Photodynamic Activity of Photogem® in Leishmania Promastigotes and Infected Macrophage

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dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-06-13T13:32:04Z
dc.date.available2025-06-13T13:32:04Z
dc.date.issued22021
dc.description.abstractObjectives: This study aimed to evaluate the effect of photodynamic therapy (PDT) with Photogem⃝R in promastigotes of Leishmania braziliensis and Leishmania major, and in infected macrophages. Materials & methods: The following parameters were analyzed: Photogem⃝R internalization, mitochondrial activ- ity, viability, tubulin marking and morphological alterations in promastigotes and viability in infected macrophages. Results: Photogem⃝R accumulated in the cytosol and adhered to the flagellum. Changes were observed in the mitochondrial activity in groups maintained in the dark, with no viability alteration. After PDT, viability decreased up to 80%, and morphology was affected. Conclusion: The results point out that PDT with Photogem⃝R can reduce parasite and macrophage viability. Lay abstract: Cutaneous Leishmaniasis is a disease that can cause deforming lesions, the treatment of which is highly toxic. It is considered a neglected disease and little progress has been made in the treatment of this disease. Photodynamic therapy can be an alternative treatment which is less costly, and involves local treatment of the lesion, thereby reducing side effects for the patient. The present study aims to test the photodynamic therapy with Photogem, a photo sensitive drug, and to verify if the parasites can be affected by this therapy, aiming to apply this therapy in lesions in patients in the near future.
dc.description.physical13 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriFAPESP (2013/12284-3, 2016 / 12211-4, 2013/07276-1) FINEP (no 01.13.0275.00) CAPES (Financing Code 001)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationPINTO, J. G.; MARCOLINO, L. M. C.; FERREIRA-STRIXINO, J. Photodynamic activity of Photogem in Leishmania promastigotes and infected macrophages. Future Microbiology, v. 16, n. 2, p. 95-1-6, 2021. Disponível em:
dc.identifier.doi10.2217/fmb-2020-0019
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1002
dc.language.isoen_US
dc.publisherTaylor & Francis
dc.rights.holderFuture Microbiology
dc.subject.keywordLeishmania braziliensis
dc.subject.keywordLeishmania major
dc.subject.keywordMacrophages
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordPhotogem
dc.titlePhotodynamic Activity of Photogem® in Leishmania Promastigotes and Infected Macrophage
dc.typeArtigos de Periódicos

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