Produção acadêmica-PPGEB

URI Permanente para esta coleção

https://repositorio.univap.br/handle/123456789/83

Navegar

Agora exibindo 1 - 20 de 77

Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries
(Elsevier) Terra-Garcia, Maíra; Souza, Cheyenne Marçal de; Gonçalves, Nathalia Maria Ferreira; Pereira, André Henrique Correia; Barros, Patrícia Pimentel de; Borges, Alessandra Bühler; Miyakawa, Walter; Ferreira-Strixino, Juliana; Junqueira, Juliana Campos
Photodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm2) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log10 (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissues.
Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study
(Elsevier) Vitorio, Gabrielle dos Santos; Almeida, Rainara Moreno Sanches de; Pinto, Juliana Guerra; Fontana, Letícia Corrêa; Ferreira-Strixino, Juliana
Gliosarcoma is an aggressive brain tumor. Photodynamic Therapy (PDT) is a treatment that can be used for various cancers of the CNS. The aim of this study was to analyze the effects of PDT with Photodithazine (PDZ) in the treatment of gliosarcoma, using 9 L/lacZ cells and serial concentrations of 200 μg/mL to 3.1 μg/mL of PDZ. The samples were divided into two groups: dark and light (10 J/cm2). The PDZ was internalized along all the cytoplasmic extension. Viability tests demonstrated a reduction in viable cells after PDT. The production of ROS was concentration-dependent and PDZ was found in mitochondria and lysosomes, presenting a discrete connection with α-tubulin. However, this structure is likely damaged, evidenced by changes in the morphological analysis. Thus, according to the parameters of this study, PDZ proved to be an interesting PS in PDT for the treatment of gliosarcoma, with the inherent limitations of an in vitro study.
Diphenyl diselenide suppresses key virulence factors of Candida krusei, a neglected fungal pathogen
(Taylor & Francis) Silva, Bruna Graziele Marques da; Pinto, Ana Paula; Passos, Juliene Cristina da Silva; Rocha, João Batista Teixeira da; Silva, Carlos Alberto; Costa, Maricilia Silva
Candida krusei is a candidiasis etiological agent of relevance in the clinical setting because of its intrinsic resistance to fluconazole. Also, it has opened up new paths in the area of alternative therapeutic techniques. This project demonstrated the effects of diphenyl diselenide (PhSe)2 and p-cloro diphenyl diselenide (pCl-PhSe)2, two organochalcogen compounds, on relevant virulence factors for the early stage of the C. krusei host interaction and infection process. Both com- pounds inhibited adherence of C. krusei to both polystyrene surfaces and cervical epithelial cells and biofilm formation; the structure of the biofilm was also changed in a dose-dependent man- ner. In addition, both compounds inhibited C. krusei growth, but (PhSe)2 significantly increased the time duration of the lag phase and delayed the start of the exponential phase in growth kinetics. (PhSe)2 has more potential antifungal activity than (pCl-PhSe)2 in inhibiting the adher- ence to epithelial cells, biofilm formation, and growth of C. krusei.
Análise do comportamento eletromiográfico e da força durante a fadiga do musculo bíceps braquial
(Universidade do Vale do Paraíba) Souza, Gabriela Aparecida da Silveira; Macedo, Humberto Gimenes; Klausner, Virginia; Cezarini, Marina Vedelago; Lemos, Sergio Luiz; Nascimento Filho, Alexandre Alves do; Corrêa, Marina; Spinelli, Bruna Moreira de Oliveira; Barbaroto, Douglas; Pinto, Ana Paula; Lima, Mario; Lopes-Martins, Rodrigo; Lima, Fernanda Púpio Silva
A fadiga muscular é definida como a incapacidade de manter a contração muscular e é ocasionada por alterações bioquímicas que modificam a mecânica da contração muscular, resultando em redução da performance atlética. O objetivo deste estudo foi avaliar o comportamento mioelétrico e a força de indivíduos hígidos durante a fadiga do músculo bíceps braquial. O estudo foi composto por 13 voluntários do sexo masculino com idade entre 20 e 30 anos (25±3,7). Para a indução da fadiga muscular foram realizadas três Contrações Isométricas Voluntárias Máximas (CIVM) com duração de 50 segundos e intervalo de 50 segundos, utilizando um dinamômetro computadorizado acoplado ao eletromiógrafo de superfície. Durante a CIVM foi avaliado o sinal eletromiográfico e a força. Foi possível observar nos resultados uma queda da força muscular e dos parâmetros avaliados por meio da eletromiografia durante a fadiga muscular. A partir da regressão linear dos dados obtidos por meio da eletromiografia e dinamometria foi possível obter o coeficiente angular da reta para cada teste (Teste 1, Teste 2 e Teste 3), nota-se que houve queda de todos os parâmetros avaliados por meio da eletromiografia de superfície e da força muscular, entretanto não houve diferença estatística entre os testes, demonstrando similaridade do comportamento do sinal entre os testes. Conclui-se, portanto, que os parâmetros eletromiográficos analisados (frequência média, frequência mediana e RMS) e a força apresentam um decréscimo durante a fadiga muscular induzida por meio da CIVM.
Efficiency of Antimicrobial Photodynamic Therapy with Photodithazine® on MSSA and MRSA Strains
(MDPI) Souza, Beatriz Müller Nunes; Pinto, Juliana Guerra; Pereira, André Henrique Correia; Miñán, Alejandro Guillermo; Strixino, Juliana Ferreira
Staphylococccus aureus is a ubiquitous and opportunistic bacteria associated with high mortality rates. Antimicrobial photodynamic therapy (aPDT) is based on the application of a light source and a photosensitizer that can interact with molecular oxygen, forming Reactive Oxygen Species (ROS) that result in bacterial inactivation. This study aimed to analyze, in vitro, the action of aPDT with Photodithazine® (PDZ) in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) strains. The strains were incubated with PDZ at 25, 50, 75, and 100 mg/L for 15 min and irradiated with fluences of 25, 50, and 100 J/cm2. The internalization of PDZ was evaluated by confocal microscopy, the bacterial growth by counting the number of colony-forming units, as well as the bacterial metabolic activity post-aPDT and the production of ROS. In both strains, the photosensitizer was internalized; the production of ROS increased when the aPDT was applied; there was a bacterial reduction compared to the control at all the evaluated fluences and concentrations; and, in most parameters, it was obtained complete inactivation with significant difference (p < 0.05). The implementation of aPDT with PDZ in clinical strains of S. aureus has resulted in its complete inactivation, including the MRSA strains.
Fotobiomodulação no tratamento de neuralgia pós-herpética: caso clínico
(Revista Univap) Silva, Erick Giovanni Reis; Arisawa, Emilia Angela Lo Schiavo; Martins, Rodrigo Alvaro Brandão Lopes; Gomes, Raimundo Nonato Silva; Nicolau, Renata Amadei; Pinto, Ana Paula
O presente caso clínico teve por objetivo avaliar a ação da fotobiomodulação (PBM) no tratamento de paciente com diagnóstico de Neuropatia Pós-Herpética (NPH), visando o alívio da dor e a melhora da qualidade de vida. Para a PBM utilizou-se um cluster com três lasers de GaAlAs, com os parâmetros: 660 nm, 100 mW, irradiação pontual, 40 s/ponto, 4 J/cm2 por ponto e total de 10 sessões. Para aferição da dor foram utilizadas a Escala Visual Analógica da Dor (EVA) e Algometria, e a qualidade de vida, com aplicação do Questionário de Avaliação da Qualidade de Vida -SF-36. Os dados obtidos foram submetidos à análise descritiva e ao teste estatístico T pareado, com nível de significância de 5% (p<0,05). Os resultados deste estudo demonstraram redução da dor quando comparadas a 1ª à 10ª sessão, tanto pela EVA quanto pela Algometria. Com relação à análise da qualidade de vida, foi observada uma melhora significativa em todos os oito domínios avaliados. Portanto, conclui-se que a PBM foi eficaz no tratamento da NPH visto que houve redução da dor e melhora na qualidade de vida. Esses resultados são importantes e podem nortear novas pesquisas clínicas que objetivem o emprego da PBM no tratamento da NPH.
Effect of water and alkali on purification bacterial cellulose membrane from Kombucha
(CDRR Editors) Sousa, Letícia Pereira dos Santos Barbosa de; Leite, Priscila Maria Sarmeiro Correa Marciano; Vieira, Angela Aparecida; Faria, Anderson Carlos; Vieira, Lucia
Bacterial cellulose membrane (BCM) is a biomaterial synthesized by bacteria of the genus Gluconocetobacter hansenii with a higher degree of purity than plant cellulose. The commonly used raw material for manipulating bacterial cellulose is kombucha, a beverage consumed by a vast population around the world that promises health benefits. The beverage is composed of tea species Camellia sinenses and a carbon source, refined sucrose, and a starter culture of bacteria and yeast with 10% fermented tea (starter tea) to activate the fermentative process. The Kombucha’s bacterial cellulose membranes (KBCM) are formed over 7 to 10 days on the surface of the fermented product and have the appearance of a gelatinous membrane, this being the by-product of interest. In this work, the objective was to obtain the membrane composed of cellulose via Kombucha and purify it to obtain crystalline cellulose. The purification was performed with distilled water and 0.5M NaOH sodium hydroxide solution to remove residues from the fermentation, successfully removing sugars and bacteria. At the end of the experiments, a lighter film was obtained with coloration close to white, and comparative analyses were performed to verify the structural chemical composition, crystallinity, and morphology of the samples by techniques FTIR, DRX, and SEM, respectively. Then, once the biomaterial was purified, the range of applications expanded to several products to meet the biomedical area, sustainable packaging, and even the fashion industry.
Intranasal photobiomodulation therapy for COVID-19-related olfactory dysfunction: A Brazilian multicenter case series
(ELSEVIER) Soares, Luís Eduardo Silva; Guirado, Milena Miranda Goulart; Berlingieri, Giuseppina; Ramires, Marcia Christina Camargo Hernandes; Lyra, Luciana Aparecida de Oliveira P.; Teixeira, Inessa Solek; Oliveira, Patrícia Costa; Tateno, Ricardo Yudi; Palma, Luiz Felipe; Campos, Luana
Olfactory dysfunction is commonly seen in COVID-19 patients; however, little is known about the pathophysiology and management. The present study aimed to report a series of cases in which three protocols of intranasal photobiomodulation therapy (PBMT) were used for COVID-19-related olfactory dysfunction. Irrespective of the PBMT protocol, olfaction recovery was noted in all cases but with varying degrees of improvement. Although intranasal PBMT seems to be a promising therapeutic modality, more research is needed to better define effectiveness.
Neuroprotective effects of taurine on SH-SY5Y cells under hydrocortisone induced stress
(CDRR Editors) Rossato, Rafaella Carvalho; Granato, Alessandro Eustaquio Campos; Moraes, Carlos Dailton Guedes de Oliveira; Salles, Geisa Nogueira; Soares, Cristina Pacheco
Alzheimer's disease (AD) is the most common, progressive and irreversible neurodegenerative disorder, characterized by memory loss, cognitive impairment and behavioral abnormalities. Although there is no cure, several study strategies seek to elucidate mechanisms of the disease. Recent studies address the benefits of taurine. Thus, the present study aims to analyze neuroprotective effects of taurine in human neuroblastoma (SH-SY5Y), using an in vitro experimental model of oxidative stress induced by hydrocortisone. This work showed for the first time that taurine can promote neuroprotection in SH-SY5Y under oxidative stress caused by hydrocortisone. Cell viability was evaluated using crystal violet and the evaluation of cell morphology was performed by scanning electron microscopy (SEM). The viability of SH-SY5Y pre-treated with taurine and stressed with hydrocortisone was preserved, compared to the stressed only group, which was also morphologically observed. Therefore, taurine can represent a considerable therapeutic candidate in the prevention of neurodegenerative diseases, such as AD.
Gene serpina1: potencial marcador diagnóstico em carcinomas papilíferos da tireoide
(REVISTA UNIVAP) Santos, Joyce Nascimento; Queiroz, João Paulo da Silva; Medeiros Neto, Lázaro Pinto; Santos, André Bandiera de Oliveira; Canevari, Renata de Azevedo
As neoplasias tireoidianas são o principal tipo de malignidade endócrina, sendo atualmente consideradas um problema de saúde pública com aumento constante em sua incidência nos últimos anos. A descoberta de marcadores moleculares que possam ser aplicados na rotina clínica em conjunto com a biópsia por aspiração com agulha fina (PAAF) pode propiciar um diagnóstico mais preciso e consequentemente um tratamento mais eficiente para o paciente. O objetivo desse estudo foi avaliar se o gene SERPINA1 pode ser considerado um marcador diagnóstico em carcinomas de tireoide. Foi realizada a análise de expressão gênica pela RT-qPCR, em 32 amostras de tecido tireoidiano, sendo 13 bócios, 11 carcinomas papilíferos, quatro carcinomas foliculares e quatro tecidos não tumorais de tireoide. A expressão aumentada do gene SERPINA1 foi observada nas amostras de carcinoma papilífero em comparação com amostras de bócio (P=0.0319) e com as amostras de carcinoma folicular (P=0.0430). Não foi observada expressão diferencial significativa entre amostras de carcinoma folicular com as amostras de bócio (P= 0.5329). Os resultados da análise de expressão gênica sugerem que o gene SERPINA1 pode ser considerado um marcador diagnóstico em carcinomas papilíferos de tireoide.
Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes
(MDPI) Pereira, André Henrique Correia; Marcolino, Luciana Maria Cortez; Pinto, Juliana Guerra; Strixino, Juliana Ferreira
Cutaneous leishmaniasis (CL) is a neglected disease prevalent in tropical countries with the ability to cause skin lesions. Photodynamic therapy (PDT) represents a specific and topical option for the treatment of these lesions. This study evaluated the response of macrophages infected with L. braziliensis and L. major to PDT with curcumin. Curcumin concentrations were evaluated in serial dilutions from 500.0 to 7.8 μg/mL using LED (λ = 450 ± 5 nm), with a light dose of 10 J/cm2. The Trypan blue viability test, ultrastructural analysis by scanning electron microscopy (SEM), mitochondrial polarity by Rhodamine 123 (Rho 123), curcumin internalization by confocal microscopy, and counting of the recovered parasites after the PDT treatment were performed. The lowest concentrations of curcumin (15.6 and 7.8 μg/mL) presented photodynamic inactivation. Cell destruction and internalization of curcumin in both macrophages and intracellular parasites were observed in microscopy techniques. In addition, an increase in mitochondrial membrane polarity and a decrease in the number of parasites recovered was observed in the PDT groups. This study indicates that PDT with curcumin has the potential to inactivate infected macrophages and might act as a basis for future in vivo studies using the parameters herein discussed.
Plasmatic and salivary biomarkers for early diagnosis of Autism Spectrum Disorder: a systematic review
(CDRR Editors) Pinto, Mayara Moniz Vieira; Arisawa, Emília Angela Lo Schiavo; Martins, Rodrigo Alvaro Brandão Lopes; Raniero, Leandro José
Autistic Spectrum Disorder (ASD) is considered a neurological development disorder characterized by different degrees of deficit in communication, social interaction, and learning, accompanied by repetitive and stereotyped patterns of behavior. ASD diagnosis is extremely complex due to the still unknown etiopathology, the diversity of symptoms presented by the individuals, and it is carried out solely from clinical observations of the individual's behavior. This study aims to review the main plasma and salivary biomarkers currently studied for the early diagnosis of ASD. For this systematic literature review, we used the online data directory and database “Google Scholar” and “Publish Medliner” (PubMed), respectively, with the descriptors: “Autism”, “Biomarker”, “Diagnostic”, “Saliva”, and “Plasma”. We selected 564 studies in PubMed and 185 in Google Scholar, by screening the titles. After reading the abstracts, we excluded 647 studies, either due to irrelevance or because they were review articles, genetics studies or did not use plasma or saliva samples. The remaining 102 original studies were evaluated in full, and 83 were excluded. Thus, nineteen complete articles that met the inclusion criteria were included in the qualitative analysis. Results identified Cortisol, glutamate/GABA, glutathione, Lipid peroxidation, markers of oxidative stress, mitochondrial dysfunction, and pro-inflammatory cytokines, especially IL-6, as the main plasma and salivary biomarkers currently studied for the early diagnosis of ASD. However, considering that several results were controversial and inconclusive, further studies are needed to validate specific biomarkers as diagnostic tools. The current findings encourage studies that are controlled, multicentric, prospective, and of greater diagnostic precision.
Effect of serial photodynamic therapy with curcumin on Leishmania braziliensis and Leishmania amazonensis promastigotes
(CDRR Editors) Maciel, Lucas Tobias Rodrigues; Marcolino, Luciana Maria Cortez; Maciel, Fernanda Bueno Sant’Anna Pereira; Pinto, Juliana Guerra; Ferreira-Strixino, Juliana
Photodynamic Therapy (PDT) consists of using a light source and a photosensitive drug at an appropriate wavelength and molecular oxygen to trigger cell death through the production of reactive oxygen species. Because it is a localised therapy, PDT is shown to be ideal for skin diseases. American cutaneous Leishmaniasis (ACL) is a highly prevalent protozoan disease worldwide that presents different clinical evolutions and may result in ulcerations and disfiguring lesions on the skin and cartilage. This study was aimed at evaluating the effect in vitro of PDT applied serially using curcumin as a photosensitiser. For this, a concentration of 125 µg.mL-1 of curcumin was used on Leishmania braziliensis and Leishmania amazonensis strains, with a light fluence of 10 J.cm-2 and irradiance of 110 mW.cm-2. The tests done were viability analysis by trypan blue exclusion test, analysis of photosensitizer (PS) internalization by confocal microscopy and morphological alterations by May-Grunwald/Giemsa staining. We observed that there was internalisation of the PS before the first and second application of PDT, with L. braziliensis and L. amazonensis strains mortality of 92% and 82% respectively, after the second application, and induction of alterations in the structural conformation, such as cell size and non-evidence of nucleus and flagellum, demonstrating that PDT was effective. We conclude that serial PDT was effective in inducing the mortality of promastigotes forms of L. braziliensis and L. amazonensis in vitro, thus highlighting its potential for the treatment of leishmaniasis.
Experiences of family members of children diagnosed with autism spectrum disorder
(Revista Gaúcha de Enfermagem) Magalhães, Juliana Macêdo; Rodrigues, Thalia Alves; Rêgo Neta, Marly Marques; Damasceno, Carolinne Kilcia Carvalho Sena; Sousa, Kayo Henrique Jardel Feitosa; Arisawa, Emília Ângela Lo Schiavo
Objective: To describe, in the mothers’ perception, the experiences lived by families in the care of children with autism spectrum disorder. Method: Qualitative study, carried out with 20 mothers of children diagnosed with autistic disorder accompanied by an institution in Teresina-Piauí, Brazil. Semi-structured interviews were conducted between February and March 2019 and subjected to content analysis. Results: Five central ideas related to the stages experienced by family members after the diagnosis were identified, ranging from denial to acceptance. Family members and caregivers experience feelings of sadness and mourning for the discovery of the impossibility of curing the syndrome, revealing the need for care for this family.The search for help and adaptations of the routine are constant experiences. Conclusion: Caring for children who live with autistic disorder involves learning ranging from structural to emotional aspects, such as dealing with limitations and impossibility of cure, pointing out to the need for family care.
Gold nanoparticles associated with temozolomide for glioblastoma Multiforme Treatment
(CDRR Editors) Gialluca, Vanessa Dias; Lima, Vitor Gabriel Poli de; Caixeta, Aloísio; Castilho, Maiara Lima; Raniero, Leandro José
Malignant neoplasms represents a group of diseases that features, as a characteristic, the genetic differentiation of the original tissue, leading to the disordered growth of cells, invading normal tissues and organs. Among the most aggressive tumors, Glioblastoma Multiforme has a mortality rate around 95% and survival’s average of 15 months, even though all treatment available. Temozolomide (TMZ) is the chemotherapeutic drug so far tested and approved with the highest response in this tumor sub-type and must be associated to other treatments to achieve better results. Thus, the purpose of this work was to evaluate the performance of this therapeutic modality with gold nanoparticles (AuNPs) and also combined with radiotherapy. TMZ hydrolysis was characterized at different pH and the chemical changes on molecular structure was determined via Fourier Transform Infrared Spectroscopy (FT-IR). The treatment performance was verified in vitro test using TMZ, TMZ plus AuNPs and associated with radiotherapy. The TMZ concentrations were varied from 0 (control group) to 1000µM, combined with AuNPs from 0 (control group) to10¹⁰ nanoparticles per well. The results showed the drug is stable at pH values between 2 to 4, but for pH values close to the physiological or basic medium, degradation is accentuated reaching a rate of 16 %/hour. The changes on molecular structure of TMZ can be observed through the FT-IR spectra, where the release of oxygen in the structure has influence on C=O group. The results of in vitro experiments showed that the highest poor results in the absence of ionizing irradiation. However, for experiments with TMZ and nanoparticles associated to radiotherapy, the performance of the treatment increased. In summary, the AuNPs showed important results under irradiation, revealing the same level of cytotoxicity for the highest TMZ concentration without irradiation. Also, the synergic effect between AuNPs and TMZ was observed under irradiation condition.
Intervenção de musicoterapia nos sinais vitais de crianças com transtorno do espectro autista: Estudo piloto
(CDRR Editors) Magalhães, Juliana Macêdo; Batista, Pedro Venicius de Sousa; Arisawa, Emilia Angela Lo Schiavo
Objetivo: Avaliar os sinais vitais de crianças com transtorno do espectro autista no contexto de intervenção de musicoterapia. Métodos: Estudo piloto experimental de intervenção de musicoterapia, desenvolvido no nordeste do Brasil. Sete crianças com transtorno do espectro autista foram avaliadas, distribuída em três para intervenção de musicoterapia e quatro sem intervenção. Os dados foram analisados utilizando testes não paramétricos. Resultados: As crianças apresentavam média de idade de oito anos, com maioria do sexo masculino. Descritivamente houve redução nos sinais vitais de 3,7% na saturação de O2, diminuição de 3,3 mmHg na pressão arterial sistólica e aumento de 5,0 bpm na frequência cardíaca no grupo que passou por intervenção de musicoterapia. O grupo sem intervenção apresentou aumento médio de 0,5% na saturação de O2 e de 2,5 mmHg na pressão arterial diastólica. Conclusão: Conclui-se que os efeitos da musicoterapia nos sinais vitais foram indicativos de respostas satisfatórias para as crianças com autismo.
Perfil de crianças com transtorno do espectro autista
(CDRR Editors) Magalhães, Juliana Macêdo; Silva, Thalita Monteiro da; Silva, Fabiana da Conceição; Alencar, Mariana de Fátima Barbosa de; Rêgo Neta, Marly Marques; Alencar, Delmo de Carvalho; Arisawa, Emília Angela Lo Schiavo
Introdução: O Transtorno do Espectro Autista, também conhecido como autismo, é caracterizado como uma deficiência comunicativa, com comportamentos repetitivos e áreas restritas de interesse. Para isso, a identificação do perfil de crianças com a síndrome é de suma importância, o qual pode fornecer dados para efetivar políticas públicas. Objetivo: Este estudo tem como objetivo identificar o perfil de crianças com Transtorno do Espectro Autista. Método: Trata-se de uma pesquisa descritiva e exploratória com abordagem quantitativa, realizada em uma Associação de Amigos dos Autistas, na cidade de Teresina, Piauí, Brasil. Incluíram-se no estudo prontuários de crianças que estavam na faixa etária de zero a onze anos de idade atendidas em 2019. Resultados: Prevaleceu o sexo masculino (80,95%), com idade entre 8 a 9 anos (44,4%), Ensino fundamental (1° a 4° serie) (52,4%). 61 (96,82%) possuíam classificação para autismo infantil e utilizavam o tratamento medicamentoso com Risperidona (23/36,5%). A maioria (61/96,8%) realizava terapia individual e apresentava problemas associados à utilização do brinquedo, psicomotricidade, linguagem, socialização e limites. Conclusão: O estudo contribui para que profissionais sejam capazes de perceber as demandas dos autistas e colaborar com o seu melhor atendimento
Reabilitação sensório-motora associada à fotobiomodulação transcraniana em pacientes com lesão do sistema nervoso central
(Revista Univap) Guimarães, Carolina Lobo; Pinto, Ana Paula; Macedo, Humberto Gimenes; Oliveira, Virgínia Klausner de; Lima, Fernanda Púpio Silva; Lopes-Martins, Rodrigo Álvaro Brandão; Lima, Mário Oliveira
As doenças neurológicas geralmente estão associadas a importantes déficits funcionais, dificultando a execução de atividades de vida diária e autocuidado. Pesquisas recentes demonstram efeitos positivos da Fotobiomodulação Transcraniana (FBM-T) para estimulação do tecido cerebral. O estudo teve como objetivo avaliar os efeitos da FBM-T associada ao treinamento aeróbico na função do sistema musculoesquelético de indivíduos com lesões neurológicas crônicas. Participaram deste ensaio clínico duplo-cego 16 voluntários com diferentes lesões do SNC, de ambos os sexos, idade média de 51,9±13,9 anos e marcha preservada. Os voluntários foram divididos aleatoriamente em 2 grupos (Treino FBM-T ativo + treino aeróbico e FBM-T placebo + treino aeróbico). O programa de reabilitação foi realizado em esteira ergométrica, na frequência de 2 vezes semanais e com duração total de 12 semanas. A FBM-T (3 lasers no λ= 660nm e 3 Lasers no λ= 808nm, 100mW, 6J/laser, 60seg/ponto, 36J por ponto, energia total 108J/cm2) foi aplicada em 3 pontos, sendo estes Fz, F7 e F8 segundo o Sistema Internacional 10-20 de EEG. Foram avaliados RMS do músculo reto femoral parético ou de maior comprometimento no pré-tratamento, 8ª semana e 12ª semana. Nota-se um aumento da atividade elétrica do grupo Laser nas coletas de RMS isotônica e isométrica, e redução do recrutamento muscular em ortostatismo quando comparados o início e o final do treinamento. Sugere-se que a FBM-T pode ter sido um fator influente para o aumento do recrutamento muscular no grupo Laser.
Evaluation of hydrogel use in the development of Rapanea ferruginea with water restriction by vibrational Fourier Transform Infrared Spectroscopy (FTIR-UATR)
(SciELO) Pereira, Douglas Cubas; Pupin, Breno; Sakane, Kumiko Koibuchi
This study evaluated the use of hydrogel on the development of Rapanea ferruginea under water restriction through Vibrational Fourier Transform Infrared Spectroscopy (FTIR-UATR). Seedlings of approximately 30 cm height were transferred in pots with 3 L of soil. The group of seedlings was separated into 5 different triplicate treatments according to the amount of hydrogel, as follows: H1 (25%), H2 (20%), H3 (15%), H4 (10%) and S (control without hydrogel). The applied hydrogel was mixed homogeneously with the soil. All treatments were irrigated with 40 mL of water. FTIR spectra were obtained from fresh leaves collected during 13 months of monitoring. The correlation of the hydroxyl (water) band with the main biomolecules between treatments with the control was evaluated using the Mann-Whitney test (p<0.05). The biomolecule bands were subjected to principal component analysis (PCA) and hierarchical clustering analysis (HCA). The results indicate a significant correlation of the water band with the lipid, hemicellulose, cell wall and starch components in the species. The use of hydrogel resulted in a significant difference in the water absorption band in relation to the control group through the Mann-Whitney test and in biomolecules as the HCA and PCA analysis suggested. The best development was observed in groups H4 (10%), H2 (20%) and H3 (15%). The use of hydrogel positively influences the biomolecular development of the Rapanea ferruginea and monitoring is viable by FTIR.
Ação de extrato de folhas de Acmella oleracea (L.) R. K. Jansen em co-cultivo de Staphylococcus aureus e L929 (fibroblastos) simulando processo de celulite infecciosa
(CDRR Editors) Silva, Laís Eduarda; Silva, Carlos Augusto Priante da; Pacheco-Soares, Cristina; Silva, Newton Soares da
Introdução: A bactéria Staphylococcus aureus é o agente etiológico de maior incidência nas infecções de pele, dentre elas a celulite infecciosa. Seu tratamento é contido por antibióticos, porém o uso de plantas medicinais vem sendo utilizadas para tratamento. Acmella oleracea é uma planta da família Asteraceae conhecida como jambu. Objetivos: Avaliar a ação do extrato das folhas de Acmella oleracea em co-cultivo da linhagem celular L929 e bactérias Staphylococcus aureus simulando o processo de celulite infecciosa. Metodologia: Foi realizado primeiramente o teste MTT e Cristal Violeta na linhagem celular L929 incubadas com o extrato do jambu, nas concentrações 500 µg/mL, 1000 µg/mL e 2000 µg/mL. Foi realizado ensaios com Staphylococcus aureus incubado no período de 24 horas com o extrato do jambu nas mesmas concentrações. Resultados: Na avaliação das células L929 no teste MTT houve uma significância na concentração de 1000 µg/mL apresentando uma baixa atividade metabólica em relação as outras concentrações. No teste cristal violeta ocorreu um maior estímulo nas demais concentrações quando comparado ao controle. Na viabilidade da bactéria observou-se uma queda significativa da viabilidade quando comparado ao controle. A concentração de 500 ug/mL foi que apresentou um menor índice de UFC. Ao realizar o teste do co-cultivo L929 - S. aureus após a incubação de 24 horas com diferentes concentrações do extrato, foi possível avaliar que a ação do extrato apresentou uma redução significativa na viabilidade bacteriana e não interferência à viabilidade das células L929. Conclusão: Com estes resultados demonstramos que o extrato de Acmella oleracea apresentou uma atividade antimicrobiana sem interferir na linhagem de fibroblastos L929, sendo assim podendo ser utilizada contra a celulite infecciosa.

Navegar

Submissões Recentes