PCR analysis of the effect of photodynamic therapy on breast tumors

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dc.contributor.authorFerreira, Isabelle
dc.contributor.authorSilva, Glenda Nicioli da
dc.contributor.authorFerreira-Strixino, Juliana
dc.contributor.authorGrecco, Clovis
dc.contributor.authorBagnato, Vanderlei Salvador
dc.contributor.authorSalvadori, Daisy Maria Favero
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorRocha, Noeme Sousa
dc.date.accessioned2025-04-22T13:42:33Z
dc.date.available2025-04-22T13:42:33Z
dc.date.issued22021-09-26
dc.description.abstractPhotodynamic therapy (PDT) is a promising therapeutic modality for treating cancer, including breast tumors. The oxidative damage caused by PDT culminates in cell death, induction of immune response, and the resulting destruction of the tumor. This study aimed to evaluate the gene expression profiling of genes BCL-2, BAX, and HER-2 and their proteins after PDT, associating it with the necrosis caused by this therapy under different fluences. Twenty-eight female rats received a single dose of 7,12-dimethylbenz (a) anthracene (DMBA - 80mg/kg), by gavage, for breast tumor induction. After the tumors grew, the animals were divided into four groups: G1 - control group – untreated breast tumor – and G2, G3, and G4 groups treated with PDT using Photogem@ as photosensitizer and interstitial irradiation, with fluences of 50J/cm, 100J/cm, and 150J/cm, respectively. Samples of tumors were harvested for histological examination by RT-qPCR. The RT-qPCR showed that the gene expression profiling of BCL-2, BAX, and HER-2 was not altered after PDT. Hemorrhagic necrosis and qualitatively greater vascular and cellular damage were observed and correlated positively with the fluence. PDT does not seem to induce the modulation of genes related to apoptosis. The results indicate that the type of cell death stimulated by PDT in breast tumor is necrosis.
dc.description.physical10 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP: Processes No. 09/51826-0 and No. 11/00731-0
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Federal de Ouro Preto
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationCentro de Estudos e Formação Avançada
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.bibliographicCitationFERREIRA, I.; SILVA, G. N. da .; FERREIRA-STRIXINO, J.; GRECCO, C. .; BAGNATO, V. S. .; SALVADORI, D. M. F. .; PINTO, J. G. .; ROCHA, N. S. PCR analysis of the effect of photodynamic therapy on breast tumors . Research, Society and Development, [S. l.], v. 10, n. 12, p. e459101220468, 2021. DOI: 10.33448/rsd-v10i12.20468. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20468. Acesso em: 8 apr. 2025.
dc.identifier.doihttps://doi.org/10.33448/rsd-v10i12.20468
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/863
dc.language.isoen
dc.publisherCDRR Editors
dc.rights.holderIsabelle Ferreira
dc.rights.holderGlenda Nicioli da Silva
dc.rights.holderJuliana Ferreira-Strixino
dc.rights.holderClovis Grecco
dc.rights.holderVanderlei Salvador Bagnato
dc.rights.holderDaisy Maria Favero Salvadori
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderNoeme Sousa Rocha
dc.subject.keywordCancer
dc.subject.keywordGene expression
dc.subject.keywordPhotodynamic therapy
dc.titlePCR analysis of the effect of photodynamic therapy on breast tumors
dc.title.alternativeAnálise por PCR do efeito da terapia fotodinâmica em tumores mamários
dc.typeArtigos de Periódicos

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