Antitumor activity of membranes associated with Acmella oleracea extract

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dc.contributor.authorSilva, Carlos Augusto Priante da
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorMenegon, Renato Farina
dc.contributor.authorSilva, Newton Soares da
dc.contributor.authorSoares, Cristina Pacheco
dc.date.accessioned2026-01-09T12:58:14Z
dc.date.available2026-01-09T12:58:14Z
dc.date.issued22024
dc.description.abstractEpithelial cancers, such as epidermoid cancer and some adenocarcinomas, affect surface areas that are generally more accessible to various treatments. However, this group of tumor cells has an aggressive behavior, leading to a high annual mortality rate. The development of a biomaterial that is non-invasive, can kill tumor cells, and prevent opportunistic infections is the basis for the treatment for this type of cancer. Therefore, the objective of this study was to develop a biomaterial from chitosan and A. oleracea extracts that exhibits cytotoxic action against the HEp-2 tumor cell line. Dried crude 90% ethanol extracts were obtained through ultrasound-assisted maceration, followed by liquid-liquid extraction to yield the butanol fraction. From these extracts, chitosan membranes were developed and evaluated for their antitumor activity against HEp-2 using viability tests with crystal violet and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, in addition to a wound healing test. The cytotoxic assays indicated a significant reduction in cell density and mitochondrial activity, especially at the concentration of 1000 mg/mL of crude extract. The butanol fraction had minimal effects on mitochondrial activity. The wound healing test demonstrated that the biomaterial and extract prevented closure of the wound created in the cell monolayer within 48 h of incubation and caused changes in cell morphology. In view of this, we concluded that a chitosan membrane associated with a 90% ethanol extract of Acmella oleracea exhibited cytotoxic activity is a potential alternative treatment for superficial cancers.
dc.description.physical8 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriCAPES: Finance Code 001
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual de São Paulo Júlio de Mesquita Filho
dc.identifier.bibliographicCitationSILVA, C. A. P. et al. Antitumor activity of membranes associated with Acmella oleracea extract. Brazilian Journal of Medical and Biological Research, v. 57, p. 1-8, 2024. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2024000100687&tlng=en.
dc.identifier.doi10.1590/1414-431x2024e14129
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1071
dc.language.isoen_US
dc.publisherAssociação Brasileira de Divulgação Científica
dc.rights.holderBrazilian Journal of Medical and Biological Research
dc.subject.keywordBiomaterial
dc.subject.keywordChitosan
dc.subject.keywordHEp-2 culture
dc.subject.keywordCancer
dc.titleAntitumor activity of membranes associated with Acmella oleracea extract
dc.typeArtigos de Periódicos

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