Action of Photodynamic Therapy at Low Fluence in 9 L/lacZ Cells after Interaction with Chlorins

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dc.contributor.authorVitorio, Gabrielle dos Santos
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorFerreira, Isabelle
dc.contributor.authorPacheco Soares, Cristina
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2025-03-12T16:59:13Z
dc.date.available2025-03-12T16:59:13Z
dc.date.issued22023-02-02
dc.description.abstractGliosarcoma (GS) is a primary malignant neoplasm of the central nervous system, treated with an unfavorable prognosis with surgery, radiotherapy, and chemotherapy. The treatment for GS consists of surgical resection, almost always accompanied by radiotherapy and/or chemotherapy, given the invasive behavior of the tumor. Photodynamic Therapy (PDT) is studied as an alternative method that combines light, a photosensitizer (PS), and molecular oxygen. This study aimed to compare the effects of PDT using the photosensitizers Fotoenticine (FTC) and Photodithazine (PDZ) at low concentrations and fluences. For this study, 9 L/lacZ cells, concentrations of 1.55 µg mL−1 , 12.5 µg mL−1 , and 50 µg mL−1 of chlorins and fluences of 1, 5, and 10 J/cm2 were used. A test was also carried out with Trypan Blue in L929 cells at the mentioned concentrations at 5 J/cm2 . Both chlorins were internalized in the cytoplasm, with a significant reduction in viability (>95%) in almost all groups and altered cell adhesion and morphology after PDT. HSP70 expression decreased in both PS, while HSP27 increased only in PDT with FTC, and although there was a change in cell adhesion in the 9 L/LacZ lineage it was not observed in the L929 fibroblast lineage. Both chlorins were effective, highlighting the concentration of 50 µg mL−1 at the fluence of 5 J/cm2 ; according to the present study, the PDZ showed better results.
dc.description.physical16 f.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFAPESP grant number 2016/12211‑4 FINEP agreement 01.13.0275.00 CAPES Financing Code 001
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationVITORIO, G. S. et al. Action of Photodynamic Therapy at Low Fluence in 9 L/lacZ Cells after Interaction with Chlorins. Photochem, [S. l.], v. 3, n. 1, p. 82–97, 2 fev. 2023. Disponível em: https://www.mdpi.com/2673-7256/3/1/6.
dc.identifier.doi10.3390/photochem3010006
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/684
dc.language.isoen_US
dc.publisherMDPI
dc.rights.holderMDPI
dc.rights.holderGabrielle dos Santos Vitorio
dc.rights.holderBruno Henrique Godoi
dc.rights.holderJuliana Guerra Pinto
dc.rights.holderIsabelle Ferreira
dc.rights.holderCristina Pacheco Soares
dc.rights.holderJuliana Ferreira‑Strixino
dc.subject.keywordGliosarcoma
dc.subject.keywordBrain cancer
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordPhotodithazine
dc.subject.keywordFotoenticine
dc.subject.keywordHeat shock protein
dc.titleAction of Photodynamic Therapy at Low Fluence in 9 L/lacZ Cells after Interaction with Chlorins
dc.typeArtigos de Periódicos

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