Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine

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dc.contributor.authorGarcia, Maíra Terra
dc.contributor.authorWard, Rafael Araújo da Costa
dc.contributor.authorGonçalves, Nathália Maria Ferreira
dc.contributor.authorPedroso, Lara Luise Castro
dc.contributor.authorSilva Neto, José Vieira da
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorJunqueira, Juliana Campos
dc.date.accessioned2025-04-22T13:38:12Z
dc.date.available2025-04-22T13:38:12Z
dc.date.issued22021-11-10
dc.description.abstractPhotodynamic therapy (PDT) mediated by Fotoenticine® (FTC), a new photosensitizer derived from chlorin e-6, has shown in vitro inhibitory activity against the cariogenic bacterium Streptococcus mutans. However, its antimicrobial effects must be investigated on biofilm models that represent the microbial complexity of caries. Thus, we evaluated the efficacy of FTC-mediated PDT on microcosm biofilms of dental caries. Decayed dentin samples were collected from different patients to form in vitro biofilms. Biofilms were treated with FTC associated with LED irradiation and analyzed by counting the colony forming units (log10 CFU) in selective and non-selective culture media. Furthermore, the biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis, respectively. The biofilms from different patients showed variations in microbial composition, being formed by streptococci, lactobacilli and yeasts. Altogether, PDT decreased up to 3.7 log10 CFU of total microorganisms, 2.8 log10 CFU of streptococci, 3.2 log10 CFU of lactobacilli and 3.2 log10 CFU of yeasts, and reached eradication of mutans streptococci. PDT was also capable of disaggregating the biofilms and reducing acid concentration in 1.1 to 1.9 mmol lactate/L. It was concluded that FTC was effective in PDT against the heterogeneous biofilms of dental caries.
dc.description.physical15 p.
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriCNPq: 306330/2018-0, 408369/2018-3; CAPES: scholarship for MTG.; FAPESP: 2020/08345-0, 2020/06909-4.
dc.format.mimetypepdf
dc.identifier.affiliationDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP
dc.identifier.affiliationDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP
dc.identifier.affiliationDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP
dc.identifier.affiliationDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP
dc.identifier.affiliationAssociate Laboratory of Sensors and Materials/LABAS, National Institute for Space Research
dc.identifier.affiliationPhotobiology Applied to Health, Research and Development Institute IP&D, University of Vale do Paraiba/UNIVAP
dc.identifier.affiliationDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology/ICT, São Paulo State University/UNESP
dc.identifier.bibliographicCitationGARCIA, M. T. et al. Susceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine. Pharmaceutics, [s. l.], v. 13, n. 11, p. 1907, 2021. Disponível em: https://www.mdpi.com/1999-4923/13/11/1907.
dc.identifier.doihttps://doi.org/10.3390/pharmaceutics13111907
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/862
dc.language.isoen
dc.publisherMDPI
dc.rights.holderMaíra Terra Garcia
dc.rights.holderRafael Araújo da Costa Ward
dc.rights.holderNathália Maria Ferreira Gonçalves
dc.rights.holderLara Luise Castro Pedroso
dc.rights.holderJosé Vieira da Silva Neto
dc.rights.holderJuliana Ferreira Strixino
dc.rights.holderJuliana Campos Junqueira
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordDental caries
dc.subject.keywordOral biofilms
dc.subject.keywordFotoenticine
dc.subject.keywordChlorine
dc.subject.keywordMethylene blue
dc.titleSusceptibility of Dental Caries Microcosm Biofilms to Photodynamic Therapy Mediated by Fotoenticine
dc.typeArtigos de Periódicos

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