Juglans regia and Pfaffia paniculata extracts: implications for periodontal disease treatment and correlation with Alzheimer's risk

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dc.contributor.authorMiranda, Diego Garcia
dc.contributor.authorCarrouel, Florence
dc.contributor.authorAttik, Nina
dc.contributor.authorAraujo, Gabriela Ferraz
dc.contributor.authorLopes, Nicole Fernanda dos Santos
dc.contributor.authorMarcucci, Maria Cristina
dc.contributor.authorRodrigues, Flavia Pires
dc.contributor.authorCaires, Giovanna Arruda
dc.contributor.authorVigerelli, Hugo
dc.contributor.authorGodoi, Bruno Henrique
dc.contributor.authorSoares, Cristina Pacheco
dc.contributor.authorRamos, Lucas de Paula
dc.date.accessioned2025-08-25T17:24:18Z
dc.date.available2025-08-25T17:24:18Z
dc.date.issued22025
dc.description.abstractPeriodontal disease (PD) is a significant global health concern, affecting approximately 19% of the world’s population. It is one of the most prevalent diseases today, causing substantial socio-economic impacts and diminished quality of life. Recent research has also revealed a potential link between PD and Alzheimer’s disease. This study investigated the antimicrobial effects of Juglans regia and Pfaffia paniculata extracts against P. endodontalis and P. gingivalis, bacteria that cause PD and are related to Alzheimer’s risk. The study also assessed the impact of these extracts on macrophage metabolic activity, pro- and anti-inflammatory cytokine expression, and genotoxicity. The phytochemical analysis of the extract was carried out first. Antimicrobial activity was performed using the M11-A7 protocol (CLSI) for planktonic cultures on monotypic biofilms matured for 168 hours in anaerobiosis. Cell viability analysis was carried out using MTT on mouse macrophages (RAW 264-7), as well as genotoxicity assessment using micronuclei. The anti-inflammatory activity was evaluated using ELISA method, checking the cytokines IL-6, IL-1B, TNF-alpha, IL-17 and IL-10. Phytochemical analysis revealed the presence of Miquelianin, Regiolone and Gallic Acid in J. regia extract. For the P. paniculata extract, we identified the glycosides Pfaffoside C, Pfaffoside A, 3-O-β-D-glycopyranosyl-oleanolic acid and Beta-ecdysone. Antimicrobial activity revealed a MBC of 1.73 for the extract of J. regia and 0.48 for P. paniculata against P. endodontalis and P. gingivalis. All biofilms were reduced by more than 89% after treatment with the extracts for 5 min. Cytotoxicity evaluations revealed that cell viability remained above 50% at concentrations up to 0.216 mg/ml for J. regia and 0.015 mg/ml for P. paniculata. Neither extract exhibited genotoxicity. Furthermore, both demonstrated anti-inflammatory activity by promoting the production of the cytokine IL-10. In conclusion, the antimicrobial and anti-inflammatory activities of J. regia and P. paniculata extracts suggest their potential as treatments for oral dysbiosis, which may contribute to a reduced risk of neurodegenerative diseases.
dc.description.physical13 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.uriCAPES (88887.891487/2023-00 and 88887.829787/2023-00)
dc.format.mimetypePDF
dc.identifier.affiliationUniversity of Lyon
dc.identifier.affiliationUniversidade do Estado de São Paulo
dc.identifier.affiliationUniversity of Leeds
dc.identifier.affiliationInstituto Butantan
dc.identifier.affiliationUniversidade Federal de Alfenas
dc.identifier.bibliographicCitationMIRANDA, D. G. et al. Juglans regia and Pfaffia paniculata extracts: implications for periodontal disease treatment and correlation with Alzheimer's risk. Frontiers in Cellular and Infection Microbiology, v. 15, p. 1-13, 2025. Disponível em: 10.3389/fcimb.2025.1585438.
dc.identifier.doi10.3389/fcimb.2025.1585438
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1037
dc.language.isoen_US
dc.publisherFrontiers
dc.rights.holderFrontiers in Cellular and Infection Microbiology
dc.subject.keywordPorphyromonas endodontalis
dc.subject.keywordPorphyromonas gingivalis
dc.subject.keywordNeurodegenerative disease
dc.subject.keywordHerbal medicine
dc.subject.keywordGram-negative anaerobes
dc.subject.keywordAntimicrobial agents
dc.titleJuglans regia and Pfaffia paniculata extracts: implications for periodontal disease treatment and correlation with Alzheimer's risk
dc.typeArtigos de Periódicos

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