Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma

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dc.contributor.authorVieira, Paula Fonseca Antunes
dc.contributor.authorJesus, Viviane Paula dos Santos
dc.contributor.authorCândido, Marcela Aparecida
dc.contributor.authorPacheco-Soares, Cristina
dc.contributor.authorCastilho, Maiara Lima
dc.contributor.authorRaniero, Leandro José
dc.date.accessioned2025-04-01T18:17:15Z
dc.date.available2025-04-01T18:04:28Z
dc.date.available2025-04-01T18:17:15Z
dc.date.issued22022-07-01
dc.description.abstractBackground: Epidermal Growth Factor Receptor (EGFR receptor) is encoded by the EGFR gene. EGFR receptor signaling pathways are activated by EGF protein, regulating cell actions. Overexpression of EGFR receptor may be linked to malignancies with a poor prognosis. As a result, EGFR receptor is being studied for a variety of tumor diagnostics, spurring the development of innovative approaches to increase quality and efficiency. Nanomaterials can recognize cancer cells by specifically targeting of molecular pathways, underscoring the importance of nanomedicine. In this study, we synthesized EGFR-specific nanomarkers by functionalizing EGF protein and Chlorin e6 in gold nanoparticles. These nanoparticles use active targeting to deliver EGF protein to EGFR receptor, and Chlorin e6 serves as a fluorescent marker molecule Methods: Nanomarkers were examined in vitro in MDA-MB-468 and M059J cell lines. Confocal microscopy and flow cytometry were used to examine the distribution, uptake, internalization, and fluorescence intensity of nanomarkers in vitro Results: The results show that both lines examined accumulate nanomarkers. However, MDA-MB-468 had the highest intensity due to its EGFR receptor overexpression properties Conclusion: The findings point to ideal properties for detecting EGFR receptor overexpressed cells.
dc.description.physical7 p.
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (Finep)
dc.description.uriPIBIC/CNPq (44963/2018–3 and 126218/2019–6), CAPES (88887.649530/2021–00). FINEP/MCTIC [grant number 01.13.0275.00]. FAPESP [grant number 2017/07519–2]; CNPq [grant number 302944/2018–4].
dc.format.mimetypepdf
dc.identifier.affiliationUniversidade do Vale do Paraíba
dc.identifier.bibliographicCitationVIEIRA, P. et al. Specific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma. Photodiagnosis and Photodynamic Therapy, [S. l.], v. 39, p. 102997, set. 2022. Disponível em: https://linkinghub.elsevier.com/retrieve/pii/S1572100022002836.
dc.identifier.doi10.1016/j.pdpdt.2022.102997
dc.identifier.urihttps://repositorio.univap.br/handle/123456789/795.2
dc.language.isoen_US
dc.publisherElsevier
dc.rights.holderElsevier B.V.
dc.subject.keywordNanomedicine
dc.subject.keywordEGF
dc.subject.keywordCe6
dc.subject.keywordFluorescence
dc.titleSpecific nanomarkers fluorescence: in vitro analysis for EGFR overexpressed cells in triple-negative breast cancer and malignant glioblastoma
dc.typeArtigos de Periódicos

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