Biochemical characterization of the strict anaerobic Gram-negative bacteria Tannerella forsythia by infrared micro-spectroscopy

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dc.contributor.authorAbreu, Geraldo Magno Alves de
dc.contributor.authorFontoura, Inglid
dc.contributor.authorCastilho, Maiara Lima
dc.contributor.authorSibelino, Sônia Khouri
dc.contributor.authorSoto, Claudio Alberto Téllez
dc.contributor.authorMartin, Aírton Abrahão
dc.date.accessioned2025-08-21T19:34:51Z
dc.date.available2025-08-21T19:34:51Z
dc.date.issued22025
dc.description.abstractTannerella forsythia is a fastidious S-layer Gram-negative, strict anaerobic, and highly pleomorphic bacterium associated with periodontal inflammatory processes, bone loss, cardiovascular mortality, systemic sequelae, sepsis, and preterm birth. One of the purposes of this study was to characterize, for the first time, Tannerella forsythia (ATCC 43037) using Fourier transform infrared micro-spectroscopy. The analysis aimed to establish a suitable methodology for obtaining reproducible spectra for the characterization of this microorganism, as well as to assign the vibrational bands using techniques such Fourier self-deconvolution analysis (FSD) and second derivative spectral followed by Gaussian band fitting. The study was carried out in triplicate, yielding a total of 25 spectra per culture and 75 spectra across replicates. This methodology can be applied to the biochemical identification and characterization of Tannerella forsythia.
dc.description.physical8 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriCAPES (88887.968153/ 2024-00) FAPESP (09/12082-6 and 2011/22496-2)
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade do Estado de São Paulo
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.bibliographicCitationABREU, G. M. A. et al. Biochemical characterization of the strict anaerobic Gram-negative bacteria Tannerella forsythia by infrared micro-spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 340, p. 1-8, 2025. Disponível em: 10.1016/j.saa.2025.126317.
dc.identifier.doi10.1016/j.saa.2025.126317
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1031
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
dc.subject.keywordTannerella forsythia
dc.subject.keywordFT-IR
dc.subject.keywordGaussian fittings
dc.subject.keywordBiochemical characterization
dc.titleBiochemical characterization of the strict anaerobic Gram-negative bacteria Tannerella forsythia by infrared micro-spectroscopy
dc.typeArtigos de Periódicos

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