Assessment of the Effect of Antimicrobial Photodynamic Therapy on Multidrug- Resistant Acinetobacter baumannii Strain Biofilm - an in Vitro Study

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dc.contributor.authorBrambilla, Isabelle de Paula Ribeiro
dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.eventRibeirão Preto
dc.date.accessioned2026-02-26T14:28:47Z
dc.date.available2026-02-26T14:28:47Z
dc.date.issued32025
dc.description.abstractAcinetobacter baumannii is an opportunistic bacterium associated with numerous cases of hospital-acquired infections and possesses, among other characteristics that make it virulent, resistance to various antibiotics. The increasing resistance of microorganisms has led to the search for alternative methods, such as Antimicrobial Photodynamic Therapy (aPDT), inducing bacterial cell death. This study aimed to evaluate the antimicrobial action of PDT with curcumin on A. baumannii biofilm in vitro. A. baumannii biofilm was treated with curcumin for 20 min and then irradiated with a 450 nm LED (Light Emitting Diode) at 93.9 mW/cm2, with a fluence of 42.72 J/cm2. Subsequently, tests were conducted using Confocal Microscopy with the Resazurin dye, Colony-Forming Units (CFU) counting, and Scanning Electron Microscopy (SEM) imaging. Cell viability tests were also conducted using the L929 fibroblast cell line. The results show that although PDT with curcumin did not reduce bacterial growth, it caused alterations in cell metabolism. Additionally, confocal microscopy showed that curcumin was internalized by A. baumannii at the concentrations used. SEM demonstrated structural changes, although no bacterial reduction in the biofilm was observed. The parameters used in biofilm treatment were cytotoxic to L929 cells. This study showed that PDT with curcumin induces fragility in A. baumannii cells, suggesting that a possible combination with antibiotics may lead to the elimination of bacterial infection.
dc.description.physical10 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFAPESP: 2016/12211-4, 2022/10251-0, 2013/07276-1 FINEP: 01.12.0275.00
dc.format.mimetypePDF
dc.identifier.bibliographicCitationBRAMBILLA, I. P. R. et al. Assessment of the Effect of Antimicrobial Photodynamic Therapy on Multidrug- Resistant Acinetobacter baumannii Strain Biofilm - an in Vitro Study. In: Brazilian Congress on Biomedical Engineering, 29., 2025. Proceedings..., Ribeirão Preto: Springer Nature, 2025. Disponível em: 10.1007/978-3-031-94921-0_29.
dc.identifier.doi10.1007/978-3-031-94921-0_29
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1157
dc.language.isoen_US
dc.rights.holderIFMBE Proceedings
dc.subject.keywordAntimicrobial therapy
dc.subject.keywordBacterial transformation
dc.subject.keywordBiofilms
dc.subject.keywordDental Biofilms
dc.subject.keywordPhotocatalysis
dc.subject.keywordAntibacterial drug resistance
dc.titleAssessment of the Effect of Antimicrobial Photodynamic Therapy on Multidrug- Resistant Acinetobacter baumannii Strain Biofilm - an in Vitro Study
dc.typeAnais de Congresso
dc.type.eventCongresso

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