Produção acadêmica-PPGEB

URI Permanente para esta coleção

https://repositorio.univap.br/handle/123456789/83

Navegar

Agora exibindo 1 - 20 de 154

Transcranial photobiomodulation therapy associated with cardiorespiratory rehabilitation in spastic subjects
(Springer Nature Link) Pinto, Ana Paula; Lemos, Sergio Luiz; Fagundes, Alessandra de Almeida; Neves, Marcele Florêncio das; Martins, Rodrigo Álvaro Brandão Lopes; Silva, Fernanda Pupio; Silva, Mário Oliveira
The objective of this study was to evaluate the effects of cardiorespiratory rehabilitation (CR) and transcranial photobiomodu- lation (tPBM) on exercise tolerance (ET), heart rate variability (HRV), and peripheral muscle activity in individuals with spasticity. Fifteen participants with spasticity were randomly assigned to two groups: the tPBM group (tPBMG) consisted of eight volunteers who underwent tPBM (on mode) and CR, while the control group (CG) consisted of seven volunteers who underwent simulated tPBM (off mode) and CR. The CR program included 12 weeks of treatment, twice a week for one hour, involving aerobic exercises and lower limb strengthening. For tPBM, a cluster with three lasers (λ = 680 nm, 808 nm), with a power of 100 mW/laser and energy of 36 J, applied to the F7, F8, and Fpz points. The following parameters were evalu- ated after 8 and 12 weeks: ET, HRV, and surface electromyography (EMG) of the rectus femoris muscle during orthostasis (ORT), isometric squatting (ISOM), and isotonic squatting (ISOT). Both groups showed a 40% increase in ET for the CG and a 30% increase for the tPBMG. The CG had more pronounced parasympathetic modulation alterations during post-exercise effort and recovery compared to the tPBMG. The EMG results showed that the tPBMG exhibited progressive improvement in muscle activity during ISOM and ISOT, as well as a decrease in the interlimb difference. In conclusion, both CR and tPBMG demonstrated improvements in ET. However, tPBMG specifically showed promising effects on HRV modulation and peripheral muscle electrical activity, providing additional benefits compared to CR alone.
Evaluatin of the effects of photobiomodulation (808 nm) on pain and quality of life of diabetic neuropathy patients
(CDRR Editor) Leal, Milena Valdinéia da Silva; Lima, Mário Oliveira; Costa, Davidson Ribeiro; Nicolau, Renata Amadei; Ribeiro, Luana Aparecida Pilato; Costa, David Ribeiro; Carvalho, Teresa Marly Teles de; Silva, Kelson Nonato Gomes da; Pessoa, Diego Rodrigues; Arisawa, Emilia Angela Lo Schiavo
Diabetic neuropathy (DN) is one of the main complications of diabetes mellitus (DM), responsible for a high morbimortality rate and burdening public health resources. Photobiomodulation has proven to be effective in relieving pain, reducing the inflammation, and improving vascularization. We report an evaluation of the effectiveness of photobiomodulation for the relief of pain and to improve the quality of life in patients with diabetic neuropathy. A total of 30 diabetic volunteers with DN were randomly divided into three groups: control, photobiomodulation (PBM), and placebo (P-PBM). Those in Group control were instructed to take diabetic neuropathy medication for 30 days. The PBM group received laser photobiomodulation treatment with the following protocol: three sessions per week for 30 days, totaling 12 applications (wavelength, 808 nm; energy density, 12 J/cm2). in the of following nerves: tibial medial plantar, own plantar digital, common plantar digital, superficial fibular, deep fibular, sciatic, saphenous, and common fibular. Patients in the P-PBM Group were submitted to the PBM protocol, but with the device switched off. Before and after applying the therapeutic protocols, all volunteers were subjected to pain evaluations. The results showed a statistically significant difference between the groups (p < 0.05). The PBM group confirmed an analgesic effect directly related to the use of the photobiomodulation, whilst the patients in group control and P-PBM, did not present significant results. The study demonstrated that laser photobiomodulation is effective in significantly reducing pain and improving the quality of life of patients with diabetic neuropathy.
Effect of photobiomodulation associated with strengthening pelvic floor muscles in volunteers with urinary incontinence: a randomized, double‐blinded, and placebo‐controlled clinical trial
(Springer) Silva, Aline Lanziloti da; Martins, Rodrigo Alvaro Brandão Lopes; Oliveira, Aissa dos Santos; França, Paula Denise Leite; Pereira, Maria Fernanda Franco; Lima, Mário Oliveira; Lima, Fernanda Pupio Silva
The dysfunctions of the female pelvic floor have a great influence on the quality of life of women, in all areas, social, psy- chological, and sexual. Stress urinary incontinence is the clinical condition in which the woman involuntarily loses urine to efforts, such as coughing and sneezing, causing great embarrassment and affecting her quality of life. The physiotherapeu- tic treatments include muscle strengthening; however, muscle fatigue is present when performing the exercises. Here we investigate the effects of photobiomodulation to prevent muscle fatigue in the pelvic floor in the treatment of stress urinary incontinence, associated with a muscle strengthening exercise protocol. We used an infrared laser (808 nm, 100 mW) and 3 J/point and fluence of 107.1 J/cm2. The application was performed at 3 points on the vaginal introits and at another 3 points inside the vaginal canal cavity for the treatment of stress urinary incontinence associated with strengthening exercises with vaginal cones. Twenty-two volunteers participated in the study, divided into two groups: group 1 (laser therapy + strength- ening) and group 2 (placebo laser + strengthening). In the group 1 quality of life score, the assessment (11.63 ± 4.33) was the highest score at 17 and in the reevaluation (7.81 ± 5.14) the lowest was 0 (p < 0.05). The muscular strength increased considerably (p < 0.05) for group 1, where the vast majority of patients gained more than twice the strength in the pelvic apparatus (8.36 ± 6.65 before X 13.81 ± 8.92 after). The volunteers acquired an increase in the contraction of the muscles of the pelvic apparatus (p < 0.05) (3.45 ± 1.07); after laser application, this number increased considerably (4.27 ± 0.61). Endurance had an increase of almost 50% compared to placebo, demonstrating the resistance gain in the perineal muscles (3.90 ± 2.35 X 5 ± 1.90). We concluded that photobiomodulation treatment showed significant efficacy in relation to muscle fatigue in the pelvic apparatus right after a strengthening program in women with stress urinary incontinence.
Avaliação comparativa da distância percorrida, da função pulmonar e da qualidade de vida após reabilitação cardiorrespiratória com e sem fotobiomodulação transcraniana em indivíduos com espasticidade – Estudo piloto
(CDRR Editor) Pinto, Ana Paula; Lemos, Sergio Luiz; Fagundes, Alessandra de Almeida; Martins, Rodrigo Álvaro Brandão Lopes; Neves, Marcele Florêncio das; Lima, Fernanda Pupio Silva; Lima, Mário Oliveira
O objetivo do estudo foi avaliar os efeitos da reabilitação cardiorrespiratória (RC) isolada e associada a fotobiomodulação transcraniana (Ft) na distância percorrida (DP), função pulmonar e qualidade de vida de indivíduos com espasticidade. Os participantes foram alocados em dois grupos: Grupo fotobiomodulação transcraniana (GF, RC + Ft ativa, n=8) e Grupo placebo (GP, RC + Ft simulada, n=7). A RC consistiu em 12 semanas de tratamento, 2x semana por 1 hora, incluindo exercícios aeróbicos, respiratórios e resistidos de membros inferiores. Para Ft utilizou-se o cluster de laser (λ= 680 nm e 3 Lasers no λ= 808 nm, energia total de 36 J/ponto) nos pontos F7, F8, AFz. Foram avaliadas a DP, espirometria, manovacuometria e SF-36 na avaliação basal e reavaliação com 8 e 12 semanas. A DP aumentou em 165,43 metros e 222,46m em 12 semanas para GF e GP, respectivamente, com diferenças estatísticas intragrupos (p<0,05) e sem diferença intergrupos (p>0,05). Observou-se aumento no índice pico de fluxo expiratório apenas no GF na análise basal x12 semanas (p<0,05). O GF apresentou aumento da PImax e PEmax em 20% (8 semanas) e 15% (12 semanas). No SF-36, a diferença em relação ao basal, verificou-se os domínios aspectos físicos e dor para o GF comportamento de crescimento, e para o GP de decréscimo. Concluiu-se que a DP melhorou no GF e GP pós-intervenção, sem efeito potencializador do desempenho no GF e que a Ft parece ter influenciado na mecânica respiratória do GF e nos aspectos físicos e dor do SF-36.
Análise da performance muscular de atletas submetidos à reconstrução do ligamento cruzado anterior após programa de reabilitação
(CDRR Editor) Nogueira, Daniel Vilela; Lemos, Sergio Luiz; Carvalho Neto, Fernando Azevedo; Lima, Fernanda Pupio Silva; Lima, Mário Oliveira
As lesões ligamentares de joelho vêm sendo amplamente discutidas nos últimos anos, impulsionado pelo elevado número de casos na prática esportiva. O objetivo desse estudo foi comparar o desempenho muscular de atletas que participaram de um programa de reabilitação após reconstrução do ligamento cruzado anterior (LCA), por meio de um software desenvolvido para avaliar parâmetros como pico de torque, trabalho total e potência muscular. Foram avaliados 59 atletas amadores, submetidos a reconstrução do LCA com enxerto do grupo muscular flexor, com 6 meses de reabilitação iniciada imediatamente a cirurgia. A avaliação foi constituída por 5 contrações isocinéticas concêntricas com velocidade constante de 60o/s para flexão e extensão do joelho realizadas no dinamômetro isocinético. Os dados obtidos foram analisados por meio do software Análise da Performance Muscular na Dinamometria Isocinética®, desenvolvido para a realização desse estudo. Os resultados obtidos permitiram observar uma diferença de mais de 10% entre os lados para o pico torque, trabalho total e potência máxima (p<0,05) sendo que o grupo muscular extensor apresentou valores menores para o lado operado. O grupo muscular flexor não apresentou diferença significativa das mesmas variávei. O software desenvolvido em linguagem C /Matlab permitiu melhor visualização, interpretação e análise da performance muscular, oferecendo resultados consistentes para todos os atletas avaliados. Os resultados indicam que os 6 meses de reabilitação não foram suficientes para promover simetria de força entre os lados para o grupo muscular extensor do joelho, indicando um risco de retorno ao esporte nesta fase do tratamento.
Small Structural Differences in Proline-Rich Decapeptides Have Specific Effects on Oxidative Stress-Induced Neurotoxicity and L-Arginine Generation by Arginosuccinate Synthase
(MDPI) Silva, Carlos Alberto; Silva, Brenda Rufino; Silva, Julio Cezar Araujo; Silva, Felipe Assumpção da Cunha e; Kodama, Roberto Tadashi; Silva, Wilmar Dias da; Costa, Maricilia Silva; Portaro, Fernanda Calheta Vieira
ntroduction. The proline-rich decapeptide 10c (Bj-PRO-10c; ENWPHPQIPP) from the Bothrops jararaca snake modulates argininosuccinate synthetase (AsS) activity to stimulate L-arginine metabolite production and neuroprotection in the SH-SY5Y cell line. The relationships between structure, interactions with AsS, and neuroprotection are little known. We evaluated the neuro- protective effects of Bj-PRO-10c and three other PROs (Bn-PRO-10a, Bn-PRO-10c > Bn-PRO-10a-MK > Bn-PRO-10a. The structure of PROs and their correlations with enzyme activity revealed that histidine (H5) and glutamine (Q7) in Bj-PRO-10c potentiated their affinity for AsS. Conclusions. Our investigation provides the first insights into the structure and molecular interactions of PROs with AsS, which could possibly further their neuropharmacological applications.
Molecular Markers for Thyroid Cancer Diagnosis: Insights from MAPK Pathway Gene Expression Analysis
(MDPI) Pupin, Breno; Diniz, Ramon Varella; Costa, Maricilia Silva; Chagas, Maurilio José; Santos, André Bandiera de Oliveira; Canevari, Renata de Azevedo
Background and Objectives: Thyroid cancer is the prevailing endocrine malignancy, with incidence growing over the last decades in the world. The current diagnostic techniques often yield inconclusive results, emphasizing the need for more effective diagnostic ap- proaches. Molecular profiling emerges as a promising avenue for carcinoma differentiation, offering precise insights to guide patient selection for surgical intervention. This study aimed to identify molecular markers in thyroid cancer through the expression analysis of genes within the MAPK pathway, aiming to enhance the sensitivity and specificity of carcinoma diagnosis. Methods: Through a comparative analysis of malignant and benign thyroid samples, we identified 46 genes of the MAPK pathway that exhibited differential expression by PCR array analysis. Results: Validation through RT-qPCR and in silico analysis using TCGA confirmed significant results for CCNA1, CDKN1C, CREB1, FOS, HSPA5, JUN, MAP2K6, and SFN genes identified in our cohort, reinforcing the relevance of these biomarkers. Specifically, noteworthy are our findings regarding the potential diag- nostic value of CCNA1 and SFN genes in papillary thyroid carcinoma, while the reduced expression of CDKN1C, FOS, and JUN genes in follicular carcinoma suggests their value in distinguishing the thyroid pathologies. Conclusions: This study identifies promising diagnostic markers, namely CCNA1, CDKN1C, FOS, JUN, and SFN genes, which have the potential to enhance clinical decision-making in thyroid cancer.
Inhibition of Development and Metabolism of Dual-Species Biofilms of Candida albicans and Candida krusei (Pichia kudriavzevii) by Organoselenium Compounds
(MDPI) Calvi, Gabriela de Souza; Cartaxo, Giulia Nicolle Jácome; Carretoni, Qiuxin Lin; Silva, André Luiz Missio da; Moraes, Denilson Nogueira de; Pradella, José Geraldo da Cruz; Costa, Maricilia Silva
Although Candida albicans is the most frequently identified Candida species in clinical settings, a significant number of infections related to the non-albicans Candida (NAC) species, Candida krusei, has been reported. Both species are able to produce biofilms and have been an important resistance-related factor to antimicrobial resistance. In addition, the microbial relationship is common in the human body, contributing to the formation of polymicrobial biofilms. Considering the great number of reports showing the increase in cases of resistance to the available antifungal drugs, the development of new and effective antifungal agents is critical. The inhibitory effect of Organose- lenium Compounds (OCs) on the development of Candida albicans and Candida krusei was recently demonstrated, supporting the potential of these compounds as efficient antifungal drugs. In addition, OCs were able to reduce the viability and the development of biofilms, a very important step in colo- nization and infection caused by fungi. Thus, the objective of this study was to investigate the effect of the Organoselenium Compounds (p-MeOPhSe)2, (PhSe)2, and (p-Cl-PhSe)2 on the development of dual-species biofilms of Candida albicans and Candida krusei produced using either RPMI-1640 or Sabouraud Dextrose Broth (SDB) media. The development of dual-species biofilms was evaluated by the determination of both metabolic activity, using a metabolic assay based on the reduction of XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt) assay and identification of either Candida albicans and Candida krusei on CHROMagar Candida medium. Biofilm formation using RPMI-1640 was inhibited in 90, 55, and 20% by 30 μM (p-MeOPhSe)2, (PhSe)2, and (p-Cl-PhSe)2, respectively. However, biofilms produced using SDB presented an inhibition of 62, 30 and 15% in the presence of 30 μM (p-MeOPhSe)2, (PhSe)2, and (p-Cl-PhSe)2, respectively. The metabolic activity of 24 h biofilms was inhibited by 35, 30 and 20% by 30 μM (p-MeOPhSe)2, (PhSe)2, and (p-Cl-PhSe)2, respectively, with RPMI-1640; however, 24 h biofilms formed using SDB were not modified by the OCs. In addition, a great reduction in the number of CFUs of Candida albicans (93%) in biofilms produced using RPMI-1640 in the presence of 30 μM (p-MeOPhSe)2 was observed. However, biofilms formed using SDB and treated with 30 μM (p-MeOPhSe)2 presented a reduction of 97 and 69% in the number of CFUs of Candida albicans and Candida krusei, respectively. These results demonstrated that Organoselenium Compounds, mainly (p-MeOPhSe)2, are able to decrease the metabolic activity of dual-species biofilms by reducing both Candida albicans and Candida krusei cell number during biofilm formation using either RPMI-1640 or SDB. Taken together, these results demonstrated the potential of the OCs to inhibit the development of dual-species biofilms of Candida albicans and Candida krusei.
Anti‐inflammatory effect of photobiomodulation in the brain following local peripheral carrageenan‐induced inflammation
(Springer) Silva, Carlos Alberto; Calvi, Gabriela de Souza; Feliciano, Regiane dos Santos; Silva Junior, José Antônio; Costa, Maricilia Silva
Purpose Photobiomodulation (PBM) has been suggested as a potential anti-inflammatory therapy, presenting excellent outcomes for several disorders. Reduction in COX-2 mRNA expression in subplantar and brain tissues by PBM was dem- onstrated, using the classic model of oedema formation and hyperalgesia induced by Carrageenan (Carr). This work inves- tigated the effect of PBM on mRNA expression of key inflammation-related genes (IL-1β, mPGES-1, mPGES-2 and EP) in subplantar and brain tissues obtained from rats receiving Carr. Methods The animals were treated with Carr, treated with PBM after 1 h and sacrificed after 1, 3 and 6 h. The light source used was a diode laser, with output power of 30 mW and a wavelength of 660 nm. The laser beam illuminated an area of 0.785 cm2, resulting in an energy dosage of 7.5 J/cm2, applied for 196 s. IL-1β, mPGES-1, mPGES-2 and EP mRNAs were determined by RT-PCR. Results It was observed a reduction in IL-1β expression as in subplantar tissue as in brain parenchyma in animals treated with PBM. In addition, the expression of both mPGES-1 and mPGES-2 mRNA was decreased after PBM. Conclusion These results suggested that PBM could reduce the production of IL-1β and subsequently decreasing the effects of PGE2. Therefore, the possible mechanism by which PBM alleviates hyperalgesia could involve its ability to decrease the expression of inflammatory markers in the CNS, reducing the production of PGE2 in the spinal cord.
Transcutaneous laser irradiation of blood in simultaneous mode (S-ILIB) for fatigue recovery: a case series
(Universidade do Vale do Paraíba) Soares, Luís Eduardo Silva
A irradiação transcutânea do sangue com laser (ILIB), modalidade sistêmica de terapia de fotobiomodulação (PBMT), consiste na aplicação de laser na artéria radial ou outra artéria, para melhorar as funções sistêmicas e a dinâmica fisiológica do corpo. As estratégias terapêuticas disponíveis para o manejo da fadiga devido ao estresse da vida diária concentram-se principalmente em mudanças no estilo de vida associadas à nutrição e ao exercício. Assim, o presente estudo tem como objetivo relatar uma série de casos em que a terapia ILIB transcutânea, com a técnica modificada e simultânea (S-ILIB), foi utilizada para tratar sintomas de fadiga. Quatro pacientes saudáveis, sem qualquer tipo de comorbidade significativa, relataram fadiga relacionada ao estresse. Foram tratados pelo novo protocolo de PBMT com a técnica S-ILIB modificada. A intensidade ou percepção da fadiga foi avaliada por meio de uma escala visual analógica (EVA). A "técnica simultânea" utilizada no presente estudo consistiu na irradiação do laser em quatro locais do corpo: 1- região da artéria radial do braço direito; 2- região da artéria radial do braço esquerdo; 3- artéria pediosa direita; e 4- artéria pediosa esquerda. Para a PBMT foram utilizados dois aparelhos laser de 660 nm, em modo contato, com potência de 100 mW. Os pacientes relataram uma clara recuperação da sensação energética após a sessão de S-ILIB. O protocolo de PBMT parece ser uma terapia promissora para gerenciar a recuperação energética em rotinas diárias relacionadas ao estresse e fadiga; entretanto, são necessários estudos complementares para estabelecer o protocolo mais seguro e eficiente.
Dentifrícios à base de carvão ativado: análises química e morfológica
(Centro Universitário UninCor) Alvarenga, Marian Pereira; Soares, Luís Eduardo Silva
Este estudo in vitro caracterizou os elementos inorgânicos em dentifrícios à base de carvão vegetal em termos de morfologia e composição. Os dentifrícios comerciais disponíveis no mercado Oral-B Complete® (OBC; controle negativo), o creme dental clareador Colgate Luminous White Expert® (LWE; controle positivo) e os dentifrícios à base de carvão ativado Colgate Natural Extracts Purifying® (CNE), Curaprox Black Is White® (CBW) e Oral-B 3D White Mineral Clean® (OBW) foram avaliados. As análises de microscopia eletrônica de varredura (MEV) e micro fluorescência de raios x por energia dispersiva (μ- EDXRF) foram utilizadas para analisar as frações particuladas dos cremes dentais. A análise MEV das partículas do dentifrício revelou que elas eram maiores e isoladas nos grupos OBC e LWE, eram menores e aglomeradas com formas definidas e tamanhos variados nos grupos CBW e OBW, e apresentavam padrão misto no grupo CNE. Foram observadas diferenças na porcentagem de sílica nos dentifrícios avaliados. Os dentifrícios contendo carvão, com base na sua composição e morfologia das partículas, podem resultar em uma possível perda dentária diferentes em níveis de após a utilização.
Degradação de superfície de uma resina nanoparticulada após exposição a enxaguatórios bucais contendo álcool e agentes clareadores
(Universidade do Vale do Paraíba) Oliveira, Scarlet Ohanna; Soares, Luís Eduardo Silva
Atualmente, existe uma grande busca por estética dental, tanto por parte dos cirurgiões dentistas, quanto dos pacientes. Dentre tantas opções de tratamento, encontram-se disponíveis e de fácil acesso aos pacientes diversos tipos de enxaguatórios bucais. Entretanto, os efeitos destes agentes nas resinas compostas são amplamente discutidos e questionados. Este trabalho tem como objetivo avaliar, através da análise de micro fluorescência de raios x por energia dispersiva (μ-EDXRF) e microscopia eletrônica de varredura (MEV), os efeitos dos enxaguatórios bucais clareadores e contendo álcool, na superfície de uma resina composta de nanopartículas. Foram preparadas 50 amostras de resina composta (Z350, cor EA3) e divididas em 5 grupos: SA - saliva artificial (controle negativo); CP - enxaguatório sem álcool Colgate Plax (controle positivo); L - enxaguatório com álcool Listerine; OW – enxaguatório clareador Oral B e CW - enxaguatório clareador Colgate. As amostras foram imersas em 2ml de enxaguatório e saliva por 24h. Mapeamentos em área da superfície da amostra foram realizados por μ-EDXRF (20 x 20). Comparações dos valores médios da porcentagem em peso de Zircônia (Zr) e Sílica (Si) foram realizadas. Ocorreu aumento da porcentagem em peso de Si e Zr em todos os grupos. O grau de exposição da matriz inorgânica foi maior na ordem CW > OW > L > CP >SA. Os produtos CW e OW resultaram em uma maior exposição de nanoclusters do que os outros grupos. Todos os enxaguatórios causaram alteração na resina composta após utilização simulada de 2 anos.
Os benefícios do uso do enxerto da Membrana Amniótica Humana no âmbito de feridas cirúrgicas, ferimentos e lesões
(Acervo +) Gonçalves, Nicolas Cardoso; Ribeiro, David Pinto; Gouvea, Hilda Cristina Rodrigues; Pedroso, Katia Zeny Assumpção; Silva, Nilson Thiago De Carvalho e; Sant'Anna, Luciana Barros
Realizar uma busca na literatura a respeito dos benefícios associados ao uso do enxerto da membrana amniótica humana (MAH), no âmbito ambulatorial e cirúrgico. Métodos: Trata-se de uma revisão integrativa cuja as bases de pesquisas foram: SciELO, PubMed, Biblioteca Virtual em Saúde e Capes. Foram utilizados estudos in vivo entre 2018-2023. Resultados: Após aplicação dos critérios de inclusão e exclusão, foram identificados 23 artigos. A membrana amniótica humana obteve resultados satisfatórios no tratamento de lesões cirúrgicas, queimaduras, úlceras, lesões na córnea, feridas agudas e crônicas, lesões em nervos, tendões, cartilagem e entre outras. Os estudos relataram que a MAH possui analgesia, ação anti-inflamatória e antimicrobiana, promove a epitelização e a cicatrização, reduz a fibrose, a substituição de curativos e o desconforto em realizar atividades e possui biocompatibilidade. Considerações finais: Dessa forma, esse estudo conseguiu investigar os diversos benefícios relativos ao transplante de MAH para o tratamento de lesões e ferimentos. O uso da MAH é uma possibilidade promissora no âmbito ambulatorial e cirúrgico, tendo em vista que possuem diversos benefícios para a cicatrização e epitelização de uma lesão.
Evaluation of the antimicrobial action of plasma activated water on amniotic membrane
(Springer) Almeida, Felipe Santos de; Doria, Anelise Cristina Osório Cesar; Sant’Anna, Luciana Barros
ntroduction The potential use of the Amniotic Membrane (AM) in different applications has been widely studied and the need to guarantee the sterility of this material is essential for its use. Due to the increase in bacterial resistance, it is important to research new sterilization techniques. Material and methods The aim of this study was to analyze the antimicrobial action of plasma-activated reverse osmosis (RO) water on contaminated amniotic membrane using ATCC® strain of Escherichia coli (25922), Klebsiella pneumoniae (13883), Staphylococcus aureus (6538). The AM was contaminated, after which the PAW was placed in contact with the AM for 90 min. Plasma, gliding arc of argon and compressed air were used to activate the water. Results The results obtained were a cell viability of 11% with the refrigerated PAW and 15% with the ambient PAW for K. pneumoniae, 9% with the refrigerated PAW and 13% with the ambient PAW for S. aureus and 10% with the refrigerated PAW and 14% with the ambient PAW for E. coli, observing a better antimicrobial action of the refrigerated PAW. A greater reduction in CFU was observed when using refrigerated PAW + AM. Conclusion It is concluded that PAWs have a significant antimicrobial action, but not enough to perform AM sterilization.
Analysis of the Protective Potential of the Amniotic Membrane in an In Vitro Experimental Model of Demyelination in Mouse Brain Organotypic Slices
(ACS Publications) Guimarães, Melissa; Calheiro, Gabriela Antonia Tie; Sant'Anna, Luciana Barros
Amniotic membrane (AM) is a biological material recognized for its regenerative, anti-inflammatory, and immunomodulatory properties, constituting a promising approach for the treatment of neurodegenerative diseases, such as demyelinating diseases. Some neurodegenerative diseases, such as Multiple Sclerosis (MS), occur with demyelination, which is a process characterized by the loss of myelin, a structure responsible for the adequate conduction of nerve impulses, compromising neuronal functionality. In this context, this study aimed to investigate the efficacy of AM in protecting nervous tissue against the demyelinating effects of lysophosphatidylcholine (LPC, lysolecithin), using organotypic brain slices from C57BL/6 mice as an in vitro experimental model. Four experimental groups were established: C−H (healthy slices), C- DEM (slices demyelinated with LPC), C-AM (healthy slices with AM), and AM-LPC (slices protected by AM before LPC). The analyses included histological staining (Hematoxylin and Eosin, Luxol Fast Blue), metabolic test with 2,3,5-triphenyltetrazolium chloride (TTC), and Scanning Electron Microscopy (SEM). Results showed that AM preserved myelin and tissue architecture in the challenged slices, while the demyelination group presented microcavitations, structural disorganization, and loss of distinction between white and gray matter. The TTC assay revealed high metabolic activity in the slices protected by AM, in contrast with the low activity in the demyelinated group. SEM analysis reinforced the efficacy of AM, evidencing a preserved organization of the brain parenchyma in slices protected by AM. Thus, the results demonstrate that AM was effective in protecting nervous tissue against the demyelinating effects of lysophosphatidylcholine, preserving myelin, structural organization, and metabolic activity of brain slices, as evidenced by histological, metabolic, and ultrastructural analyses with SEM.
Amniotic membrane modulates MMP9 and MMP12 gene and protein expression in experimental model of the hepatic fibrosis
(Academia Brasileira de Ciências) Alves, Ana Paula da Silva; Teixeira, Roberta Jenniffer Maciel; Silva, Raissa Monteiro da; Canevari, Renata de Azevedo; Sant’Anna, Luciana Barros
Hepatic fibrosis is characterized by excessive deposition of collagen in the hepatic parenchyma, which disturbs the normal architecture and function. We have shown that human amniotic membrane (AM) can be used as a patch on the whole liver surface, resulting in an extremely significant reduction in collagen deposition. The aim of this study was to investigate the effects of AM on the matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 12 (MMP12) genes and proteins expression by real time quantitative PCR and immunohistochemistry, respectively, as well as image analysis on biliary fibrosis induced in rats by the bile duct ligation (BDL).Two weeks after the BDL, an AM fragment was applied onto the liver, and four weeks later, the liver samples were collected. MMP9 and MMP12 genes were significantly over expressed in group treated with AM. The immunoexpression of MMP9 and MMP12 was observed in all groups. However, the quantitative image analysis demonstrated an increase of the area occupied only by MMP12 in the livers of AM-treated rats with respect to BDL rats. These findings suggest that the AM exerts its beneficial effects on biliary fibrosis by increasing the MMP12, which in turn reduces the excessive collagen deposition on liver tissue.
Cost-effective production process of scFv antibody fragments against Shiga toxin 2 via recombinant E. coli
(Elsevier) Guimarães, Marcela; Luz, Daniela; Augusto, Elisabeth de Fátima Pires; Vieira, Lucia; Costa, Maricilia Silva; Piazza, Roxane Maria Fontes; Pradella, José Geraldo da Cruz
Shiga toxin (Stx)-producing Escherichia coli (STEC) and its subgroup enterohemorrhagic E. coli are significant pathogens responsible for diarrhea, which can progress to hemorrhagic colitis and hemolytic uremic syndrome (HUS), the leading cause of acute renal failure in children. Early diagnosis is crucial for effective clinical man- agement, as antibiotic treatment is not recommended for STEC infections. The present study aimed to establish a cost-effective biotechnological platform for cultivating recombinant E. coli to produce scFv antibody fragments against Stx2 for diagnostic applications. The method was first evaluated through shake flask experiments and subsequently scaled up to bench-scale bioreactors operated in both batch and fed-batch modes using defined culture media. Optimal production conditions were achieved by inducing recombinant E. coli pLys at 18 ◦C for 18 h with 0.1 mM IPTG, resulting in a yield of 3.0 to 4.0 mg scFv/g cell biomass. A fed-batch, high-cell-density procedure with E. coli pLysS achieved a maximum production up to 150 mg scFv/L. A preliminary economic assessment demonstrated the production potential at a value of around $250/g scFv. Economic analysis also highlights that the relative cost of capital investment becomes important as production processes intensify. Therefore, technical parameters such as productivity (scFv mass/bioreactor volume * time) and scFv concentration (mass scFv mass/bioreactor volume) should be prioritized to maximize their values. Similarly, optimization of the recombinant E. coli microbial platform should be pursued to increase the Yp/x level.
A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection
(Frontiersin) Razzino, Claudia do Amaral; Sgobbi, Lívia Flório; Cancino-Bernardi, Juliana; Zapata, Angelica Maria Mazuera; Costa, Clara Cardoso; Zucolotto, Valtencir; Vieira, Lucia; Lobo, Anderson Oliveira
Alzheimer’s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of β-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer’s disease is the decreased level of soluble β-amyloid peptide (1–42) (Aβ1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aβ1-42 levels drop below 192 pg mL-1 . In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aβ1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of −200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL-1 , with a detection limit of 7.4 pg mL-1 , exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer’s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.
Tribocorrosion Susceptibility and Cell Viability Study of 316L Stainless Steel and Ti6Al4V Titanium Alloy with and without DLC Coatings
(MDPI) Sene, Ana Claudia; Silva, Michely Glenda Pereira da; Macário, Paulo Fabrício; Vieira, Angela Aparecida; Leite, Priscila Maria Sarmeiro Correa Marciano; Silva, Newton Soares da; Marques, Francisco Das Chagas; Vieira, Lúcia
Stainless steel (SS316L) and titanium alloy (Ti6Al4V) exhibit suitable properties for biomed- ical applications; however, the tribocorrosion of these materials, which is associated with metallosis, is still a significant concern. This work investigates the effectiveness of DLC smoothing coatings applied to the metals to reduce tribocorrosion and improve cell viability. The study was motivated by many reports of metallosis caused by metal debris in the soft tissues of the body. DLC coatings were produced using the plasma-enhanced chemical vapor deposition (PECVD) technique. The cytotoxicity, genotoxicity, and cell viability of metallic samples with and without DLC coatings were analyzed, considering the chemical composition of the coating and metallic components. The results show that the DLC coatings presented suitable interaction properties and no cytotoxicity or genotoxicity when exposed to the cellular environment, compared with the control group (p < 0.0001). They also demonstrated cell viability, low friction representing a reduction of 80%, and hardness 23–26 GPa, making them ideal for use on fixed implants. It is necessary to control the thickness and roughness of the coating to avoid pinholes and increase the corrosion protection of implants. These DLC coatings with low friction coefficients could facilitate the fixation of implantable pins and screws, including Kirschner wires.
Nano-Enabled Colorimetric Assay for the Detection of Paracoccidioides lutzii: Advancing Diagnostics with Nanotechnology
(MDPI) Comparato Filho, Olavo de Osti; Cândido, Marcela Aparecida; Ventura, Aveline; Morais, Flavia Villaça; Raniero, Leandro José
Deforestation is a common occurrence driven by agricultural expansion, urbanization, and infrastructure development. These activities often lead to increased human interaction with ecosys- tems, potentially exposing individuals to Paracoccidioides spores (P. brasiliensis and P. lutzii) found in the soil, resulting in Paracoccidioidomycosis (PCM). This fungal infection is endemic to specific regions in Latin America, such as Brazil, Colombia, Venezuela, and Argentina. Diagnosis typically involves a combination of clinical assessment, imaging techniques, and laboratory examinations. P. lutzii lacks the glycoprotein Gp43, a key antigenic protein utilized in serological tests for PCM diagnosis. In this study, a colorimetric test employing gold nanoparticles (AuNPs) and label-free methodology was employed for P. lutzii detection. The effectiveness of the label-free colorimetric test was assessed using a total of 100 samples. This detection was achieved through the amplification of the gp43 gene and the use of a specific probe (5′CAGGGGTGCG3′) in conjunction with AuNPs. The receiver operating characteristic curve was employed to assess the test, revealing that the method can accurately detect P. lutzii with a sensitivity of 100% and a specificity of 100%. The findings indicate a substantial impact on remote endemic regions attributable to the implementation of cost-effective diagnostic methodologies.

Navegar

Submissões Recentes