Curcumin-loaded bovine serum albumin (BSA) nanoparticles: photoreactivity and photodynamic action against Acinetobacter baumannii and Staphylococcus aureus

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dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorLaneri, Francesca
dc.contributor.authorBrambilla, Isabelle de Paula Ribeiro
dc.contributor.authorAzevedo, Maria Clara Barbosa Silva de
dc.contributor.authorAmbrósio, Jéssica Aparecida Ribeiro
dc.contributor.authorSimioni, Andreza Ribeiro
dc.contributor.authorFraix, Aurore
dc.contributor.authorSortino, Salvatore
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2026-01-08T14:54:26Z
dc.date.available2026-01-08T14:54:26Z
dc.date.issued22024
dc.description.abstractCurcumin (CUR) is a naturally occurring pigment, poorly soluble in water and an object of intense interest due to its multifaceted therapeutic and phototherapeutic activity. In this contribution, bovine serum albumin nanoparticles (BSA-NPs) with oppositely charged surfaces have been used as suitable nanocarriers both to overcome the poor water solubility of CUR and to encourage its interaction with Gram-positive and Gram-negative bacteria. The different surface charges of the BSA-NPs do not affect either the spectroscopic or the photochemical behaviour of the encapsulated CUR which in all cases is almost exclusively present in a diketo form rather than the enolic one. This strictly dictates the response of CUR to blue light excitation which, under these conditions, exhibits the usual reactivity of carbonyl compounds toward molecules that behave as hydrogen donors such as the BSA-NP components. Steady-state and time-resolved photochemical experiments show that the encapsulated CUR under- goes photodecomposition with rate basically independent of the presence of oxygen. The photo- decomposition seems to be mediated mainly by an intermolecular H-abstraction from the components of the nanocarrier by the lowest excited triplet state of CUR with the formation of the corresponding ketyl radical occurring in less than 0.5 ms. This radical is oxidized by molecular oxygen, likely leading to peroxyl and hydroperoxyl radical species, probably responsible for the photodynamic action. Accordingly, with the short triplet lifetime, no singlet oxygen photogeneration is observed. Concentrations of 0.2, 0.3 and 0.4 mM were selected for in vitro tests. Internalization of both formulations was observed in strains of A. baumannii and S. aureus, after 15 minutes of interaction. After irradiation, a reduction in the viability of bacteria was observed, at the different concentrations tested, with concentrations of 0.4 and 0.3 mM being the most efficient.
dc.description.physical11 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriFapesp Grant number 2022/10251-0 FINEP 01.13.0275/00
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversity of Catania
dc.identifier.bibliographicCitationPINTO, J. G. et al. Curcumin-loaded bovine serum albumin (BSA) nanoparticles: photoreactivity and photodynamic action against Acinetobacter baumannii and Staphylococcus aureus. New Journal of Chemistry, v. 48, n. 31, p. 13783-13793, 2024. Disponível em: https://xlink.rsc.org/?DOI=D4NJ01576B.
dc.identifier.doi10.1039/d4nj01576b
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1066
dc.language.isoen_US
dc.publisherRoyal Society of Chemistry
dc.rights.holderNew Journal of Chemistry
dc.subject.keywordPhotodynamic therapy
dc.subject.keywordCurcumin
dc.subject.keywordNanoparticles
dc.titleCurcumin-loaded bovine serum albumin (BSA) nanoparticles: photoreactivity and photodynamic action against Acinetobacter baumannii and Staphylococcus aureus
dc.typeArtigos de Periódicos

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