A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection

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dc.contributor.authorRazzino, Claudia do Amaral
dc.contributor.authorSgobbi, Lívia Flório
dc.contributor.authorCancino-Bernardi, Juliana
dc.contributor.authorZapata, Angelica Maria Mazuera
dc.contributor.authorCosta, Clara Cardoso
dc.contributor.authorZucolotto, Valtencir
dc.contributor.authorVieira, Lucia
dc.contributor.authorLobo, Anderson Oliveira
dc.date.accessioned2026-01-20T17:48:44Z
dc.date.available2026-01-20T17:48:44Z
dc.date.issued22024
dc.description.abstractAlzheimer’s disease is the most prevalent form of dementia and is primarily characterized by the accumulation of β-amyloid and phosphorylated tau proteins in the brain, along with the degeneration of nerve cells, which leads to impairment of various cognitive functions. A significant biomarker of Alzheimer’s disease is the decreased level of soluble β-amyloid peptide (1–42) (Aβ1-42) in cerebrospinal fluid (CSF), as pathology progresses when CSF-Aβ1-42 levels drop below 192 pg mL-1 . In this study, we developed an amperometric immunosensor based on magnetic beads as the platform for constructing the immunosensor. Monoclonal antibodies are immobilized on the MBs, enabling selective detection of Aβ1-42. The detection antibody is conjugated with the enzyme horseradish peroxidase, which, in the presence of H2O2 and hydroquinone, catalyzes the decomposition of H2O2 and the oxidation of hydroquinone to p-quinone, generating an electric current measured at a potential of −200 mV (vs. the Ag pseudo-reference electrode) using screen-printed carbon electrodes. The amperometric sandwich-type immunosensor demonstrates a linear response in the concentration range of 10 to 10,000 pg mL-1 , with a detection limit of 7.4 pg mL-1 , exhibiting excellent selectivity against the assessed interferents. These findings suggest the potential application of this immunosensor in the early diagnosis of Alzheimer’s disease, offering a sensitive and specific tool for clinical analysis. Despite its high performance, further studies are required to validate its robustness and applicability in complex clinical samples.
dc.description.physical11 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.uriFAPESP: 2015/26651-3 CNPq: 310883/2020-2; 383239/2022-2
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.affiliationUniversidade de São Carlos
dc.identifier.affiliationUniversidade Federal de Goias
dc.identifier.affiliationUniversidade de São Paulo
dc.identifier.affiliationUniversidade Federal do Piauí
dc.identifier.bibliographicCitationRAZZINO, C. A. et al. A microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection. Front. Sens., v. 5, p. 1-11, 2024. Disponível em: https://www.frontiersin.org/articles/10.3389/fsens.2024.1508810/full.
dc.identifier.doi10.3389/fsens.2024.1508810
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1098
dc.language.isoen_US
dc.publisherFrontiersin
dc.rights.holderFrontiers in Sensors
dc.subject.keywordScreen-printed electrode
dc.subject.keywordMagnetic beads
dc.subject.keywordAmperometric immunosensor
dc.subject.keywordβ-amyloid peptide (1–42)
dc.titleA microbead-enhanced electrochemical platform for β-amyloid peptide (1–42) detection
dc.typeArtigos de Periódicos

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