Incorporation of calcium aluminate and zirconia in polymethylmethacrylate (PMMA) bone cement for biomedical applications: study of in vitro osteogenesis

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dc.contributor.authorLupp, Juliana Dos Santos
dc.contributor.authorSilva, Luis Augusto de Almeida
dc.contributor.authorRamos, Nathalia de Souza
dc.contributor.authorOliveira, Ivone Regina de
dc.contributor.authorOliveira, Felipe Eduardo de
dc.contributor.authorAvelino, Sarah de Oliveira Marco
dc.contributor.authorGonçalves, Isabela dos Santos
dc.contributor.authorVaconcellos, Luana Marotta Reis de
dc.date.accessioned2026-01-14T17:53:40Z
dc.date.available2026-01-14T17:53:40Z
dc.date.issued22025
dc.description.abstractObjective: To evaluate the effects of incorporating calcium aluminate cement (CAC) and monoclinic zirconia (Z) into polymethylmethacrylate (PMMA) bone cement on in vitro osteogenesis. Material and Methods: Samples of pure PMMA, PMMA+CAC 7.5% (wt%) and PMMA+Z 7.5% (wt%) were prepared. The formulations were characterized by scanning electron microscopy, energy-dispersive spectroscopy, Fourier-transform infrared spectroscopy, and wettability analysis. For biological assessment, mesenchymal stem cells derived from Wistar rat femurs were cultured on the samples. Cellular response was analyzed by cell viability assay, protein synthesis, alkaline phosphatase (ALP) activity, tumor necrosis factor-alpha (TNF-α) expression by ELISA, and mineralized nodule formation. Results: All groups exhibited cell viability greater than 70% (p>0.05). ALP activity and protein synthesis showed no significant differences (p>0.05). TNF-α expression was significantly higher in the control group (41.25 ± 17.00 pg/mL) compared with PMMA (22.97 ± 7.08; p<0.01), PMMA+Z (25.76 ± 13.08; p<0.05), and PMMA+CAC (28.99 ± 14.71; p<0.05), suggesting inflammatory modulation. All groups presented mineralized nodules. In the wettability analysis, a numerical reduction in the contact angle was observed for PMMA+Z compared with pure PMMA, but without statistical significance (p>0.05). Conclusion: The addition of CAC and Z to PMMA resulted in more homogeneous surfaces, a trend toward increased wettability, and reduced TNF-α expression, without impairing cell viability or osteogenic potential, highlighting the promising potential of these formulations for bone regeneration.
dc.description.physical16 p.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.uriFAPESP: 2021/09367-0
dc.format.mimetypePDF
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationLUPP, J. S. et al. Incorporation of calcium aluminate and zirconia in polymethylmethacrylate (PMMA) bone cement for biomedical applications: study of in vitro osteogenesis. Brazilian Dental Science, v. 28, n. 4, p. 1-16, 2025. Disponível em: https://ojs.ict.unesp.br/index.php/cob/article/view/4780.
dc.identifier.doi10.4322/bds.2025.e4780
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/1088
dc.language.isoen_US
dc.publisherUniversidade Estadual Paulista
dc.rights.holderBrazilian Dental Science
dc.subject.keywordBone cement
dc.subject.keywordCalcium aluminate
dc.subject.keywordOsteogenesis
dc.subject.keywordPolymethyl methacrylate
dc.subject.keywordZirconia
dc.titleIncorporation of calcium aluminate and zirconia in polymethylmethacrylate (PMMA) bone cement for biomedical applications: study of in vitro osteogenesis
dc.title.alternativeIncorporação do aluminato de cálcio e da zircônia no cimento ósseo de polimetilmetacrilato (PMMA) para aplicações biomédicas: estudo da osteogênese in vitro
dc.typeArtigos de Periódicos

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