated to the tissue heterogeneity measured by thermography. R Spearman’s correlation between visual evaluation and thermography was 0.92. Phototype presented a negative significant correlation of 0.67 with classical visual analysis. In the present study, we presented a simple method based on infrared thermography that can be adopted in any esthetics office with a correlation of 0.92 with the visual classic evaluation, but, besides, may be very helpful to the clinician to decide which treatment will be adopted, i.e., an aggressive and inflammatory approach such as the radiofrequency of shockwave therapy or an anti-inflammatory approach such as photobiomodulation, depending on the inflammatory status of cellulite.Item Cyto-genotoxicity and immunomodulation in fibroblasts exposed to calcium-aluminate cement with different radiopacifiers(Fundação Odontológica de Ribeirão Preto) Martorano, Antônio Secco; Messias, Nadyne Saab; Bighetti-Trevisan, Rayana Longo; Teixeira, Lucas Novaes; Oliveira, Ivone Regina de; Raucci-Neto, Walter; Oliveira, Paulo Tambasco de; Castro-Raucci, Larissa Moreira Spinola deCalcium aluminate cement (CAC) has been proposed as an alternative to mineral trioxide aggregate (MTA), but its biological behavior remains underexplored. This study evaluated the cytotoxicity, genotoxicity, and proinflammatory gene expression of fibroblasts exposed to CAC formulations containing zinc oxide or bismuth oxide with different proportions of calcium chloride, in comparison with MTA. Fibroblasts were cultured with CACz (25% ZnO + 2.8% CaCl₂), CACb (25% Bi₂O₃ + 2.8% CaCl₂), CACb+ (25% Bi₂O₃ + 10% CaCl₂), or MTA; non-exposed cells served as controls. Cytotoxicity was assessed by fluorescence microscopy and MTT viability assays at 24 h and 72 h, while genotoxicity was evaluated by the comet assay at 24 h. Gene expression of COL-1, TNF-α, IL-1β, IL-6, and MMP-9 was examined by RT-qPCR at 72 h. Data were analyzed using the Kruskal-Wallis test and the Student-Newman-Keuls post hoc test (α = 0.05). Cell viability did not differ among groups at 24 h, but was higher for CACb+ at 72 h (p < 0.05). DNA damage levels were similar across groups (p > 0.05). For gene expression, no significant differences were observed for COL-1, IL-1β, or TNF-α. All cements upregulated IL-6 compared with control (p < 0.05), with the highest levels in MTA and the lowest in CACb+. CACb+ increased MMP-9 expression (p < 0.05), while CACz showed the lowest levels. Within the limits of this study, CAC formulations with additives were not genotoxic or cytotoxic, and their biological behavior was composition-dependent, with CACb+ demonstrating the most favorable profile by enhancing fibroblast viability and extracellular matrix remodeling potential.