Programa de Pós-Graduação em Engenharia Biomédica

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Navegando Programa de Pós-Graduação em Engenharia Biomédica por Assunto "Alzheimer's Disease"

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    Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
    (CDRR Editors) Rossato, Rafaella Carvalho; Granato, Alessandro Eustaquio Campos; Moraes, Carlos Dailton Guedes de Oliveira; Salles, Geisa Nogueira; Soares, Cristina Pacheco
    Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.
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    Photobiomodulation by LED 660 nm and Taurine against H2O2 oxidative stress in SH-SY5Y cells
    (Springer Nature Link) Rossato, Rafaella Carvalho; Salles, Geisa Rodrigues; Albuquerque, Amanda Lira; Porcionatto, Marimélia Aparecida; Granato, Alessandro Eustáquio Campos; Ulrich, Henning; Santos, Mariela Inês Batista dos; Soares, Cristina Pacheco
    Alzheimer's Disease (AD) is a progressive uncurable neurodegenerative pathology affecting millions worldwide. Photo- biomodulation and Taurine are promising alternatives for preventing and reducing the rapid progression of neurodegenera- tion, stimulating the reconstructing of neural tissue structures, especially improving mitochondrial activity, which is highly impaired in AD. In this study, the mitochondrial effects of Taurine combined with light emitting diode (LED) irradiation were evaluated on human neuroblastoma cells (SH-SY5Y), under oxidative stress condition by hydrogen peroxide (H2O2) exposure, a considerable modulator in AD. We evaluated LED irradiation at the wavelength of 660 nm and Taurine under different concentrations before and together with exposing SH-SY5Y cells to different concentrations of H2O2, assessing mitochondrial activity by the MTT colorimetric test and labeling live cells mitochondria by the fluorescent probe MitoTracker. Cell viability was also evaluated by the trypan blue exclusion assay, and cellular morphological structures were imaged by scanning electron microscopy (SEM). Neuroprotective effects were achieved by both LED irradiation and LED irradia- tion + Taurine when cells were exposed to them before H2O2-induced stress. Comparing both agents, LED irradiation at 660 nm is sufficient to improve mitochondrial activity, however, healthy mitochondrial morphology was only observed when cells were treated with Taurine together with LED irradiation, representing affordable candidates that act in synergy against oxidative stress, one of the main contributors to neurodegeneration.