Molecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes

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dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira, Isabelle
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorCanevari, Renata de Azevedo
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-01-31T16:26:16Z
dc.date.available2025-01-31T16:26:16Z
dc.date.issued22024-05
dc.description.abstractLeishmaniasis is a neglected disease mainly affecting low-income populations. Conventional treatment involves several side effects, is expensive, and, in addition, protozoa can develop resistance. Photodynamic therapy (PDT) is a promising alternative in treating the disease. PDT involves applying light at a specific wavelength to activate a photosensitive compound (photosensitizer, PS), to produce reactive oxygen species (ROS). Curcumin and its photochemical characteristics make it a good candidate for photodynamic therapy. Studies evaluating gene expression can help to understand the molecular events involved in the cell death caused by PDT. In the present study, RNA was extracted from promastigotes from the control and treated groups after applying PDT. RT-qPCR was performed to verify the expression of the putative ATPase beta subunit (ATPS), ATP synthase subunit A (F0F1), argininosuccinate synthase 1 (ASS), ATP-binding cassette subfamily G member 2 (ABCG2), glycoprotein 63 (GP63), superoxide dismutase (FeSODA), and glucose-6-phosphate dehydrogenase (G6PDH) genes (QR). The results suggest that PDT altered the expression of genes that participate in oxidative stress and cell death pathways, such as ATPS, FeSODA, and G6PD. The ATP-F0F1, ASS, and GP63 genes did not have their expression altered. However, it is essential to highlight that other genes may be involved in the molecular mechanisms of oxidative stress and, consequently, in the death of parasites.
dc.description.physical4 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP 2016/12211–4.
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationMARCOLINO, L. M. C. et al. Molecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes. Parasitology Research, [s. l.], v. 123, n. 2, p. 146, 2024.
dc.identifier.doi10.1007/s00436-024-08155-8
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/539
dc.language.isoen_US
dc.publisherParasitology Research, Springer-Verlag GmbH Germany
dc.rights.holderLuciana Maria Cortez Marcolino
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderIsabelle Ferreira
dc.rights.holderBruno Henrique Godoi
dc.rights.holderRenata de Azevedo Canevari
dc.rights.holderJuliana Ferreira‑Strixino
dc.subject.keywordLeishmania
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordCurcumin
dc.subject.keywordGene expression
dc.subject.keywordPDT
dc.titleMolecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes
dc.typeArtigos de Periódicos

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