Photodynamic treatment in glioma: Metabolic and structural evaluation after therapy

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dc.contributor.authorTeixeira, Marina Gabriela
dc.contributor.authorMarcolino, Luciana Maria Cortez
dc.contributor.authorPinto, Juliana Guerra
dc.contributor.authorAlmeida, Rainara Moreno Sanches de
dc.contributor.authorFerreira, Isabelle
dc.contributor.authorFerreira-Strixino, Juliana
dc.date.accessioned2026-01-15T16:32:58Z
dc.date.available2026-01-15T16:32:58Z
dc.date.issued22025
dc.description.abstractGliomas are malignant tumors of the central nervous system, and one severe variant is called gliosarcoma. Photodynamic therapy (PDT) is a technique that stands out in the oncology area for minimizing side effects for the patient, triggering cell death at the site of irradiation, and can be used concomitantly with conventional treatments. This study aimed to evaluate the interaction of chlorine e6 with the cytoskeleton and mitochondria, as well as morphological changes and the death mechanism trig- gered after PDT. Chlorin e6 was used at concentrations of 200, 12.5, and 6.25 μg/mL, and cytoskeletal changes were analyzed by alpha-tubulin staining and mitochondrial membrane potential (MMP) analysis by JC-1 and Rhodamine 123 in flow cytom- etry. Surface features were examined using scanning electron microscopy, and the type of cell death mechanism was determined by flow cytometry with annexin and propidium iodide. Changes in the cytoskeleton were observed after PDT. Cytometry showed that cell death occurred predominantly via the apoptosis pathway, followed by the necrosis pathway. Chlorin e6 associated with PDT causes damage to gliosar- coma cells, regardless of concentration, showing cytoskeletal disruption, a decrease in MMP, and the percentage of cell death varies according to the concentration of PS.
dc.description.physical12 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP: 2016/12211-4, 2018/15302- 6, 2013/07276-1 FINEP: 01.13.0275.00, 01.18.0053.00 CNPq: 152661/2022-0
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationTEIXEIRA, M. G. et al. Photodynamic treatment in glioma: Metabolic and structural evaluation after therapy. Photochemistry and Photobiology, v. 101, n. 6, p. 1380-1391, 2025. Disponível em: https://onlinelibrary.wiley.com/doi/10.1111/php.70032.
dc.identifier.doi10.1111/php.70032
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1093
dc.language.isoen_US
dc.publisherWiley
dc.rights.holderPhotochemistry and Photobiology
dc.subject.keywordChlorine
dc.subject.keywordCytometry
dc.subject.keywordGliosarcoma
dc.subject.keywordPhotodynamic therapy
dc.titlePhotodynamic treatment in glioma: Metabolic and structural evaluation after therapy
dc.typeArtigos de Periódicos

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