Self-assembly and 3D Bioprinting of Neurospheres and Evaluation of Caffeine and Photobiomodulation Effects in an Alzheimer's Disease In Vitro Model

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dc.contributor.authorSalles, Geisa Rodrigues
dc.contributor.authorGranato, Alessandro Eustáquio Campos
dc.contributor.authorViero, Fernanda Tibolla
dc.contributor.authorSoares, Cristina Pacheco
dc.contributor.authorFerreira, Sergio Teixeira
dc.contributor.authorPorcionatto, Marimélia Aparecida
dc.contributor.authorUlrich, Henning
dc.date.accessioned2025-08-26T13:25:35Z
dc.date.available2025-08-26T13:25:35Z
dc.date.issued22025
dc.description.abstractSeveral in vitro models of Alzheimer’s disease (AD) rely on 2D cell culture, and, more recently, 3D cultures represented by free-floating neurospheres have been used as models for the disease. The advantage of 3D over 2D cell culture is that cell-extracellular matrix and cell-cell interactions can be assessed, better representing the molecular and cellular hallmarks of the disease. In the current study, we developed two complementary 3D neurosphere models using SH-SY5Y human neuroblastoma cells to investigate AD pathology and evaluate potential therapies. First, self-assembled neurospheres were exposed to hydrogen peroxide (H2O2) and amyloid-beta oligomers (AβOs), inducing AD-like features such as increased production of reactive oxygen species (ROS), amyloid aggregation, and apoptosis. Treatment with caffeine or photo- biomodulation (PBM) using LED irradiation significantly reduced Aβ1−42 accumulation, ROS generation, and decreased apoptosis markers. Second, 3D bioprinting of SH-SY5Y cells resulted in neurospheres with enhanced cellular organization and differentiation. These findings emphasize the advantages of 3D models for studying neurodegeneration and evaluating therapeutic strategies, bridging the gap between traditional 2D cultures and complex in vitro systems.
dc.description.physical13 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP (2022/08664-4, 2018/12605-8, 2018/2018/0763-4) CNPq (406258/2022- 8, 311026/2022-2, 465656/2014-5) CAPES (33002010017PO)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Federal de São Paulo
dc.identifier.affiliationUniversidade do Estado de São Paulo
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.affiliationUniversidade Federal do Rio de Janeiro
dc.identifier.affiliationInstituto D'Or de Pesquisa e Ensino
dc.identifier.bibliographicCitationSALLES, G. R. et al. Self-assembly and 3D Bioprinting of Neurospheres and Evaluation of Caffeine and Photobiomodulation Effects in an Alzheimer's Disease In Vitro Model. Stem Cell Reviews and Reports, v. 21, n. 4, p. 988-1000, 2025. Disponível em: 10.1007/s12015-025-10850-7.
dc.identifier.doi10.1007/s12015-025-10850-7
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1042
dc.language.isoen_US
dc.publisherSpringer Nature Link
dc.rights.holderStem Cell Reviews and Reports
dc.subject.keywordNeurospheroids
dc.subject.keywordCaffeine
dc.subject.keywordPhotobiomodulation
dc.subject.keywordBioprinting
dc.titleSelf-assembly and 3D Bioprinting of Neurospheres and Evaluation of Caffeine and Photobiomodulation Effects in an Alzheimer's Disease In Vitro Model
dc.typeArtigos de Periódicos

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