Effects of antimicrobial photodynamic therapy with photodithazine® on methicillin-resistant Staphylococcus aureus (MRSA): Studies in biofilms and experimental model with Galleria mellonella

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dc.contributor.authorSouza, Beatriz Müller Nunes
dc.contributor.authorMiñán, Alejandro Guillermo
dc.contributor.authorBrambilla, Isabelle Ribeiro
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorGarcia, Maíra Terra
dc.contributor.authorJunqueira, Juliana Campos
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-02-12T18:45:38Z
dc.date.available2025-02-11T19:01:46Z
dc.date.available2025-02-12T18:10:34Z
dc.date.available2025-02-12T18:45:38Z
dc.date.issued22024-03
dc.description.abstractStaphylococcus aureus infections are a severe health problem due to the high mortality rate. Conventional treatment of these infections is via the administration of antibiotics. However, its indiscriminate use can select resistant microorganisms. Thus, it is necessary to develop alternatives for antibiotic therapy. Antimicrobial Photodynamic Therapy (aPDT), a therapeutic method that associates a photosensitizer (PS), a light source with adequate wavelength to the PS, interacts with molecular oxygen generating reactive oxygen species responsible for cell inactivation, is a viable alternative. This work aimed to analyze, in vitro and in vivo, the action of aPDT with PS Photodithazine® (PDZ) on the methicillin-resistant S. aureus (MRSA) strain. In the in vitro method, the S. aureus biofilm was incubated with PDZ at 50 and 75 μg.mL−1 for 15 min, adopting the light dose of 25, 50, and 100 J/cm2. In addition, PS interaction, formation of reactive oxygen species (ROS), bacterial metabolism, adhesion, bacterial viability, and biofilm structure were evaluated by scanning electron microscopy. Subsequently, the strain was inoculated into models of Galleria mellonella, and the survival curve, health scale, blood cell analysis, and CFU/mL of S. aureus in the hemolymph were analyzed after aPDT. In the in vitro results, bacterial reduction was observed in the different PDZ concentrations, highlighting the parameters of 75 μg.mL−1 of PDZ and 100 J/cm2. As for in vivo results, aPDT increased survival and stimulated the immune system of G. mellonella infected by S. aureus. aPDT proved effective in both models, demonstrating its potential as an alternative therapy in treating MRSA bacterial infections.
dc.description.physical12 f.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFAPESP 2016/12211-4 CAPES Financie Code 001 CNPq: 350140/2022-7 FINEP 01.13.0275/00 CEPOF (2013/07276–1)
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationNational University of La Plata
dc.identifier.affiliationUniversidade Federal de Sao Paulo
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade Estadual Paulista
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationSOUZA, B. M. N. et al. Effects of antimicrobial photodynamic therapy with photodithazine® on methicillin-resistant Staphylococcus aureus (MRSA): Studies in biofilms and experimental model with Galleria mellonella. Journal of Photochemistry and Photobiology B: Biology, [S. l.], v. 252, p. 112860, mar. 2024. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1011134424000204.
dc.identifier.doi10.1016/j.jphotobiol.2024.112860
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/566.3
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderElsevier B.V.
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordPhotodithazine®
dc.subject.keywordGram-positive bacteria
dc.subject.keywordMethicillin-resistant Staphylococcus aureus
dc.subject.keywordChlorine
dc.subject.keywordGalleria mellonella
dc.titleEffects of antimicrobial photodynamic therapy with photodithazine® on methicillin-resistant Staphylococcus aureus (MRSA): Studies in biofilms and experimental model with Galleria mellonella
dc.typeArtigos de Periódicos

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