Analysis of the biomolecular profile by Fourier transform vibrational spectroscopy (FT-IR) in Acinetobacter baumannii after application of photodynamic therapy with curcumin "in vitro "

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dc.contributor.authorMarques, Camila Monteiro Santos
dc.contributor.authorLyra, Lucas Ferreira
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorSakane, Kumiko Koibuchi
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-08-21T18:20:03Z
dc.date.available2025-08-21T18:20:03Z
dc.date.issued22025
dc.description.abstractAcinetobacter baumannii stands out for its antimicrobial resistance and high capacity to cause hospital infections, posing a severe threat to global public health. Thus, there is an urgent need for new therapeutic strategies. This work applied photodynamic therapy (PDT) with curcumin to Acinetobacter baumannii, and bacterial cell viability was assessed. Fourier transform infrared spectroscopy (FT-IR) was used to study changes in biomolecules after PDT application, comparing them with control groups that were only irradiated and only with curcumin photosensitizer. According to the results, there was an increase in the total amount of lipids (~0.3 %), total proteins (~0.6 %), and nuclear material (~8.6 %), with a decrease in carbohydrates (~3.0 %) after the appli- cation of PDT, compared to the control group. In the secondary structure of the proteins, a reduction in α-helix (~3.0 %), disordered structure (~7.0 %), and turns (~5.0 %) was observed, with an increase in β-sheets (~8.8 %). The analysis of biomolecular changes by FT-IR after the application of PDT can contribute to understanding the mechanisms of action of cell death, enabling therapeutic strategies to improve diagnosis and clinical treatment.
dc.description.physical12 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP (2022/10251-0) CAPES (Finance Code 001)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationMARQUES, C. M. S. et al. Analysis of the biomolecular profile by Fourier transform vibrational spectroscopy (FT-IR) in Acinetobacter baumannii after application of photodynamic therapy with curcumin “in vitro ”. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 330, p. 1-12, 2025. Disponível em: 10.1016/j.saa.2025.125740.
dc.identifier.doi10.1016/j.saa.2025.125740
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1030
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
dc.subject.keywordESKAPE
dc.subject.keywordAcinetobacter baumannii
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordCurcumin
dc.subject.keywordFourier Transform Infrared Spectroscopy
dc.subject.keywordSecondary structures of proteins
dc.subject.keywordBiomolecules
dc.titleAnalysis of the biomolecular profile by Fourier transform vibrational spectroscopy (FT-IR) in Acinetobacter baumannii after application of photodynamic therapy with curcumin "in vitro "
dc.typeArtigos de Periódicos

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