Produção acadêmica-PPGEB

URI Permanente para esta coleção

https://repositorio.univap.br/handle/123456789/83

Navegar

Agora exibindo 1 - 20 de 55

Combined morphological-compositional analysis of the interaction of collagen and chitosan based-materials with demineralized bovine dentin
(Brazilian Journals Publicações de Periódicos) Nahórny, Sidnei; Oliveira, Ivone Regina de; Soares, Luís Eduardo Silva
Treatments to promote the biomineralization of dentin are the focus of much research with different materials and processes. Biomineralization is a process mediated by an organic matrix in which organic macromolecules act as models for the nucleation and growth of mineral crystals to form hierarchically ordered hybrid materials, such as bones or teeth. This study describes the application of novel materials based on collagen and chitosan aimed to protect the dentin from erosive conditions. The dentin morphology of the bovine teeth treated with these materials was visualized using scanning electron microscopy (SEM). In additions, it was possible to confirm the data by analyzing the distribution of inorganic content of dentin by micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF). The association of analytical techniques demonstrated greater surface coverage for the chitosan-fluoride followed by the hydrolyzed collagen. Both materials are promising for the application of dentin coverage in dentistry.
Calcium Aluminate Cement Blends Containing Bioactive Glass and Strontium for Biomaterial Applications
(SciELO) Barbosa, Ana Margarida; Santos, Kennedy Wallace dos; Gonçalves, Irene S.; Leite, Priscila Maria Sarmeiro Corrêa Marciano; Martorano, Antonio Secco; Grisote, Gabriela; Raucci, Larissa Moreira Spinola de Castro; Oliveira, Paulo Tambasco de; Raniero, Leandro José; Oliveira, Ivone Regina de
In this work blends based on calcium aluminate cement (CAC) containing bioactive glass (BG) (5, 7.5 and 10 wt%) and strontium oxide (1 wt%) were produced aiming improve their bioactivity and the capacity to stimulate the bone regeneration. In the first part, the blends containing only BG were characterized as theoretical density, microhardness, uniaxial cold crush strength after SBF and apparent porosity and pore size distribution before and after SBF treatment. In the second part, bioactivity and cell culture tests were also conducted in the blends containing BG and strontium oxide. The addition of 7.5 wt% of BG in homogeneous calcium aluminate cement (CH) improved its mechanical properties as microhardness and uniaxial cold crushing strength. The blends were more bioactive due to the presence of a highly soluble amorphous phase as confirmed by means of SEM/EDX mainly for 7.5 wt% BG without and with Sr from 1 day in SBF. FTIR analyses indicated the formation the apatite-like phase by means of increase of intensity of the PO43- peaks after SBF treatment. All blends allowed the development of the osteoblastic phenotype and the formation of mineralized matrix increased due to the inclusion of BG and Sr promoting the osteogenesis process.
Amniotic membrane applied to burns healing: Pre-clinical study
(CDRR Editors) Amorim, Fernanda Cláudia Miranda; Arisawa, Emilia Ângela Loschiavo; Sant’Anna, Luciana Barros; Fonseca, Khetyma Moreira; Costa, Davidson Ribeiro; Rodrigues, Ana Beatriz Mendes; Carvalho, Jancineide Oliveira de
This preclinical study aimed to evaluate the tissue repair process of burns treated with human amniotic membrane (hAM) patches in rats. Twenty-four rats were subjected to superficial burns of partial thickness, and randomly allocated into two groups: Control and Treated Group, subdivided into two experimental periods of 7th and 14th days. The lesions were evaluated by digitalized images (macroscopy) and by the analysis of histological sections stained in H&E to quantify the number of inflammatory cells and fibroblasts present in the different experimental times (histomorphometry). The histomorphometric analyses were performed blindly. Statistical analysis employed Kolmogorov-Smirnov and Mann Whitney tests, with 95% confidence interval at 5% significance level (p <0.05). Macroscopically, the lesions of Treated group presented a crust formation before Control Group, and there were no signs of infection in both groups. Microscopically, the qualitative analysis showed a faster evolution in the healing process of the Treated groups compared to the Control, with reduction of the inflammatory infiltrate, intense fibroblasts proliferation and better organization of the collagen fibers. The quantitative analysis showed statistically significant results regarding the reduction of inflammatory cells (p<0.0001) at 7th and 14 th day and increased proliferation of fibroblasts at 14th day (p<0.0001) in lesions treated with hAM compared to Control group. The results of this preclinical study demonstrated that the application of hAM patches reduces the inflammatory process and accelerates the onset of the proliferative phase in burn injuries.
Development of a smartphone device for reading aimed at low-vision people
(Revista Univap) Silva, Bruno Procópio da; Mendes, Alessandro Correa; Lima, Fernanda Púpio Silva; Pinto, Ana Paula; Souza, Gabriela Aparecida da Silveira; Lopes-Martins, Rodrigo Alvaro Brandão; Lima, Mário Oliveira
Many people with total or partial visual impairment can use assistive technology (AT) to facilitate daily living activities. Smartphones and, especially, their applications, can be a tool of easy access and applicability as AT. The aim of this study was to develop an application for use on a smartphone or tablet to improve the visual ability of people with low visual acuity. The software, called Oftcam, was developed using the ANDROID operating system, written in Java Android under the Java version JDK 1.7, supporting the minimum version of Android 2.2. Its operating mechanism includes capturing and adjusting the image of interest to the users according to their needs: expansion, change of background and decentralization of the image of interest. The development of this free, easy-to-handle application will provide the possibility of integration between the user and the auxiliary professional, being, in practice, a mobile resource of health. Considering that most people have an increasing access to phones and tablets, we believe the use of this application is a good alternative to integrate the need and practicality in the daily lives of the visually impaired people.
Non-thermal plasma applied to treating diabetic foot
(CDRR Editors) Arisawa, Emilia Angela Lo Schiavo; Cardoso, Juliana Cunha; Vieira, Lucia; Oliveira, Rauirys Alencarde de
Objective: Evaluate the effects of non-thermal plasma therapy (NTP) for the treatment of diabetic foot injuries in two patients, considering the evolution of the steps of the healing process, pain sensitivity, and quality of life. Methodology: Lesions in the lower limbs of two patients with Diabetes mellitus (DM) with similar anatomical location and dimensions were treated, one patient with controlled DM and the other classified as uncontrolled DM. The application of NTP was performed for 10 minutes, 3 times a week in direct contact with the lesions. The same protocol was applied in both clinical cases to allow a reliable evaluation of the healing process. Results and Discussion: The lesions presented progressive regression until their complete regeneration (100%), without the development of infections during treatment. Conclusion: The results obtained in the two clinical cases allow us to attest that the NTP application in the treatment of diabetic foot injuries resulting from DM constituted an important therapeutic tool for the evolution of the healing process of these injuries, with a significant reduction in the lesion area in a short period of time. The definition of the ideal treatment protocol depends on the expansion of the number of studies and the increase of the studied population.
PCR analysis of the effect of photodynamic therapy on breast tumors
(CDRR Editors) Ferreira, Isabelle; Silva, Glenda Nicioli da; Ferreira-Strixino, Juliana; Grecco, Clovis; Bagnato, Vanderlei Salvador; Salvadori, Daisy Maria Favero; Pinto, Juliana Guerra; Rocha, Noeme Sousa
Photodynamic therapy (PDT) is a promising therapeutic modality for treating cancer, including breast tumors. The oxidative damage caused by PDT culminates in cell death, induction of immune response, and the resulting destruction of the tumor. This study aimed to evaluate the gene expression profiling of genes BCL-2, BAX, and HER-2 and their proteins after PDT, associating it with the necrosis caused by this therapy under different fluences. Twenty-eight female rats received a single dose of 7,12-dimethylbenz (a) anthracene (DMBA - 80mg/kg), by gavage, for breast tumor induction. After the tumors grew, the animals were divided into four groups: G1 - control group – untreated breast tumor – and G2, G3, and G4 groups treated with PDT using Photogem@ as photosensitizer and interstitial irradiation, with fluences of 50J/cm, 100J/cm, and 150J/cm, respectively. Samples of tumors were harvested for histological examination by RT-qPCR. The RT-qPCR showed that the gene expression profiling of BCL-2, BAX, and HER-2 was not altered after PDT. Hemorrhagic necrosis and qualitatively greater vascular and cellular damage were observed and correlated positively with the fluence. PDT does not seem to induce the modulation of genes related to apoptosis. The results indicate that the type of cell death stimulated by PDT in breast tumor is necrosis.
Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
(MDPI) Garcia, Maíra Terra; Ward, Rafael Araújo da Costa; Gonçalves, Nathália Maria Ferreira; Pedroso, Lara Luise Castro; Silva Neto, José Vieira da; Ferreira-Strixino, Juliana; Junqueira, Juliana Campos
Photodynamic therapy (PDT) mediated by Fotoenticine® (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium Streptococcus mutans. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of mutans streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.
PCR-RFLP and FTIR-based detection of high-risk human papilloma virus for cervical cancer screening and prevention
(Elsevier) Melo, Igor Martins Alves; Viana, Magda Rogéria Pereira; Pupin, Breno; Bhattacharjee, Tanmoy Tapobrata; Canevari, Renata de Azevedo
Background: Approximately 70% of cervical carcinoma cases show the presence of high-risk Human Papilloma Virus (HPV), especially HPV-16 and HPV-18, and can be used to stratify high risk patients from low risk and healthy. Currently, molecular biology techniques such as polymerase chain reaction (PCR) are used to identify the presence of virus in patient samples. While the methodology is highly sensitive, it is labor intensive and timeconsuming. Alternative techniques, such as vibrational spectroscopy, has been suggested as a possible rapid alternative. Therefore, in this study, we evaluate the efficiency of cervical fluid Fourier Transform Infrared spectroscopy (FTIR) in patient risk stratification informed by PCR. Methods: Cervical fluid samples (n = 91) were obtained from patients who have undergone routine Papanicolaou (Pap) test. Viral genome was identified and classified as high/low-risk by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). FTIR spectra were acquired from samples identified by PCR-RFLP as No-HPV (n = 10), high-risk HPV (n = 7), and low-risk HPV (n = 7). Results: Of the 91 samples, was detected the viral genome by PCR in 36 samples. Of these 36 samples, nine samples were identified to contain high-risk HPV (HR-HPV) and nine samples were found to have low-risk HPV (LR-HPV). The FTIR spectra acquired from No-HPV, LR-HPV, and HR-HPV showed differences in 1069, 1437, 1555, 1647, 2840, 2919, and 3287 cm-1 bands. Principal Component Analysis (PCA) showed distinct clusters for No-HPV and HR-HPV and No-HPV and LR-HPV, but there was significant overlap in the clusters of HR-HPV and LR-HPV. PCA-Linear Discriminant Analysis (PC-LDA) after Leave One Out Cross Validation (LOOCV) classified No-HPV from HR-HPV and No-HPV from LR-HPV with 100% efficiency in the 1400-1800 cm-1 spectral range. LOOCV classifications for LR-HPV and HR-HPV from each other were 71 and 75%, respectively, in the 2800-3400 cm-1 spectral range. Conclusions: The results highlight the high sensitivity of PCR-RFLP in HPV identification and show that FTIR can classify samples identified as healthy, low, and high-risk samples by PCR-RFLP. General significance: We show the possibility of using FTIR for initial cervical cancer risk stratification followed by detailed PCR-RFLP investigations for suspect cases.
Infrared thermography as valuable tool for gynoid lipodystrophy (cellulite) diagnosis
(Springer-Verlag London Ltd.) Lopes‐Martins, Rodrigo Álvaro Brandão; Barbaroto, Douglas Peretta; Barbosa, Elaine da Silva; Leonardo, Patrícia Sardinha; Ruiz-Silva, Carlos; Arisawa, Emília Ângela Lo Schiavo
Cellulite is a morphological alteration of the tegument tissue, directly interfering in self-esteem with etiology and pathophysiology far from being a consensus. Although the visual diagnosis of cellulitis is well known, it does not represent the real pathological condition of the subcutaneous tissue. The aim of the study was to investigate the hypothesis that the more heterogeneous tissue pattern analyzed by infrared thermography, the more severe is the cellulite grade. Forty female participants were selected and 60 thighs were analyzed by clinical anamnesis and infrared thermography. Classical visual analysis was correlated to the tissue heterogeneity measured by thermography. R Spearman’s correlation between visual evaluation and thermography was 0.92. Phototype presented a negative significant correlation of 0.67 with classical visual analysis. In the present study, we presented a simple method based on infrared thermography that can be adopted in any esthetics office with a correlation of 0.92 with the visual classic evaluation, but, besides, may be very helpful to the clinician to decide which treatment will be adopted, i.e., an aggressive and inflammatory approach such as the radiofrequency of shockwave therapy or an anti-inflammatory approach such as photobiomodulation, depending on the inflammatory status of cellulite.
Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases
(Royal Society of Chemistry) Cairo, João Paulo L. Franco; Mandelli, Fernanda; Tramontina, Robson; Cannella, David; Paradisi, Alessandro; Ciano, Luisa; Ferreira, Marcel R.; Liberato, Marcelo V.; Brenelli, Lívia B.; Gonçalves, Thiago A.; Rodrigues, Gisele N.; Alvarez, Thabata M.; Mofatto, Luciana S.; Carazzolle, Marcelo F.; Pradella, Jose Geraldo da Cruz; Leme, Adriana F. Paes; Leonardo, Ana M. Costa; Neto, Mário Oliveira; Damasio, André; Davies, Gideon J.; Felby, Claus; Walton, Paul H.; Squina, Fabio M.
Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown enzymatic activities in their digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays a previously unknown role in plant biomass degradation. We show that CgSOD-1 transcripts and peptides are up-regulated in response to an increased level of lignocellulose recalcitrance and that CgSOD-1 localizes in the lumen of the fore- and midguts of C. gestroi together with termite main cellulase, CgEG-1-GH9. CgSOD-1 boosts the saccharification of polysaccharides by CgEG-1-GH9. We show that the boosting effect of CgSOD-1 involves an oxidative mechanism of action in which CgSOD-1 generates reactive oxygen species that subsequently cleave the polysaccharide. SOD-type enzymes constitute a new addition to the growing family of oxidases, ones which are up-regulated when exposed to recalcitrant polysaccharides, and that are used by Nature for biomass degradation.
Local envenomation caused by a bioactive peptide fraction of Bothrops jararaca snake venom induces leukocyte influx in the lung and changes in pulmonary mechanics
(Elsevier) Silva, Carlos Alberto; Querobino, Samyr Machado; Silva, Cesar Augusto Melo; Costa, Maricilia Silva; Oliveira, Luis Vicente Franco; Zamuner, Stella Regina
The crude venom of the Bothrops jararaca snake (Bj-CV) is a complex mixture of biologically active proteins that includes a variety of peptides in the low molecular weight fraction (Bj-PF). We investigated how an intramuscular injection of Bj-CV (1.2 mg kg−1) and Bj-PF (0.24 mg kg−1) influenced lung mechanics and lung and muscle inflammation in male Swiss mice 15 min, 1, 6, and 24 h after inoculation. Pressure dissipation against lung resistive components (ΔP1) rose significantly from 1 to 24 h after Bj-CV and 6–24 h after Bj-PF inoculation. Both Bj-CV and Bj-PF increased the total pressure variation of the lung (ΔPtot) 24 h after injection. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Furthermore, intramuscular inoculation of Bj-CV and Bj-PF resulted in an increase in muscle and pulmonary inflammation, as evidenced by an increase in leukocyte influx when compared to the control group. Finally, both Bj-CV and Bj-PF cause acute lung injury, as shown by pulmonary inflammation and decreased lung mechanics. Furthermore, the fact that Bj-PF produces mechanical alterations in the lungs and muscular inflammation implies that non-enzymatic compounds can cause inflammation.
Estimation of the DNA damage in Girardia tigrina (Girard, 1850) after exposure to radiation under extreme conditions
(Brazilian Journals Publicações de Periódicos) Benitz, Tabatha; Oliveira, Matheus Salgado de; Lopes, Karla Andressa Ruiz; Soares, Cristina Pacheco; Morais, Flavia Villaça; Velho, Nádia Maria Rodrigues de Campos
The use of planarians as experimental models has been increased worldwide especially due to their high capacity of tissue regeneration and ease of laboratory maintenance. The current study aims to assess the genetic damage of Girardia tigrina after exposure of stressors in conditions such as: critical temperature, critical pH, hypergravity (4.4G) and low power laser (685nm). 18 specimens of G. tigrina were used, the extreme conditions mentioned were experimental group with 3 specimens each, and 3 for the control group, all specimens were submitted to the Comet Assay after exposure of stressors, and control group with no extreme condition exposure. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation, caused genetic damages to the G. tigrina, for hypergravity and low-power laser stressors the damage was higher in the pharyngeal and post-pharyngeal regions of the animal with a greatest area of comet tail dragging for the post-pharyngeal region. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation caused genetic damages in planarians Brazilian Journal of Development ISSN: 2525-8761 12160 Brazilian Journal of Development, Curitiba, v.8, n.2, p.12159-12177 feb. 2022. G. tigrina, especially in samples from the pharyngeal and post-pharyngeal regions of the flatworms body assessed by the Comet Assay.
Photodynamic effect of protoporphyrin IX in gliosarcoma 9l/lacZ cell line
(Elsevier B.V.) Fontana, Letícia Corrêa; Pinto, Juliana Guerra; Vitorio, Gabrielle dos Santos; Ferreira, Isabelle; Pacheco-Soares, Cristina; Mamone, Leandro; Ferreira‐Strixino, Juliana
Photodynamic Therapy (PDT) is an oncologic treatment, producing reactive oxygen species (ROS) to induce the death of cancer cells. This study aimed to evaluate the action of PDT on gliosarcoma cells, using protoporphyrin IX as PS by incubation with the precursor aminolevulinic acid (ALA). An LED device was used with a light dose of 10 J/cm². The success of the therapy proved to be dependent on the concentration of ALA, and an incubation time of 4 h required for an effective response. Cell death was prevalent due to necrosis when assessed 18 h post-PDT. ALA proved to be an option to PDT in cells of the 9 L/lacZ, with the protocol tested.
(MeOPhSe)2, a synthetic organic selenium compound, inhibits virulence factors of Candida krusei: Adherence to cervical epithelial cells and biofilm formation
(Elsevier GmbH.) Siqueira, Victor Mendes de; Silva, Bruna Graziele Marques da; Passos, Juliene Cristina da Silva; Pinto, Ana Paula; Rocha, João Batista Teixeira da; Silva, Carlos Alberto; Costa, Maricília Silva
Background: Systemic candidiasis is produced by Candida albicans or non-albicans Candida species, opportunistic fungi that produce both superficial and invasive infections. Despite the availability of a wide range of antifungal agents for the treatment of candidiasis, failure of therapy is observed frequently, which opens new avenues in the field of alternative therapeutic strategies. Methods: The effects of p,p′ -methoxyl-diphenyl diselenide [(MeOPhSe)2], a synthetic organic selenium (organochalcogen) compound, were investigated on virulence factors of C. krusei and compared with its antifungal effects on the virulence factors related to adhesion to cervical epithelial cell surfaces with C. albicans. Results: (MeOPhSe)2, a compound non-toxic in epithelial (HeLa) and fibroblastic (Vero) cells, inhibited the growth in a dose-dependent manner and changed the kinetics parameters of C. krusei and, most importantly, extending the duration of lag phase of growth, inhibiting biofilm formation, and changing the structure of biofilm. Also, (MeOPhSe)2 reduced C. albicans and C. krusei adherence to cervical epithelial cells, an important factor for the early stage of the Candida-host interaction. The reduction was 37.24 ± 2.7 % in C. krusei (p = 0.00153) and 32.84 ± 3.2 % in C. albicans (p = 0.0072) at 20 μM (MeOPhSe)2, and the effect is in a concentration-dependent manner. Surprisingly, the antifungal potential on adhesion was similar between both species, indicating the potential of (MeOPhSe)2 as a promising antifungal drug against different Candida infections. Conclusion: Overall, we demonstrated the potential of (MeOPhSe)2 as an effective antifungal drug against the virulence factors of Candida species.
Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma
(Elsevier) Vieira, Paula Fonseca Antunes; Jesus, Viviane Paula dos Santos; Cândido, Marcela Aparecida; Pacheco-Soares, Cristina; Castilho, Maiara Lima; Raniero, Leandro José
Background: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nanomaterials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the importance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker molecule Methods: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitro Results: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression properties Conclusion: The findings point to ideal properties for detecting EGFR receptor overexpressed cells.
Modelo de florestas aleatórias para identificar falsos positivos na triagem de fibrose cística com dados artificiais
(Grupo MultiAtual Educacional) Custódio, Paulo Rogério Siqueira; Oliveira , Virginia Klausner de; Ogawa, Guilherme Maerschner; Almeida, Rainara Moreno Sanches de
A Fibrose Cística é uma doença letal que é caracterizada por infecções crônicas no pulmão, insuficiência pancreática e elevados níveis de cloro no suor, essa doença é causada pela mutação no gene do Regulador de Condutância Transmembrana da Fibrose Cística (CFTR), essa doença faz com que o organismo produza secreções espessas e viscosas que obstruem os pulmões, pâncreas e no ducto biliar (RIBEIRO ROSA e colab., 2008). Esta doença faz parte do programa de Triagem Neonatal Brasileiro, sendo triada através da quantificação do Tripsinogênio Imunorreativo (IRT), entretanto essa tripsina apresenta um elevado índice de resultados falsos positivos, isso faz com que seja necessário um outro teste para confirmação do diagnóstico. Sabe-se pela literatura que existem fatores que influenciam na alteração da tripsina como a etnia por exemplo devido a doença ser em sua grande maioria na população caucasiana. Então este trabalho propõe a utilização de técnicas de florestas aleatórias para conseguir detectar a probabilidade de um resultado ser falso positivo analisando as informações
Analysis of Movements in Spinal Cord Hemisection Treatment with Amniotic Membrane – Preclinical Study
(Scientific Research Publishing Inc.) Neves, Marcele Florêncio das; Fonseca, Joyce Lilian Reis da; Carvalho, Paula Cristina Simões de; Pereira, Aline Priscila Campos; Polisel, Evandro Henrique Pintor; Proglhof, Stephanie de Medeiros Passos; Sant ́Anna, Luciana Barros; Lima, Mário Oliveira; Arisawa, Emília Ângela Lo Schiavo
Aims: This study aimed to evaluate the efficacy of Amniotic Membrane application in rats with Spinal Cord Injury induced by transverse hemisection using kinematic analysis and Sciatic Functional Index. Study design: True experimental research design. Place and Duration of Study: Instiuto de Pesquisa e Desenvolvimento (IP&D) of Universidade do Vale do Paraíba (UNIVAP), between September 2016 and December 2017. Methodology: Fifteen adult male rats were used, allocated into three equal groups: Control (the spinal cord injury and Amniotic Membrane application were simulated), Lesion (spinal cord injury not treated), Amniotic membrane (spinal cord injury treated by amniotic membrane). All animals underwent surgical procedures. A transverse hemisection was performed in groups Lesion and Amniotic Membrene. A fragment of the biomaterial was applied in group AM covering the hemisection area. Results: Sciatic Functional Index and motion analysis were performed by comparing images taken at pre- and postoperative time at 7, 14, 21, and 28 days. The kinematic analysis showed a significant difference between groups Control and Lesion at 7 days (p = 0.023) and 14 days (p = 0.015), and between groups Lesion and Amniotic Membrane at 14 days (p = 0.039), comparing the postoperative periods. The Sciatic Functional Index revealed significant differences between Groups Control and Lesion at 7 (p = -0.002), 14 (p = 0.003), and 21 days (p = 0.009), between Groups Control and Amniotic Membrane at 7 (p = 0.014), 14 (p = 0.007), and 28 days (p = 0; 013), and between Groups Lesion and Amniotic Membrane only at 14 days (p = 0.039). Conclusion: Application of amniotic membrane in spinal cord hemisection in rats induced gait recovery and improvement in SFI compared to the untreated group.
Estresse oxidativo em células SH-SY5Y diante da exposição ao peróxido de hidrogênio
(CDRR Editors) Zabeu, Antonieta Marques Caldeira; Rossato, Rafaella Carvalho; Pacheco-Soares, Cristina; Silva, Newton Soares da
O estresse oxidativo é consequência da formação excessiva de espécies reativas de oxigênio geradas normalmente pelo metabolismo celular. Essa produção excessiva ocasiona a perda de capacidade de defesa e de reparo, levando a danos nas biomoléculas (DNA, lipídios, proteínas) celulares. Quando estes danos não são reparados, acabam comprometendo o funcionamento da célula, levando-a a morte por apoptose ou necrose. O estresse oxidativo está associado à morte de células neurais como sendo uma das principais causas de doenças neurodegenerativas, incluindo doença de Alzheimer e doença de Parkinson. O objetivo deste estudo é verificar a ação do peróxido de hidrogênio sendo um modelo de estresse oxidativo em células de neuroblastoma humano. Busca-se analisar a partir de qual concentração ocorre um estresse significante nas células e, em conjunto, verificar esse limiar de concentração em diferentes tempos de ação nas células. Os experimentos realizados compreenderam as análises de atividade mitocondrial obtido por meio do teste de corante tetrazólio (MTT), teste de viabilidade celular utilizando o ensaio de azul de tripano, obtendo-se assim dados quantitativos e qualitativos. Foi possível observar que, a partir da concentração de 0,2 mM durante um intervalo de tempo de 2 horas houve uma diminuição significativa no número de células viáveis em relação ao grupo controle. E, acima dessa concentração, em relação ao tempo, ocorreram mais danos celulares.
Does the composition of toothpaste affect the tooth resistance to erosion/abrasion processes?
(CDRR Editors) Luz, Bruna Ribeiro; Silva, Gustavo Henrique Faria; Silva, Marcelo Henrique Pereira Marques da; Laurindo, Vinicius Silva; Seefeldt, Vanessa Borelli; Nahórny, Sidnei; Soares, Luís Eduardo Silva
This study aimed to evaluate the tooth after brushing with toothpaste containing or not active compounds and with different fluoride concentrations after erosion to establish and compare the effectiveness of each dentifrice in its use. Enamel, E (n=36) and dentin, D (n=36) bovine specimens were treated with artificial saliva (AS - control), fluoridated dentifrice (FD), 8% arginine (AR), and calcium silicate (CS). The samples (n=72) were subjected to cycles of demineralization (orange juice) followed by remineralization (saliva) and then tooth brushing (AR, FD and CS). The above cycle was repeated 3´/day for five days. Micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF), roughness testing and scanning electron microscopy (SEM) were performed. The mean of roughness values (Ra, μm) were E-AS, 0.20; E-FD, 0.15; E-AR, 0.18; E-CS, 0.18; D-AS, 0.31; D-FD, 0.30; D-AR, 0.37; D-CS, 0.44. The SEM images showed a clear loss of tooth substance in AS and FD treatments. A significant positive mineral variation was observed on the dentin after brushed with AR (p<0.05). The FD dentifrice minimized the erosive effects of the orange juice. Arginine and calcium silicate could improve dental protection by the deposition of a surface layer of deposits. Different active compounds resulted in diverse degrees of protection regarding the type of substrate. The high concentration of fluoride and the inclusion of active compounds improves the dentifrice protection level.
Estudos ambientais para avaliação das respostas fisiológicas da espécie rapanea ferruginea submetidas a diferentes condições de incidência solar por espectroscopia no infravermelho com transformada de Fourier (FTIR-ATR)
(Univap) Lins, Evandro Faria; Aquino-Silva, Maria Regina de; Sakane, Kumiko Koibuchi
Com os avanços da tecnologia, a utilização de programas de computador se tornou cada vez mais presente nas pesquisas científicas. A espectroscopia no infravermelho com Transformada de Fourier (FTIR-ATR), aliada à análise computacional, é uma importante técnica empregada em estudos ambientais para caracterização de sistemas biológicos. Essa técnica estuda as ligações químicas presentes nas moléculas ao interagirem com a radiação na faixa do infravermelho do espectro eletromagnético (10-6 a 10-3m) transformando-se em energia vibracional, representada graficamente na forma de um espectro infravermelho. Este estudo objetivou a análise qualita e quantitativa de lignina, clorofila e amido em amostras de folhas de Rapanea ferruginea (nome popular: capororoca) sujeitas a diferentes condições de incidência solar. Para estabelecimento das diferentes condições de incidência solar foram confeccionadas quatro armações em madeira forradas com tela sombrite nas porcentagens de 30%, 50%, 70% e 85% de sombreamento. As mudas foram divididas em cinco grupos, sendo cada grupo disposto sob uma armação, exceto um que foi disposto a pleno sol (sem armação). A coleta das amostras foi realizada após decorrido um mês do início do experimento. Os espectros infravermelhos das amostras foram obtidos na faixa de 4.000 a 450cm-1 . As razões de absorbância entre as bandas de absorção do anel aromático (1.609cm-1 ), C-N-C (1.159cm-1 ) e C-O (1.034cm-1 ) com a banda de estiramento de C-H (2.917cm-1 ) foram consideradas para determinar as quantidades de lignina, clorofila e amido, respectivamente. A partir da quantificação desses três constituintes, a condição mais favorável ao desenvolvimento da planta é a de 70% de sombreamento, seguida pela de 50% de sombreamento, 30% de sombreamento, 85% de sombreamento e por último é a de pleno sol.

Navegar

Submissões Recentes