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Item Combined morphological-compositional analysis of the interaction of collagen and chitosan based-materials with demineralized bovine dentin(Brazilian Journals Publicações de Periódicos) Nahórny, Sidnei; Oliveira, Ivone Regina de; Soares, Luís Eduardo SilvaTreatments to promote the biomineralization of dentin are the focus of much research with different materials and processes. Biomineralization is a process mediated by an organic matrix in which organic macromolecules act as models for the nucleation and growth of mineral crystals to form hierarchically ordered hybrid materials, such as bones or teeth. This study describes the application of novel materials based on collagen and chitosan aimed to protect the dentin from erosive conditions. The dentin morphology of the bovine teeth treated with these materials was visualized using scanning electron microscopy (SEM). In additions, it was possible to confirm the data by analyzing the distribution of inorganic content of dentin by micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF). The association of analytical techniques demonstrated greater surface coverage for the chitosan-fluoride followed by the hydrolyzed collagen. Both materials are promising for the application of dentin coverage in dentistry.Item Influence of fluoride characteristics on tooth surface protection in an erosive condition: a multifaceted characterization approach(Wiley) Silva, Marcelo Henrique Pereira Marques da; Laurindo, Vinicius Silva; Soares, Luís Eduardo SilvaAbstract Objective: To evaluate the effect of fluoride consistency and composition to protect enamel and dentin against the dental erosion. Materials and Methods: Bovine enamel and dentin specimens were treated with arti- ficial saliva, neutral fluoride gel (NFG), acidulated phosphate fluoride gel (AFG), neu- tral fluoride foam (NFF), and acidulated phosphate fluoride foam. The samples were subjected to cycling. Micro energy-dispersive X-ray fluorescence spectrometry, sur- face roughness (Ra), contact angle (CA), and scanning electron microscopy (SEM) were performed. Composition, CA and Ra data were analyzed by ANOVA and multi- ple comparison test (p < 0.05). Results: The dentin protected had a significantly higher mineral content than in the control. Eroded unprotected enamel had higher Ra values than normal surfaces. Fluo- ride treatments increased the Ra in dentin samples. AFG increased the CA in enamel. Fluoride foams increased CA in dentin with reduced mineral loss. SEM analysis found a deposited layer on enamel treated with AFG and remnants of deposits on dentin treated with NFG and NFF. Conclusion: Regardless of the form of application, fluoride provided protection against erosion, however with different levels. Clinical Significance: Applying the adequate fluoride form is relevant since the for- mulations have different effects on both enamel and dentin.