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Infrared thermography as valuable tool for gynoid lipodystrophy (cellulite) diagnosis
(Springer-Verlag London Ltd.) Lopes‐Martins, Rodrigo Álvaro Brandão; Barbaroto, Douglas Peretta; Barbosa, Elaine da Silva; Leonardo, Patrícia Sardinha; Ruiz-Silva, Carlos; Arisawa, Emília Ângela Lo Schiavo
Cellulite is a morphological alteration of the tegument tissue, directly interfering in self-esteem with etiology and pathophysiology far from being a consensus. Although the visual diagnosis of cellulitis is well known, it does not represent the real pathological condition of the subcutaneous tissue. The aim of the study was to investigate the hypothesis that the more heterogeneous tissue pattern analyzed by infrared thermography, the more severe is the cellulite grade. Forty female participants were selected and 60 thighs were analyzed by clinical anamnesis and infrared thermography. Classical visual analysis was correlated to the tissue heterogeneity measured by thermography. R Spearman’s correlation between visual evaluation and thermography was 0.92. Phototype presented a negative significant correlation of 0.67 with classical visual analysis. In the present study, we presented a simple method based on infrared thermography that can be adopted in any esthetics office with a correlation of 0.92 with the visual classic evaluation, but, besides, may be very helpful to the clinician to decide which treatment will be adopted, i.e., an aggressive and inflammatory approach such as the radiofrequency of shockwave therapy or an anti-inflammatory approach such as photobiomodulation, depending on the inflammatory status of cellulite.
Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases
(Royal Society of Chemistry) Cairo, João Paulo L. Franco; Mandelli, Fernanda; Tramontina, Robson; Cannella, David; Paradisi, Alessandro; Ciano, Luisa; Ferreira, Marcel R.; Liberato, Marcelo V.; Brenelli, Lívia B.; Gonçalves, Thiago A.; Rodrigues, Gisele N.; Alvarez, Thabata M.; Mofatto, Luciana S.; Carazzolle, Marcelo F.; Pradella, Jose Geraldo da Cruz; Leme, Adriana F. Paes; Leonardo, Ana M. Costa; Neto, Mário Oliveira; Damasio, André; Davies, Gideon J.; Felby, Claus; Walton, Paul H.; Squina, Fabio M.
Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in lignocellulose degradation, suggesting hereto unknown enzymatic activities in their digestome. Herein, we demonstrate that an ancient redox-active enzyme encoded by the lower termite Coptotermes gestroi, a Cu/Zn superoxide dismutase (CgSOD-1), plays a previously unknown role in plant biomass degradation. We show that CgSOD-1 transcripts and peptides are up-regulated in response to an increased level of lignocellulose recalcitrance and that CgSOD-1 localizes in the lumen of the fore- and midguts of C. gestroi together with termite main cellulase, CgEG-1-GH9. CgSOD-1 boosts the saccharification of polysaccharides by CgEG-1-GH9. We show that the boosting effect of CgSOD-1 involves an oxidative mechanism of action in which CgSOD-1 generates reactive oxygen species that subsequently cleave the polysaccharide. SOD-type enzymes constitute a new addition to the growing family of oxidases, ones which are up-regulated when exposed to recalcitrant polysaccharides, and that are used by Nature for biomass degradation.
Local envenomation caused by a bioactive peptide fraction of Bothrops jararaca snake venom induces leukocyte influx in the lung and changes in pulmonary mechanics
(Elsevier) Silva, Carlos Alberto; Querobino, Samyr Machado; Silva, Cesar Augusto Melo; Costa, Maricilia Silva; Oliveira, Luis Vicente Franco; Zamuner, Stella Regina
The crude venom of the Bothrops jararaca snake (Bj-CV) is a complex mixture of biologically active proteins that includes a variety of peptides in the low molecular weight fraction (Bj-PF). We investigated how an intramuscular injection of Bj-CV (1.2 mg kg−1) and Bj-PF (0.24 mg kg−1) influenced lung mechanics and lung and muscle inflammation in male Swiss mice 15 min, 1, 6, and 24 h after inoculation. Pressure dissipation against lung resistive components (ΔP1) rose significantly from 1 to 24 h after Bj-CV and 6–24 h after Bj-PF inoculation. Both Bj-CV and Bj-PF increased the total pressure variation of the lung (ΔPtot) 24 h after injection. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Lung static elastance increased significantly after injection in all time periods investigated by Bj-CV and from 6 to 24 h by Bj-PF. Furthermore, intramuscular inoculation of Bj-CV and Bj-PF resulted in an increase in muscle and pulmonary inflammation, as evidenced by an increase in leukocyte influx when compared to the control group. Finally, both Bj-CV and Bj-PF cause acute lung injury, as shown by pulmonary inflammation and decreased lung mechanics. Furthermore, the fact that Bj-PF produces mechanical alterations in the lungs and muscular inflammation implies that non-enzymatic compounds can cause inflammation.
Estimation of the DNA damage in Girardia tigrina (Girard, 1850) after exposure to radiation under extreme conditions
(Brazilian Journals Publicações de Periódicos) Benitz, Tabatha; Oliveira, Matheus Salgado de; Lopes, Karla Andressa Ruiz; Soares, Cristina Pacheco; Morais, Flavia Villaça; Velho, Nádia Maria Rodrigues de Campos
The use of planarians as experimental models has been increased worldwide especially due to their high capacity of tissue regeneration and ease of laboratory maintenance. The current study aims to assess the genetic damage of Girardia tigrina after exposure of stressors in conditions such as: critical temperature, critical pH, hypergravity (4.4G) and low power laser (685nm). 18 specimens of G. tigrina were used, the extreme conditions mentioned were experimental group with 3 specimens each, and 3 for the control group, all specimens were submitted to the Comet Assay after exposure of stressors, and control group with no extreme condition exposure. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation, caused genetic damages to the G. tigrina, for hypergravity and low-power laser stressors the damage was higher in the pharyngeal and post-pharyngeal regions of the animal with a greatest area of comet tail dragging for the post-pharyngeal region. The extreme stimuli of critical temperature and pH, hypergravity and low power laser irradiation caused genetic damages in planarians Brazilian Journal of Development ISSN: 2525-8761 12160 Brazilian Journal of Development, Curitiba, v.8, n.2, p.12159-12177 feb. 2022. G. tigrina, especially in samples from the pharyngeal and post-pharyngeal regions of the flatworms body assessed by the Comet Assay.
Photodynamic effect of protoporphyrin IX in gliosarcoma 9l/lacZ cell line
(Elsevier B.V.) Fontana, Letícia Corrêa; Pinto, Juliana Guerra; Vitorio, Gabrielle dos Santos; Ferreira, Isabelle; Pacheco-Soares, Cristina; Mamone, Leandro; Ferreira‐Strixino, Juliana
Photodynamic Therapy (PDT) is an oncologic treatment, producing reactive oxygen species (ROS) to induce the death of cancer cells. This study aimed to evaluate the action of PDT on gliosarcoma cells, using protoporphyrin IX as PS by incubation with the precursor aminolevulinic acid (ALA). An LED device was used with a light dose of 10 J/cm². The success of the therapy proved to be dependent on the concentration of ALA, and an incubation time of 4 h required for an effective response. Cell death was prevalent due to necrosis when assessed 18 h post-PDT. ALA proved to be an option to PDT in cells of the 9 L/lacZ, with the protocol tested.
(MeOPhSe)2, a synthetic organic selenium compound, inhibits virulence factors of Candida krusei: Adherence to cervical epithelial cells and biofilm formation
(Elsevier GmbH.) Siqueira, Victor Mendes de; Silva, Bruna Graziele Marques da; Passos, Juliene Cristina da Silva; Pinto, Ana Paula; Rocha, João Batista Teixeira da; Silva, Carlos Alberto; Costa, Maricília Silva
Background: Systemic candidiasis is produced by Candida albicans or non-albicans Candida species, opportunistic fungi that produce both superficial and invasive infections. Despite the availability of a wide range of antifungal agents for the treatment of candidiasis, failure of therapy is observed frequently, which opens new avenues in the field of alternative therapeutic strategies. Methods: The effects of p,p′ -methoxyl-diphenyl diselenide [(MeOPhSe)2], a synthetic organic selenium (organochalcogen) compound, were investigated on virulence factors of C. krusei and compared with its antifungal effects on the virulence factors related to adhesion to cervical epithelial cell surfaces with C. albicans. Results: (MeOPhSe)2, a compound non-toxic in epithelial (HeLa) and fibroblastic (Vero) cells, inhibited the growth in a dose-dependent manner and changed the kinetics parameters of C. krusei and, most importantly, extending the duration of lag phase of growth, inhibiting biofilm formation, and changing the structure of biofilm. Also, (MeOPhSe)2 reduced C. albicans and C. krusei adherence to cervical epithelial cells, an important factor for the early stage of the Candida-host interaction. The reduction was 37.24 ± 2.7 % in C. krusei (p = 0.00153) and 32.84 ± 3.2 % in C. albicans (p = 0.0072) at 20 μM (MeOPhSe)2, and the effect is in a concentration-dependent manner. Surprisingly, the antifungal potential on adhesion was similar between both species, indicating the potential of (MeOPhSe)2 as a promising antifungal drug against different Candida infections. Conclusion: Overall, we demonstrated the potential of (MeOPhSe)2 as an effective antifungal drug against the virulence factors of Candida species.
Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma
(Elsevier) Vieira, Paula Fonseca Antunes; Jesus, Viviane Paula dos Santos; Cândido, Marcela Aparecida; Pacheco-Soares, Cristina; Castilho, Maiara Lima; Raniero, Leandro José
Background: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nanomaterials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the importance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker molecule Methods: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitro Results: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression properties Conclusion: The findings point to ideal properties for detecting EGFR receptor overexpressed cells.
Modelo de florestas aleatórias para identificar falsos positivos na triagem de fibrose cística com dados artificiais
(Grupo MultiAtual Educacional) Custódio, Paulo Rogério Siqueira; Oliveira , Virginia Klausner de; Ogawa, Guilherme Maerschner; Almeida, Rainara Moreno Sanches de
A Fibrose Cística é uma doença letal que é caracterizada por infecções crônicas no pulmão, insuficiência pancreática e elevados níveis de cloro no suor, essa doença é causada pela mutação no gene do Regulador de Condutância Transmembrana da Fibrose Cística (CFTR), essa doença faz com que o organismo produza secreções espessas e viscosas que obstruem os pulmões, pâncreas e no ducto biliar (RIBEIRO ROSA e colab., 2008). Esta doença faz parte do programa de Triagem Neonatal Brasileiro, sendo triada através da quantificação do Tripsinogênio Imunorreativo (IRT), entretanto essa tripsina apresenta um elevado índice de resultados falsos positivos, isso faz com que seja necessário um outro teste para confirmação do diagnóstico. Sabe-se pela literatura que existem fatores que influenciam na alteração da tripsina como a etnia por exemplo devido a doença ser em sua grande maioria na população caucasiana. Então este trabalho propõe a utilização de técnicas de florestas aleatórias para conseguir detectar a probabilidade de um resultado ser falso positivo analisando as informações
Analysis of Movements in Spinal Cord Hemisection Treatment with Amniotic Membrane – Preclinical Study
(Scientific Research Publishing Inc.) Neves, Marcele Florêncio das; Fonseca, Joyce Lilian Reis da; Carvalho, Paula Cristina Simões de; Pereira, Aline Priscila Campos; Polisel, Evandro Henrique Pintor; Proglhof, Stephanie de Medeiros Passos; Sant ́Anna, Luciana Barros; Lima, Mário Oliveira; Arisawa, Emília Ângela Lo Schiavo
Aims: This study aimed to evaluate the efficacy of Amniotic Membrane application in rats with Spinal Cord Injury induced by transverse hemisection using kinematic analysis and Sciatic Functional Index. Study design: True experimental research design. Place and Duration of Study: Instiuto de Pesquisa e Desenvolvimento (IP&D) of Universidade do Vale do Paraíba (UNIVAP), between September 2016 and December 2017. Methodology: Fifteen adult male rats were used, allocated into three equal groups: Control (the spinal cord injury and Amniotic Membrane application were simulated), Lesion (spinal cord injury not treated), Amniotic membrane (spinal cord injury treated by amniotic membrane). All animals underwent surgical procedures. A transverse hemisection was performed in groups Lesion and Amniotic Membrene. A fragment of the biomaterial was applied in group AM covering the hemisection area. Results: Sciatic Functional Index and motion analysis were performed by comparing images taken at pre- and postoperative time at 7, 14, 21, and 28 days. The kinematic analysis showed a significant difference between groups Control and Lesion at 7 days (p = 0.023) and 14 days (p = 0.015), and between groups Lesion and Amniotic Membrane at 14 days (p = 0.039), comparing the postoperative periods. The Sciatic Functional Index revealed significant differences between Groups Control and Lesion at 7 (p = -0.002), 14 (p = 0.003), and 21 days (p = 0.009), between Groups Control and Amniotic Membrane at 7 (p = 0.014), 14 (p = 0.007), and 28 days (p = 0; 013), and between Groups Lesion and Amniotic Membrane only at 14 days (p = 0.039). Conclusion: Application of amniotic membrane in spinal cord hemisection in rats induced gait recovery and improvement in SFI compared to the untreated group.
Estresse oxidativo em células SH-SY5Y diante da exposição ao peróxido de hidrogênio
(CDRR Editors) Zabeu, Antonieta Marques Caldeira; Rossato, Rafaella Carvalho; Pacheco-Soares, Cristina; Silva, Newton Soares da
O estresse oxidativo é consequência da formação excessiva de espécies reativas de oxigênio geradas normalmente pelo metabolismo celular. Essa produção excessiva ocasiona a perda de capacidade de defesa e de reparo, levando a danos nas biomoléculas (DNA, lipídios, proteínas) celulares. Quando estes danos não são reparados, acabam comprometendo o funcionamento da célula, levando-a a morte por apoptose ou necrose. O estresse oxidativo está associado à morte de células neurais como sendo uma das principais causas de doenças neurodegenerativas, incluindo doença de Alzheimer e doença de Parkinson. O objetivo deste estudo é verificar a ação do peróxido de hidrogênio sendo um modelo de estresse oxidativo em células de neuroblastoma humano. Busca-se analisar a partir de qual concentração ocorre um estresse significante nas células e, em conjunto, verificar esse limiar de concentração em diferentes tempos de ação nas células. Os experimentos realizados compreenderam as análises de atividade mitocondrial obtido por meio do teste de corante tetrazólio (MTT), teste de viabilidade celular utilizando o ensaio de azul de tripano, obtendo-se assim dados quantitativos e qualitativos. Foi possível observar que, a partir da concentração de 0,2 mM durante um intervalo de tempo de 2 horas houve uma diminuição significativa no número de células viáveis em relação ao grupo controle. E, acima dessa concentração, em relação ao tempo, ocorreram mais danos celulares.
Does the composition of toothpaste affect the tooth resistance to erosion/abrasion processes?
(CDRR Editors) Luz, Bruna Ribeiro; Silva, Gustavo Henrique Faria; Silva, Marcelo Henrique Pereira Marques da; Laurindo, Vinicius Silva; Seefeldt, Vanessa Borelli; Nahórny, Sidnei; Soares, Luís Eduardo Silva
This study aimed to evaluate the tooth after brushing with toothpaste containing or not active compounds and with different fluoride concentrations after erosion to establish and compare the effectiveness of each dentifrice in its use. Enamel, E (n=36) and dentin, D (n=36) bovine specimens were treated with artificial saliva (AS - control), fluoridated dentifrice (FD), 8% arginine (AR), and calcium silicate (CS). The samples (n=72) were subjected to cycles of demineralization (orange juice) followed by remineralization (saliva) and then tooth brushing (AR, FD and CS). The above cycle was repeated 3´/day for five days. Micro energy-dispersive X-ray fluorescence spectrometry (µ-EDXRF), roughness testing and scanning electron microscopy (SEM) were performed. The mean of roughness values (Ra, μm) were E-AS, 0.20; E-FD, 0.15; E-AR, 0.18; E-CS, 0.18; D-AS, 0.31; D-FD, 0.30; D-AR, 0.37; D-CS, 0.44. The SEM images showed a clear loss of tooth substance in AS and FD treatments. A significant positive mineral variation was observed on the dentin after brushed with AR (p<0.05). The FD dentifrice minimized the erosive effects of the orange juice. Arginine and calcium silicate could improve dental protection by the deposition of a surface layer of deposits. Different active compounds resulted in diverse degrees of protection regarding the type of substrate. The high concentration of fluoride and the inclusion of active compounds improves the dentifrice protection level.
Estudos ambientais para avaliação das respostas fisiológicas da espécie rapanea ferruginea submetidas a diferentes condições de incidência solar por espectroscopia no infravermelho com transformada de Fourier (FTIR-ATR)
(Univap) Lins, Evandro Faria; Aquino-Silva, Maria Regina de; Sakane, Kumiko Koibuchi
Com os avanços da tecnologia, a utilização de programas de computador se tornou cada vez mais presente nas pesquisas científicas. A espectroscopia no infravermelho com Transformada de Fourier (FTIR-ATR), aliada à análise computacional, é uma importante técnica empregada em estudos ambientais para caracterização de sistemas biológicos. Essa técnica estuda as ligações químicas presentes nas moléculas ao interagirem com a radiação na faixa do infravermelho do espectro eletromagnético (10-6 a 10-3m) transformando-se em energia vibracional, representada graficamente na forma de um espectro infravermelho. Este estudo objetivou a análise qualita e quantitativa de lignina, clorofila e amido em amostras de folhas de Rapanea ferruginea (nome popular: capororoca) sujeitas a diferentes condições de incidência solar. Para estabelecimento das diferentes condições de incidência solar foram confeccionadas quatro armações em madeira forradas com tela sombrite nas porcentagens de 30%, 50%, 70% e 85% de sombreamento. As mudas foram divididas em cinco grupos, sendo cada grupo disposto sob uma armação, exceto um que foi disposto a pleno sol (sem armação). A coleta das amostras foi realizada após decorrido um mês do início do experimento. Os espectros infravermelhos das amostras foram obtidos na faixa de 4.000 a 450cm-1 . As razões de absorbância entre as bandas de absorção do anel aromático (1.609cm-1 ), C-N-C (1.159cm-1 ) e C-O (1.034cm-1 ) com a banda de estiramento de C-H (2.917cm-1 ) foram consideradas para determinar as quantidades de lignina, clorofila e amido, respectivamente. A partir da quantificação desses três constituintes, a condição mais favorável ao desenvolvimento da planta é a de 70% de sombreamento, seguida pela de 50% de sombreamento, 30% de sombreamento, 85% de sombreamento e por último é a de pleno sol.
Photobiomodulation assay of muscle cells C2C12 after irradiation with LED device
(CDRR Editors) Lima, Elessandro Váguino de; Pacheco-Soares, Cristina; Silva, Newton Soares da
Introduction:One of the ways that have been observed to reduce musculoskeletal fatigue is the use of protocols for the application of light sources (photobiomodulation) such as low-intensity laser and LED (Light Emitting Diode). Work involving photobiomodulation has shown promising results in strength performance or reduction of muscle fatigue. At the cellular level,photobiomodulation can modulate fibroblasts proliferation, the fixation and synthesis of collagen and procollagen, promote angiogenesis and improving energy metabolism in mitochondria. Compared with laser devices, LED has several advantages, such as beingsmaller, lighter, lower cost, and easier for operation. Objective:The present work objective is to verify if irradiation with LED device (650 nm and 860 nm) in muscle cells C2C12 modify the viability, morphology and cytoskeleton components. Methodology:C2C12 cells line (ATCC CRL -1772) were cultured in 25 cm2bottles at 37ºC under 5% CO2in DMEM. The cells wereirradiated with the light-emitting diodes (LED) device, Sportllux Ultra that consists of 84 LEDs, each individual LED has 8 mW of power, emitting in 660±20 nm (42 LEDs) and 850±20 nm (42 LEDs), and covering anarea (A) of 120 cm2. The power density of delivered light was 5,6 mW/cm2, and the exposure time was 10 minutes, totalizing the fluence of 3,4 J/cm2. Viability assay was performed where the cells were incubated with 100 μL of Crystal Violet (CV) solution and mitochondrial activity assay was evaluated by the colorimetric MTT assay. Nucleus (DAPI) and Cytoskeleton (Rhodamine Phalloidin) fluorescence assay was performed to study the cytoskeleton based on the change in the actin filaments. Results:Our results demonstrate that the synergism of LED irradiation (660nm and 850nm) induced the proliferation of C2C12 cells. The light-emitting diode (LED) device, Sportllux Ultra has a significant effect on C2C12 cell culture. Mitochondrial activity and cell viability showed a significative increase in their activities after irradiation. The microscopy fluorescence observations showed an alignment of cytoskeletal components of C2C12 cells after irradiation.Conclusion:The application of irradiation with the Sportlux Ultra LED device stimulated an increase of energy by mitochondrial activity assay, number of cells by cell viability assay and alignment of cytoskeleton components by fluorescence assay in C2C12line cells. Our results suggest that organizated cytoskeletal actin filaments normally contribute to cell survival and that induced major cell changes in the cytoskeleton that result in cell shape change. These results suggest that the Sport Lux Ultra LEDdevice can help in the repair of tissue injuries and to collaborate to increase of performance in athletes in a faster way
Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii
(Elsevier) Camargo, Larissa de Oliveira; Fontoura, Inglid; Veriato, Thaís da Silva; Raniero, Leandro José; Castilho, Maiara Lima
Background: Multidrug-resistant bacteria are one of the world's biggest health problems; therefore, improving the spectrum of action of antibiotics could be necessary to reverse this situation. Amikacin and silver salts have well-known antimicrobial properties. However, both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized with Amikacin against multidrug-resistant Acinetobacter baumannii. Methods: AgNPs were produced using the bottom-up methodology and functionalized with Amikacin modified by the carbodiimide-based chemistry, forming AgNPs@Amikacin. Susceptibility tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification and the metabolic activity were determined. Results: The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution, with a size of 37.84 nm. The FT-IR spectrum of AgNPs@Amikacin exhibits vibrational modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5 µg/mL. The AgNPs@Amikacin reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations. Conclusions: Results demonstrate a promising development of a new nanodrug with lower concentrations, less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.
Evaluation of the effects of photobiomodulation (808 nm) on pain and quality of life of diabetic neuropathy patients
(CDRR Editors) Leal, Milena Valdinéia da Silva; Lima, Mário Oliveira; Costa, Davidson Ribeiro; Nicolau, Renata Amadei; Ribeiro, Luana Aparecida Pilato; Costa, David Ribeiro; Carvallho, Teresa Marly Teles de; Silva, Kelson Nonato Gomes da; Pessoa, Diego Rodrigues; Arisawa, Emília Ângela Lo Schiavo
Diabetic neuropathy (DN) is one of the main complications of diabetes mellitus (DM), responsible for a high morbimortality rate and burdening public health resources. Photobiomodulation has proven to be effective in relieving pain, reducing the inflammation, and improving vascularization. We report an evaluation of the effectiveness of photobiomodulation for the relief of pain and to improve the quality of life in patients with diabetic neuropathy. A total of 30 diabetic volunteers with DN were randomly divided into three groups: control, photobiomodulation (PBM), and placebo (P-PBM). Those in Group control were instructed to take diabetic neuropathy medication for 30 days. The PBM group received laser photobiomodulation treatment with the following protocol: three sessions per week for 30 days, totaling 12 applications (wavelength, 808 nm; energy density, 12 J/cm²). in the of following nerves: tibial medial plantar, own plantar digital, common plantar digital, superficial fibular, deep fibular, sciatic, saphenous, and common fibular. Patients in the P-PBM Group were submitted to the PBM protocol, but with the device switched off. Before and after applying the therapeutic protocols, all volunteers were subjected to pain evaluations. The results showed a statistically significant difference between the groups (p < 0.05). The PBM group confirmed an analgesic effect directly related to the use of the photobiomodulation, whilst the patients in group control and P-PBM, did not present significant results. The study demonstrated that laser photobiomodulation is effective in significantly reducing pain and improving the quality of life of patients with diabetic neuropathy.
Dentifrícios contendo fitoterápicos em sua composição e seus benefícios: uma revisão de literatura
(CDRR Editors) Lopes, Ludimila Carla Fróes; Soares, Luís Eduardo Silva
Os dentifrícios são produtos de higiene pessoal utilizados na prevenção e no tratamento das doenças prevalentes na cavidade bucal como a cárie dentária e as doenças periodontais. Este estudo trata-se de uma revisão de literatura, com caráter narrativo sobre os dentifrícios contendo fitoterápicos em sua composição e seus benefícios relatados. A revisão de literatura foi realizada com artigos publicados na língua portuguesa e inglesa, encontrados entre os meses de janeiro e agosto de 2021 nas bases de dados online do Scielo, BVS, PubMed e Google Acadêmico. Com as informações selecionadas, foi realizada uma análise narrativa, relacionando os dentifrícios e seus componentes bem como as propriedades de cada fitoterápico. Foram selecionados 22 artigos entre osanos de 1999 e 2021. A adição de fitoterápicos aos dentifrícios potencializa sua ação. As indicações usuais dos fitoterápicos estão associadas no tratamento de infecções, inflamações, odontalgias e processos cicatriciais, dentre outras. A utilização de forma correta dos fitoterápicos fornece uma série de benefícios, vistoque os produtos naturais são de fácil acesso, apresentam baixa toxicidade e biocompatibilidade comprovada cientificamente. Os fitoterápicos presentes nos dentifrícios são opções viáveis no controle e tratamento das afecções bucais, principalmente o biofilme dentário.
Busca de marcadores moleculares preditivos do acometimento de linfonodos em carcinomas papilíferos de tireoide
(Universidade do Vale do Paraíba - UNIVAP) Oliveira, Maria Luiza Lopes de; Costa, João Vitor Alcântara da; Silva, Luana Araujo da; Santos, Joyce Nascimento; Canevari, Renata de Azevedo
O câncer de tireoide é a neoplasia que mais cresce em incidência todos os anos, sendo o carcinoma papilífero de tireoide (CPT) o subtipo mais comum. Em muitos casos estes tumores podem se tornar agressivos, ocasionando metástases linfonodais. A determinação de marcadores moleculares pela análise de expressão gênica é uma importante ferramenta para a determinação do prognóstico destes tumores. Considerando o papel dos genes NEDD9, B3GNT7, PHB, BAD, PAXIP1, PPM1D e PIK3R5 na etiologia de diversos cânceres, este estudo consistiu em verificar pela técnica de RT-qPCR se os mesmos podem ser marcadores moleculares preditivos do acometimento de linfonodos e se estão relacionados ao desenvolvimento destes tumores. No presente estudo não foi observada expressão diferencial significativa dos genes analisados entre os grupos amostrais de CPT versus MCPT (microcarcinomas) e entre os grupos de CPT linfonodo positivo versus CPT linfonodo negativo, indicando que estes genes não estão relacionados ao desenvolvimento tumoral e não podem ser considerados marcadores preditivos de metástases em linfonodos em CPT.
Comparação da mecânica respiratória e performance muscular entre indivíduos curados de SARS-CoV-2 com tratamentodomiciliar e tratamento hospitalar
(CDRR Editors) Moreira, Natália Galvão Rocha; Licurci, Maria das Graças Bastos; Nogueira, Daniel Vilela; Fagundes, Alessandra de Almeida; Costa, Maricilia Silva
Introdução: Após a descoberta feita pelas autoridades da saúde chinesa, identificando o vírus SARS-CoV-2, houve a necessidade de maiores estudos, por se tornar pandemia. Mesmo os infectados por COVID-19 apresentarem sintomas leves ou assintomáticos, observou-se principalmente apresentações clínicas as afecções de trato respiratório e alterações musculares durante o período em que o indivíduo estava infectado, em tratamento ou pós-COVID. Deste modo, o objetivo deste estudo foi avaliar e comparar o comportamento da mecânica respiratória e performance muscular entre os indivíduos pós-COVID-19 que foram hospitalizados e daqueles que receberam tratamento domiciliar. Método: Avaliação de 13 indivíduos, na faixa etária de 25 a 65 anos, divididos em 2 grupos: Grupo 1 - pós diagnóstico de COVID-19 submetidos a internação hospitalar e Grupo 2 - pós diagnóstico de COVID-19 submetidos a tratamento domiciliar. Ambos os grupos fora do período de isolamento, sem risco de contaminação. Os indivíduos foram avaliados por manovacuometria, pico de fluxo expiratório e performance muscular. Dados apresentados em forma de média e desvio padrão, comparados entre os grupos por meio do teste T-Student com nível de significância de 5%. Resultados: houve uma diferença significativa na comparação entre as médias de PIMax do Grupo 1 entre os valores preditos (p=0,04), enquanto as outras comparações obtivemos p>0,05. Conclusão: mesmo com diversas publicações relatando as diferenças na mecânica respiratória e endurance muscular, essas diferenças não foram significativas estatisticamente no nosso trabalho, sendo melhor apresentadas com um maior número de amostra e menor tempo entre a infecção pelo COVID-19 e as avaliações.
Análise dos sinais vitais e do desempenho cardiorrespiratório de pessoas com disfunções neurológicas após realização de um protocolo de reabilitação
(CDRR Editors) Lopes, Taciana; Pinto, Ana Paula; Lemos, Sérgio Luiz; Carolina Lobo Guimarães; Abreu, Élida Goulart de; Machado, Douglas Vinicius de Souza; Lopes-Martins, Rodrigo Álvaro Brandão; Lima, Mário de Oliveira; Lima, Fernanda Pupio Silva
Este estudo teve como objetivo avaliar os sinais vitais e o desempenho cardiorrespiratório (velocidade média durante o treino aeróbico) de pessoas com disfunções neurológicas após realização de um protocolo de reabilitação. Participaram 14 voluntários de ambos os gêneros, entre 18 a 85 anos. Ao longo do protocolo foram coletados os sinais vitais durante o repouso inicial; esforço e recuperação. Foram avaliados parâmetros como: pressão arterial (PA), saturação periférica de oxigênio (SpO2), frequência cardíaca (FC), escala de percepção subjetiva de esforço (BORG) e velocidade média da marcha em um programa de reabilitação cardiorrespiratória durante 12 semanas, totalizando 24 sessões, sendo que cada sessão teve duração de 1 hora. Verificou-se que a frequência cardíaca de recuperação pós exercício reduziu em relação aos esforços, mas não retornou aos valores basais com diferença estatística. Por sua vez, a frequência cardíaca inicial durante o repouso ao longo do tratamento, apresentou diminuição significativa (p<0,05). O desempenho cardiorrespiratório se comportou em fases (fase inicial, intermediária, final), sendo que o resultado mais satisfatório ocorreu na fase final, a partir da 14ª sessão. A FC durante o esforço apresentou um aumento significativo de 6%. Assim, foi possível constatar que após a reabilitação cardiorrespiratória, a FCi diminuiu, ocorreu melhora do desempenho sem grandes variações de FC, PA e SpO2 durante o esforço, demonstrando que o tratamento foi benéfico e seguro para os voluntários com lesão do sistema nervoso central.
Application of mid-infrared vibrational spectroscopy with Fourier transform (FTIR) in quality evaluation in commercial coffees
(CDRR Editors) Fontes, Vitória; Pereira, Douglas Cubas; Lyra, Lucas Ferreira; Sakane, Kumiko Koibuchi
Currently, Brazil is the largest exporter and producer of coffee in the world, and it is the second most consumed beverage in the world, only behind water. In the years 2019 and 2020 it is estimated that the world consumption of coffee was 168.84 million bags of 60 kg, Brazil consumed 20 million bags of coffee, the second-largest consumer in the world, only behind the United States with 25 million bags. The techniques such as infrared spectroscopy has been applied in the food industry, as it is a fast, easy technique, without the need for reagents, free from polluting processes, and capable of analyzing the simultaneous composition of the constituents. The present study aims to analyze the changes in the chemical constituents of Brazilian commercial coffees as a function of shelf life through Fourier transformed infrared spectroscopy (FT-IR) associated with chemometric methods. The experiments were carried out within the expiration date, 6 months, and a year after the expiration date. Spectra were obtained in the range from 4000 to 500 cm-1. The studies of Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were made as discrimination methods. The areas in the region from 2970 to 2830 cm-1 and 1765 to 1720 cm-1 were calculated to analyze the alteration as a function over time. The results suggest that these bands in coffee are sensitive over time and to the storage conditions, promoting changes in aroma and flavor.

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